| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 660,873 | G→A | 16.6% | V76V (GTG→GTA) | ycbF → | predicted periplasmic pilini chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 660,873 | 0 | G | A | 16.6% | 56.5 / 4.3 | 24 | V76V (GTG→GTA) | ycbF | predicted periplasmic pilini chaperone |
| Reads supporting (aligned to +/- strand): ref base G (5/15); new base A (2/2); total (7/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTG > minE/660810‑660942 | cTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAc > 1:2082974/1‑71 (MQ=255) ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAg > 1:2473976/1‑60 (MQ=255) aaaTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTACCAGCACCATTACTGGCCGCTCCg > 1:2053594/1‑71 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc > 1:832407/1‑70 (MQ=255) aaCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTACCAGCAcc < 1:2361143/50‑1 (MQ=255) aaCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTACCAGCACCATTACTGGCCGCTCCGCCACt > 1:414500/1‑71 (MQ=255) cGCCCAACGGGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:1488591/39‑1 (MQ=255) cGCCCAACGCGTGGGGCCGGGGTGGAAGCGGCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc < 1:2065874/65‑1 (MQ=255) ttGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGcc < 1:4093854/46‑1 (MQ=255) ttGGGCC‑GGGTGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGcc < 1:992161/71‑1 (MQ=255) tGGGCCGGGGGGGAAGCGTCGACAGTACCAGCACCATTACt < 1:2462314/41‑1 (MQ=255) gCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc < 1:2823946/49‑1 (MQ=255) cGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGc < 1:3662519/42‑1 (MQ=255) cGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTc < 1:3681943/44‑1 (MQ=255) cGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtct < 1:3814014/57‑1 (MQ=255) gggggAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa < 1:3650069/71‑1 (MQ=255) ggggAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa < 1:3701992/70‑1 (MQ=255) gggAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa < 1:3374172/69‑1 (MQ=255) gAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt < 1:1463219/45‑1 (MQ=255) gCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTAc < 1:3493556/62‑1 (MQ=255) cGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGc < 1:3497934/68‑1 (MQ=255) cGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTa > 1:995139/1‑39 (MQ=255) cAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCa > 1:1704236/1‑63 (MQ=255) aGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACg < 1:930404/55‑1 (MQ=255) tGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTg < 1:2121298/71‑1 (MQ=255) | CTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTG > minE/660810‑660942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |