Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,895 T→C 9.4% E453E (GAA→GAG pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,8950TC 9.4% 265.5 / 16.6 96E453E (GAA→GAGpflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (35/52);  new base C (4/5);  total (39/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 107 total aligned reads displayed.

TCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTG  >  minE/608825‑608963
                                                                      |                                                                    
tCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGtttt                                                                      <  1:1003753/71‑1 (MQ=255)
 cACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGttttt                                                                     >  1:1600743/1‑71 (MQ=255)
  aCTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGttttt                                                                     >  1:704688/1‑70 (MQ=255)
  aCTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTc                                                                    >  1:3898571/1‑71 (MQ=255)
  aCTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTc                                                                    >  1:1941100/1‑71 (MQ=255)
   cTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCg                                                                   <  1:1599451/71‑1 (MQ=255)
   cTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCg                                                                   <  1:1325117/71‑1 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGt                                                                  <  1:2748844/69‑1 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 >  1:2463515/1‑70 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 <  1:3666135/70‑1 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 <  1:600650/70‑1 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 >  1:1806489/1‑70 (MQ=255)
      catcatAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 <  1:654914/70‑1 (MQ=255)
          atAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGttttt                                                                     >  1:2886941/1‑62 (MQ=255)
          atAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAcgc                                                            >  1:1091706/1‑71 (MQ=255)
               tCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:1676283/70‑1 (MQ=255)
                cAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTc                                                                    >  1:3849779/1‑57 (MQ=255)
                cAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGt                                                      >  1:3908735/1‑71 (MQ=255)
                cAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGt                                                      >  1:3295514/1‑71 (MQ=255)
                 aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:2494603/68‑1 (MQ=255)
                 aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                     <  1:1245158/71‑1 (MQ=255)
                 aGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                     <  1:368580/71‑1 (MQ=255)
                  ggACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                    <  1:3280851/71‑1 (MQ=255)
                  ggACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                    <  1:1808459/71‑1 (MQ=255)
                  ggACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                    <  1:355522/71‑1 (MQ=255)
                   gACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        >  1:2014325/1‑66 (MQ=255)
                    aCATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGAt                                                  >  1:3088825/1‑71 (MQ=255)
                     cATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGAtt                                                 >  1:3686774/1‑71 (MQ=255)
                       tCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCg                                                                   <  1:2366769/51‑1 (MQ=255)
                       tCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                    <  1:1961296/66‑1 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 <  1:3384560/51‑1 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 <  1:498474/51‑1 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTc                                                                 >  1:2560991/1‑51 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAc                                                              >  1:2949624/1‑54 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCg                                              <  1:2592926/70‑1 (MQ=255)
                         gCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCg                                              >  1:3643924/1‑70 (MQ=255)
                          ccTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTa                                            <  1:3931434/71‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCATCCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:2740702/58‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:3753135/58‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:2102384/58‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACgccgcc                                                        <  1:3593097/58‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTAc                                           <  1:255345/71‑1 (MQ=255)
                           cTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTAc                                           <  1:1548144/71‑1 (MQ=255)
                            tttGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCaa                                                               <  1:1231372/50‑1 (MQ=255)
                              tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         >  1:3777530/1‑70 (MQ=255)
                              tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         >  1:344849/1‑70 (MQ=255)
                              tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         >  1:447005/1‑70 (MQ=255)
                              tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         >  1:3899270/1‑70 (MQ=255)
                              tGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         >  1:271456/1‑70 (MQ=255)
                                aTCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCAt                                      <  1:3374726/71‑1 (MQ=255)
                                 tCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                     >  1:3559816/1‑71 (MQ=255)
                                  cGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATgg                                    >  1:3071503/1‑71 (MQ=255)
                                   ggTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGtt                                                     >  1:1746406/1‑53 (MQ=255)
                                   ggTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGt                                   <  1:1785360/71‑1 (MQ=255)
                                    gTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAAcgccgc                                                         <  1:4080758/48‑1 (MQ=255)
                                     ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCg                                                                   <  1:1304130/37‑1 (MQ=255)
                                     ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCg                                                                   <  1:3979470/37‑1 (MQ=255)
                                     ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATgg                                    <  1:1368281/68‑1 (MQ=255)
                                      tCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGccgccg                                                       >  1:3150346/1‑48 (MQ=255)
                                       cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                               >  1:962584/1‑71 (MQ=255)
                                       cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                               >  1:508069/1‑71 (MQ=255)
                                        aGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCg                                              <  1:2526462/55‑1 (MQ=255)
                                           cTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                     >  1:1254041/1‑61 (MQ=255)
                                            ttCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                          >  1:3006853/1‑71 (MQ=255)
                                            ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAAc                                                              <  1:2367990/35‑1 (MQ=255)
                                            ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTg                                                    <  1:2825671/45‑1 (MQ=255)
                                             tCGGCCCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                         >  1:1510627/1‑71 (MQ=255)
                                             tCGGACCAACCTGCATTTTCAGTTTTTCGTCAACTCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                         >  1:2552676/1‑71 (MQ=255)
                                                 aCCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                     <  1:1038274/71‑1 (MQ=255)
                                                 aCCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                     <  1:2792016/71‑1 (MQ=255)
                                                 aCCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                     <  1:1971130/71‑1 (MQ=255)
                                                 aCCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTg                     <  1:2491041/71‑1 (MQ=255)
                                                  ccgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAgg                         >  1:3826218/4‑66 (MQ=255)
                                                   cgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtta                      >  1:1234575/3‑67 (MQ=255)
                                                    aaCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                  <  1:1016534/71‑1 (MQ=255)
                                                    aaCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                  <  1:3040962/71‑1 (MQ=255)
                                                    aaCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                  <  1:2910320/71‑1 (MQ=255)
                                                    aaCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAc                  <  1:2681248/71‑1 (MQ=255)
                                                     aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                 >  1:1329070/1‑71 (MQ=255)
                                                      ccTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgc                >  1:2043102/1‑71 (MQ=255)
                                                        tGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                         <  1:1021816/44‑1 (MQ=255)
                                                        tGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg               >  1:24167/1‑70 (MQ=255)
                                                          cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACgcgc              <  1:1895898/69‑1 (MQ=255)
                                                              ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg          >  1:3654931/1‑69 (MQ=255)
                                                              ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg          >  1:2381497/1‑69 (MQ=255)
                                                                cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag       <  1:27440/70‑1 (MQ=255)
                                                                cAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag       <  1:102482/70‑1 (MQ=255)
                                                                cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAccaa         <  1:1916967/68‑3 (MQ=255)
                                                                cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca          <  1:3618961/67‑2 (MQ=255)
                                                                cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag       <  1:1703505/70‑1 (MQ=255)
                                                                cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag       <  1:1401060/70‑1 (MQ=255)
                                                                 aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa     >  1:595649/1‑71 (MQ=255)
                                                                 aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaaga      <  1:2728383/70‑1 (MQ=255)
                                                                 aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaaga      <  1:3160660/70‑1 (MQ=255)
                                                                  gTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc    <  1:2532648/71‑1 (MQ=255)
                                                                   tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGAACGCGCACCGAAGAACt   >  1:2008479/1‑71 (MQ=255)
                                                                    ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg  >  1:3852655/1‑71 (MQ=255)
                                                                     tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                 >  1:1668574/1‑55 (MQ=255)
                                                                     tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg  >  1:3339379/1‑70 (MQ=255)
                                                                      ttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag       >  1:229808/1‑64 (MQ=255)
                                                                      |                                                                    
TCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTG  >  minE/608825‑608963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: