Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,103	G→C	8.6%	L717L (CTC→CTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,103	0	G	C	8.6%	209.3 / 8.0	70	L717L (CTC→CTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (28/36);  new base C (3/3);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGG  >  minE/608037‑608172                                                                   |                                                                      aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                                     >  1:255551/1‑69 (MQ=255)      cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                              >  1:833715/1‑71 (MQ=255)      cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                              <  1:2941602/71‑1 (MQ=255)      cTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                              >  1:3755243/1‑71 (MQ=255)        gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                              >  1:789006/1‑69 (MQ=255)        gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAtt                                                              >  1:1781679/1‑69 (MQ=255)             gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                        <  1:2782407/70‑1 (MQ=255)             gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                        <  1:4059636/70‑1 (MQ=255)             gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                        <  1:1554197/70‑1 (MQ=255)             gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                        <  1:3759950/70‑1 (MQ=255)             gCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                                       >  1:2263107/1‑71 (MQ=255)              ccAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                                      >  1:678018/1‑71 (MQ=255)                   tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                                                                     >  1:2113819/1‑51 (MQ=255)                   tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCAc                                                          >  1:2117928/1‑62 (MQ=255)                   tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatca                                                 >  1:2134546/1‑71 (MQ=255)                     cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACg                                                         <  1:3307487/61‑1 (MQ=255)                     cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                <  1:954018/70‑1 (MQ=255)                       cGGATGGTCAGCT‑‑GGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGt                                                       >  1:3782096/1‑59 (MQ=38)                        ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                                            <  1:2862875/71‑1 (MQ=255)                        ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa                                            <  1:2867888/71‑1 (MQ=255)                         gATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttga                                           <  1:2025127/71‑3 (MQ=255)                         gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa                                           <  1:3732417/71‑1 (MQ=255)                             gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc                                                  >  1:2968005/1‑60 (MQ=255)                             gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       <  1:1895377/71‑1 (MQ=255)                              tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                      <  1:2209672/71‑1 (MQ=255)                               cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       >  1:341219/1‑69 (MQ=255)                                aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACgg                                                        >  1:873134/1‑51 (MQ=255)                                aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                                     <  1:428259/70‑1 (MQ=255)                                    taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt                                                             >  1:1509493/3‑42 (MQ=12)                                    taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt                                >  1:2042885/3‑70 (MQ=255)                                     cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                   <  1:3068170/51‑1 (MQ=255)                                     cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg                                         <  1:1592085/61‑1 (MQ=255)                                     cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                               <  1:2993827/71‑1 (MQ=255)                                     aggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt                               >  1:1240154/2‑69 (MQ=255)                                      ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTg                              <  1:808871/71‑1 (MQ=12)                                       gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGtt                                                      <  1:3237633/46‑1 (MQ=255)                                       gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa                             >  1:87146/1‑71 (MQ=255)                                       gATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAtt                                                      <  1:2344523/46‑1 (MQ=12)                                        atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       <  1:2751272/60‑1 (MQ=255)                                        atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac                            >  1:2458871/1‑71 (MQ=255)                                           tttttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcat                                                   <  1:2823193/45‑1 (MQ=255)                                             tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa                                     >  1:1845417/1‑57 (MQ=255)                                             tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt                               >  1:3168975/1‑63 (MQ=255)                                             tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc                        >  1:747842/1‑70 (MQ=255)                                                ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc                     <  1:2698739/70‑1 (MQ=255)                                                ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc                     <  1:1257222/70‑1 (MQ=255)                                                 cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                 <  1:2694289/57‑1 (MQ=255)                                                 cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc                           <  1:1602284/63‑1 (MQ=255)                                                   ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                 <  1:2205812/71‑1 (MQ=255)                                                   ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                 <  1:662534/71‑1 (MQ=255)                                                   ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                 <  1:757415/71‑1 (MQ=255)                                                    gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       <  1:3973057/48‑1 (MQ=255)                                                    gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt                                       <  1:178005/48‑1 (MQ=255)                                                    gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                 >  1:1728911/1‑70 (MQ=255)                                                     ttttCCATCGCGTCGAGCATCATTTCACGGTTCATCACGTTAACGtt                                       <  1:3039242/47‑1 (MQ=255)                                                     ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc                                      <  1:2731694/48‑1 (MQ=255)                                                      tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                <  1:2926582/37‑1 (MQ=255)                                                      tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                <  1:2931425/37‑1 (MQ=255)                                                      tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc                                                <  1:1306678/37‑1 (MQ=255)                                                       ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg             >  1:1585647/1‑71 (MQ=255)                                                       ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg             >  1:319676/1‑71 (MQ=255)                                                       ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat              >  1:3293199/1‑70 (MQ=255)                                                       ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg                 <  1:1791464/67‑1 (MQ=255)                                                        tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc            >  1:994847/1‑71 (MQ=255)                                                         ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt           >  1:1545759/1‑71 (MQ=255)                                                            tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg                                 <  1:637657/46‑1 (MQ=255)                                                              gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga                   >  1:1281253/1‑58 (MQ=255)                                                              gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg      >  1:789870/1‑71 (MQ=255)                                                                gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt    >  1:3071801/1‑71 (MQ=255)                                                                 tCGATCAGCATTTCACGGTTCATCACGTTAACGTTCa                                     <  1:1402645/37‑1 (MQ=255)                                                                   gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga               >  1:2311773/1‑57 (MQ=255)                                                                   gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg      >  1:1126238/1‑66 (MQ=255)                                                                   gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  >  1:3085286/1‑70 (MQ=255)                                                                   gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg  >  1:1333066/1‑70 (MQ=255)                                                                   |                                                                      ACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGG  >  minE/608037‑608172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑