| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 705,295 | A→G | 18.4% | G32G (GGT→GGC) | ymcB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 705,295 | 0 | A | G | 18.4% | 220.0 / 43.6 | 98 | G32G (GGT→GGC) | ymcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (35/45); new base G (7/11); total (42/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTG > minE/705225‑705361 | cgcgCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCa > 1:2746375/1‑71 (MQ=255) ttGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCa < 1:3472183/71‑1 (MQ=255) tGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCa < 1:1203374/70‑1 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGAt < 1:2274008/68‑1 (MQ=255) ttGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa < 1:1282454/71‑1 (MQ=255) tgtgTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa > 1:2645319/1‑70 (MQ=255) gtgtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAcc > 1:1241686/1‑58 (MQ=255) gtgtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa > 1:3528927/1‑69 (MQ=255) tgtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa < 1:783976/70‑1 (MQ=255) gtCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGt > 1:872385/1‑71 (MQ=255) tCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGt > 1:1941369/1‑70 (MQ=255) tCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGt < 1:3597261/70‑1 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCa > 1:1382098/1‑70 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCAc > 1:3686727/1‑71 (MQ=255) aCCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGt > 1:1456012/1‑68 (MQ=255) aCTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGAt < 1:4073570/57‑1 (MQ=255) tGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCa > 1:1482620/1‑45 (MQ=255) tGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCa > 1:1531475/1‑45 (MQ=255) tGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCa < 1:2504169/52‑1 (MQ=255) ggACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCa > 1:3513257/1‑44 (MQ=255) ggACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAg > 1:1749820/1‑60 (MQ=255) ggACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAg > 1:1895063/1‑60 (MQ=255) ggACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCt > 1:169064/1‑71 (MQ=255) gACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCtt > 1:2090653/1‑71 (MQ=255) acaacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAc < 1:672483/43‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTc < 1:1615723/66‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgc < 1:3359878/71‑1 (MQ=255) caacaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgc < 1:4028444/71‑1 (MQ=255) acaTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATa < 1:3074285/50‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:755283/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:298600/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTCCGCa < 1:2123715/71‑1 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc > 1:2574141/1‑70 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc > 1:3126149/1‑70 (MQ=255) caTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc > 1:1125215/1‑70 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGa < 1:898051/46‑1 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTgcgc > 1:1017828/1‑69 (MQ=255) aTTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa > 1:1012953/1‑70 (MQ=255) ttttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaaa < 1:1108265/49‑1 (MQ=255) tttCCACGGGGCCAACGGAAAGTGTCAGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAg > 1:684618/1‑71 (MQ=255) ttCCACGGGGCCAACGGAAAGTGTCTGTTGTTCAc > 1:3346676/1‑35 (MQ=255) tCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:346573/1‑48 (MQ=255) tCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATa > 1:3220996/1‑48 (MQ=255) tCCACGGGGCCAACGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:728139/70‑1 (MQ=255) tCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:2232568/70‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATaa < 1:690886/44‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:1736904/69‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:528228/69‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc < 1:4033203/70‑1 (MQ=255) ccACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc < 1:245805/70‑1 (MQ=255) ggCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgt < 1:3229876/71‑1 (MQ=255) gCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAAt > 1:1264203/1‑39 (MQ=255) aCGTAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCtt < 1:444375/49‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCtt < 1:1255738/49‑1 (MQ=255) aCGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt < 1:152846/71‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATg < 1:546343/61‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATg < 1:443937/61‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:2438729/53‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:2648695/59‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa < 1:81604/59‑1 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt > 1:3084002/1‑70 (MQ=255) cGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCAt > 1:4024565/1‑70 (MQ=255) ggAAAGTGTCTGTTGTTGACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTa > 1:3603196/1‑71 (MQ=255) ggAAAGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCa > 1:471474/1‑58 (MQ=255) ggAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTa > 1:3684149/1‑71 (MQ=255) gAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCtt < 1:527600/46‑1 (MQ=255) aaaGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc > 1:1347806/1‑55 (MQ=255) aaaGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGc > 1:1325549/1‑55 (MQ=255) aaaGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATggg < 1:2125473/60‑1 (MQ=255) aaGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACg > 1:3246406/1‑70 (MQ=255) aGTGTCTGTTGTTCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:3751287/48‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCATATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:2296055/55‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:1882768/55‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:2713888/55‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:2420925/55‑1 (MQ=255) aGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:133461/71‑1 (MQ=255) tgtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAgg < 1:1947606/50‑1 (MQ=255) gtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa > 1:3794354/1‑68 (MQ=255) gtCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa > 1:1330468/1‑68 (MQ=255) tCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt < 1:2009659/51‑1 (MQ=255) tCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTaaa < 1:432375/69‑1 (MQ=255) tCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAg < 1:3048419/70‑1 (MQ=255) tCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAg < 1:1117459/70‑1 (MQ=255) tCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAg < 1:2579639/70‑1 (MQ=255) tgttgtTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAg > 1:67971/1‑45 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:4055154/63‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTa < 1:1514486/63‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTa < 1:2638033/68‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTa < 1:68710/68‑1 (MQ=255) ttgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGc > 1:1769310/1‑71 (MQ=255) tgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgggg > 1:3394658/1‑50 (MQ=255) tgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgggg > 1:2051746/1‑50 (MQ=255) tgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATgggg > 1:160171/1‑50 (MQ=255) tgttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCt > 1:2367881/1‑71 (MQ=255) gttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTg > 1:3989922/1‑71 (MQ=255) gttCGCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTg > 1:3347121/1‑71 (MQ=255) ttCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCa < 1:3083997/37‑1 (MQ=255) cACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCAt > 1:664525/1‑42 (MQ=255) aCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGgtgt < 1:3997921/48‑1 (MQ=255) aCCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTg < 1:4029494/67‑1 (MQ=255) | CGCGCAGTTGCGGTTGTGTCACCAACTGGACAACATTTTCCACGGGGCCAACGGAAAGTGTCTGTTGTTCACCAGGCAGATAAATAGTCACCGTTCCTTGCGCAAAGGCATGGGGTGTCATTACGTAAAGTACGCTG > minE/705225‑705361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |