| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,100 | G→A | 8.9% | D718D (GAC→GAT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,100 | 0 | G | A | 8.9% | 212.6 / 7.4 | 67 | D718D (GAC→GAT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (26/35); new base A (3/3); total (29/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT > minE/608030‑608170 | aGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcgcg < 1:2970336/71‑1 (MQ=255) gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTc > 1:3850627/1‑69 (MQ=255) aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag > 1:255551/1‑69 (MQ=255) cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt < 1:2941602/71‑1 (MQ=255) cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt > 1:3755243/1‑71 (MQ=255) cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt > 1:833715/1‑71 (MQ=255) gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt > 1:789006/1‑69 (MQ=255) gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt > 1:1781679/1‑69 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg < 1:3759950/70‑1 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg < 1:2782407/70‑1 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg < 1:4059636/70‑1 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg < 1:1554197/70‑1 (MQ=255) gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt > 1:2263107/1‑71 (MQ=255) ccAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt > 1:678018/1‑71 (MQ=255) tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag > 1:2113819/1‑51 (MQ=255) tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc > 1:2117928/1‑62 (MQ=255) tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatca > 1:2134546/1‑71 (MQ=255) cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg < 1:3307487/61‑1 (MQ=255) cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:954018/70‑1 (MQ=255) cGGATGGTCAGCT‑‑GGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt > 1:3782096/1‑59 (MQ=38) ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa < 1:2867888/71‑1 (MQ=255) ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa < 1:2862875/71‑1 (MQ=255) gATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttga < 1:2025127/71‑3 (MQ=255) gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa < 1:3732417/71‑1 (MQ=255) gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc > 1:2968005/1‑60 (MQ=255) gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:1895377/71‑1 (MQ=255) tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc < 1:2209672/71‑1 (MQ=255) cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt > 1:341219/1‑69 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg > 1:873134/1‑51 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa < 1:428259/70‑1 (MQ=255) taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt > 1:1509493/3‑42 (MQ=12) taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt > 1:2042885/3‑70 (MQ=255) cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat < 1:3068170/51‑1 (MQ=255) cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg < 1:1592085/61‑1 (MQ=255) cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt < 1:2993827/71‑1 (MQ=255) aggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt > 1:1240154/2‑69 (MQ=255) ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTg < 1:808871/71‑1 (MQ=12) gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt < 1:3237633/46‑1 (MQ=255) gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa > 1:87146/1‑71 (MQ=255) gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt < 1:2344523/46‑1 (MQ=12) atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:2751272/60‑1 (MQ=255) atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac > 1:2458871/1‑71 (MQ=255) tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat < 1:2823193/45‑1 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa > 1:1845417/1‑57 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt > 1:3168975/1‑63 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc > 1:747842/1‑70 (MQ=255) ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc < 1:2698739/70‑1 (MQ=255) ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc < 1:1257222/70‑1 (MQ=255) cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg < 1:2694289/57‑1 (MQ=255) cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc < 1:1602284/63‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:662534/71‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:757415/71‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:2205812/71‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:3973057/48‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:178005/48‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg > 1:1728911/1‑70 (MQ=255) ttttCCATCGCGTCGAGCATCATTTCACGGTTCATCACGTTAACGtt < 1:3039242/47‑1 (MQ=255) ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc < 1:2731694/48‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:2926582/37‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:2931425/37‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:1306678/37‑1 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:319676/1‑71 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:1585647/1‑71 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat > 1:3293199/1‑70 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:1791464/67‑1 (MQ=255) tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc > 1:994847/1‑71 (MQ=255) ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt > 1:1545759/1‑71 (MQ=255) tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg < 1:637657/46‑1 (MQ=255) gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga > 1:1281253/1‑58 (MQ=255) gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg > 1:789870/1‑71 (MQ=255) gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt > 1:3071801/1‑71 (MQ=255) | AGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGT > minE/608030‑608170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |