| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 15,386 | C→T | 19.0% | intergenic (+88/+59) | dnaJ → / ← mokC | chaperone Hsp40, co‑chaperone with DnaK/regulatory protein for HokC, overlaps CDS of hokC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 15,386 | 0 | C | T | 19.0% | 114.8 / 21.3 | 53 | intergenic (+88/+59) | dnaJ/mokC | chaperone Hsp40, co‑chaperone with DnaK/regulatory protein for HokC, overlaps CDS of hokC |
| Reads supporting (aligned to +/- strand): ref base C (23/18); new base T (5/5); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCTCTTCTC > minE/15318‑15456 | gTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTc < 1:1515669/71‑1 (MQ=255) gCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCAt < 1:386441/71‑1 (MQ=255) gctgtctgcgtcaatcgcagcactggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAg < 1:1957464‑M1/11‑1 (MQ=255) cTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgcgc > 1:3060669/1‑71 (MQ=255) ggTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAgcgat < 1:1826134/69‑3 (MQ=255) ggTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTa < 1:961201/70‑1 (MQ=255) tAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACt > 1:3309587/1‑71 (MQ=255) tAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACt > 1:3860129/1‑71 (MQ=255) aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACtt < 1:787139/70‑1 (MQ=255) aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa < 1:2997661/71‑1 (MQ=255) aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa < 1:131474/71‑1 (MQ=255) aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTa < 1:1724117/71‑1 (MQ=255) aaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAg > 1:4110892/1‑71 (MQ=255) tAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAggg > 1:1855796/1‑71 (MQ=255) tAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAggg > 1:3488915/1‑71 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACt > 1:1553591/1‑47 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGtt > 1:2225032/1‑71 (MQ=255) gggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGt < 1:2633244/70‑1 (MQ=255) ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt < 1:2807949/71‑1 (MQ=255) ttGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt < 1:214050/71‑1 (MQ=255) tggtgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt < 1:1449256‑M1/34‑1 (MQ=255) tGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCAc > 1:2485265/1‑38 (MQ=255) gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAAc > 1:4055419/1‑53 (MQ=255) gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg > 1:1591709/1‑66 (MQ=255) tgctgtaagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTAc < 1:1164800‑M1/37‑1 (MQ=255) aaGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTcc > 1:660592/1‑39 (MQ=255) aGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACg > 1:505865/1‑71 (MQ=255) tatGCGAGCACCTGTAAAGTGGCGGGGATCAATCCCATAAGCGCTAACTTAAGGGTtgt > 1:2223481/1‑59 (MQ=255) taagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCt > 1:1726692‑M1/30‑70 (MQ=255) taagaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCt > 1:903766‑M1/30‑70 (MQ=255) tGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt < 1:3290071/62‑1 (MQ=255) agaccatacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGcc < 1:961605‑M1/40‑1 (MQ=255) gAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGatat < 1:1743659/71‑1 (MQ=255) gCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTAt < 1:49565/57‑1 (MQ=255) catacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTgg > 1:2678584‑M1/24‑54 (MQ=255) cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGtt > 1:1168423/1‑49 (MQ=255) tacataaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAtt < 1:2336033‑M1/50‑1 (MQ=255) ccTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg < 1:123433/48‑1 (MQ=255) cTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTa < 1:3123788/71‑1 (MQ=255) tGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg < 1:181238/49‑1 (MQ=255) tGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg < 1:50531/49‑1 (MQ=255) taaaaaagccggggataATTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGa > 1:3364167‑M1/18‑60 (MQ=255) aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgt > 1:2453078/1‑71 (MQ=255) aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcg > 1:2677895/1‑70 (MQ=255) aaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGc > 1:4111992/1‑53 (MQ=255) gTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAAcgtgcc > 1:943650/1‑71 (MQ=255) gCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg > 1:2475878/1‑37 (MQ=40) gCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtg > 1:1528640/1‑37 (MQ=40) cggggagtAATCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTaacgtgccgat > 1:1108840‑M1/9‑68 (MQ=255) gataATTCCCATAAGCGCTAACTTAAGGGTTGTgg > 1:735675‑M1/5‑35 (MQ=255) cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGctc > 1:14398/1‑66 (MQ=255) cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGctc > 1:483374/1‑66 (MQ=255) ACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCtcttc > 1:2821423‑M1/1‑70 (MQ=255) ACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCtcttc > 1:3025373‑M1/1‑70 (MQ=255) cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCTCTtctc < 1:908813/71‑1 (MQ=255) | GTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGTGCTCTCTTCTC > minE/15318‑15456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |