| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,124 | C→T | 7.0% | V710V (GTG→GTA) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,124 | 0 | C | T | 7.0% | 233.7 / 4.8 | 71 | V710V (GTG→GTA) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (34/32); new base T (3/2); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAG > minE/608055‑608194 | tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatca > 1:2134546/1‑71 (MQ=255) cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:954018/70‑1 (MQ=255) ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa < 1:2867888/71‑1 (MQ=255) ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTa < 1:2862875/71‑1 (MQ=255) gATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGttga < 1:2025127/71‑3 (MQ=255) gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa < 1:3732417/71‑1 (MQ=255) gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc > 1:2968005/1‑60 (MQ=255) gTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:1895377/71‑1 (MQ=255) tCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc < 1:2209672/71‑1 (MQ=255) cAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt > 1:341219/1‑69 (MQ=255) aGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa < 1:428259/70‑1 (MQ=255) taggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgt > 1:2042885/3‑70 (MQ=255) cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACg < 1:1592085/61‑1 (MQ=255) cGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt < 1:2993827/71‑1 (MQ=255) aggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTgtt > 1:1240154/2‑69 (MQ=255) ggATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGAGGTGTTg < 1:808871/71‑1 (MQ=12) gATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGa > 1:87146/1‑71 (MQ=255) atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:2751272/60‑1 (MQ=255) atatTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGac > 1:2458871/1‑71 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCa > 1:1845417/1‑57 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCt > 1:3168975/1‑63 (MQ=255) tttCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGaccacc > 1:747842/1‑70 (MQ=255) ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc < 1:2698739/70‑1 (MQ=255) ccGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc < 1:1257222/70‑1 (MQ=255) cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg < 1:2694289/57‑1 (MQ=255) cGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGacc < 1:1602284/63‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:2205812/71‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:662534/71‑1 (MQ=255) ggTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:757415/71‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:178005/48‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGtt < 1:3973057/48‑1 (MQ=255) gTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg > 1:1728911/1‑70 (MQ=255) ttttCCATCGCGTCGAGCATCATTTCACGGTTCATCACGTTAACGtt < 1:3039242/47‑1 (MQ=255) ttttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTc < 1:2731694/48‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:1306678/37‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:2931425/37‑1 (MQ=255) tttCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc < 1:2926582/37‑1 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:319676/1‑71 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:1585647/1‑71 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggat > 1:3293199/1‑70 (MQ=255) ttCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatg < 1:1791464/67‑1 (MQ=255) tCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGc > 1:994847/1‑71 (MQ=255) ccATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCt > 1:1545759/1‑71 (MQ=255) tCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTg < 1:637657/46‑1 (MQ=255) gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCga > 1:1281253/1‑58 (MQ=255) gcgTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg > 1:789870/1‑71 (MQ=255) gTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt > 1:3071801/1‑71 (MQ=255) tCGATCAGCATTTCACGGTTCATCACGTTAACGTTCa < 1:1402645/37‑1 (MQ=255) gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga > 1:2311773/1‑57 (MQ=255) gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtg > 1:1126238/1‑66 (MQ=255) gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg > 1:3085286/1‑70 (MQ=255) gAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGtggtgg > 1:1333066/1‑70 (MQ=255) agcaTTTCCCGATTCATTACGTTGACGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGaaa > 1:591115/1‑68 (MQ=12) gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga > 1:856523/1‑52 (MQ=255) gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga > 1:65986/1‑52 (MQ=255) gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga > 1:58018/1‑52 (MQ=255) gcaTTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTaa < 1:91753/71‑1 (MQ=255) tttCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTc < 1:2349467/43‑1 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGacca > 1:1106761/1‑36 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGacca > 1:511709/1‑36 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:3355235/1‑49 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatggatg > 1:3546612/1‑49 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCgtggt > 1:1086844/1‑58 (MQ=255) tCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatc > 1:1453375/1‑71 (MQ=255) cACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatc > 1:924633/1‑70 (MQ=255) cGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat < 1:2671877/71‑1 (MQ=255) ggctcatcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat < 1:3003433/67‑1 (MQ=255) ggTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCgatgga > 1:581999/1‑43 (MQ=255) ggTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat > 1:2027532/1‑70 (MQ=255) ggTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat > 1:1273188/1‑70 (MQ=255) ggTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat > 1:1601783/1‑70 (MQ=255) ggTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatccat > 1:354624/1‑70 (MQ=255) gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa < 1:1962647/71‑1 (MQ=255) gTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCa < 1:585938/71‑1 (MQ=255) tcatcaCGTTAACGTTCAGGTGCTGACCACCTTCgatg > 1:2193590/1‑38 (MQ=255) tcatcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAccatc > 1:1791889/1‑64 (MQ=255) tcatcaCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCCTCGTGGTGGAAGTAAc > 1:3146625/1‑60 (MQ=255) catcaCGTTAAAGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGa < 1:2986580/70‑1 (MQ=255) caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGaa > 1:1169785/1‑51 (MQ=255) caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAAcc > 1:400568/1‑57 (MQ=255) caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag > 1:4059787/1‑71 (MQ=255) caCGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGAccag > 1:2253541/1‑71 (MQ=255) | TACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAG > minE/608055‑608194 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |