| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 706,680 | C→T | 11.5% | R244K (AGG→AAG) | lpxL ← | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 706,680 | 0 | C | T | 11.5% | 175.0 / 11.4 | 61 | R244K (AGG→AAG) | lpxL | lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (33/21); new base T (4/3); total (37/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01 | |||||||||||
TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > minE/706611‑706750 | tCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGcc < 1:235109/70‑1 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCg < 1:3346128/69‑1 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCg < 1:3149342/69‑1 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg > 1:923800/1‑71 (MQ=255) atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg > 1:2788814/1‑71 (MQ=255) ccAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg > 1:414684/1‑70 (MQ=255) aGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAc > 1:2891093/1‑71 (MQ=255) aGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAc > 1:1994110/1‑71 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAAc > 1:2686141/1‑70 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg < 1:1378853/71‑1 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg < 1:2644544/71‑1 (MQ=255) gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg < 1:4006075/71‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTa > 1:840960/1‑58 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGa < 1:1013544/71‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGa < 1:1167868/71‑1 (MQ=255) tGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGa < 1:87849/71‑1 (MQ=255) gCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGag > 1:2528440/1‑69 (MQ=255) gaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGa > 1:3640075/1‑60 (MQ=255) aaCACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa < 1:369782/64‑1 (MQ=255) acacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCa < 1:2265718/71‑1 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAc > 1:2014142/1‑68 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAc > 1:2958471/1‑68 (MQ=255) cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAg < 1:1776823/71‑1 (MQ=255) ctctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGa < 1:2297900/59‑1 (MQ=255) ctctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGa < 1:2157698/70‑1 (MQ=255) ctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa > 1:1776233/1‑58 (MQ=255) tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg > 1:1621795/1‑71 (MQ=255) cagcaTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCATACAt > 1:1992379/1‑64 (MQ=255) cagcaTAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAg < 1:3758644/52‑1 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAt > 1:4014513/1‑70 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:3875647/71‑1 (MQ=255) tAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc < 1:157499/71‑1 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGAc > 1:247862/1‑55 (MQ=255) aTCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg > 1:3632104/1‑71 (MQ=255) tCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa < 1:2031499/43‑1 (MQ=255) tCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGt < 1:1229802/71‑1 (MQ=255) cAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGaa > 1:1845877/1‑38 (MQ=255) cAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGa < 1:1121280/52‑1 (MQ=255) cAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg > 1:558273/1‑69 (MQ=255) aaTTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGc > 1:657242/1‑71 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACa > 1:128316/1‑53 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACa > 1:224247/1‑53 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACa > 1:831138/1‑53 (MQ=255) aaTTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTg < 1:1144405/70‑1 (MQ=255) gATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAg > 1:1307840/1‑70 (MQ=255) tACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCAt < 1:2325480/71‑1 (MQ=255) aCCCTTTGCCATCTTGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:2845442/1‑71 (MQ=255) aCCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCAcc < 1:757077/41‑1 (MQ=255) cccTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTg > 1:2314176/1‑62 (MQ=255) cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:838207/1‑70 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:1037803/1‑69 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc < 1:3842795/69‑1 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:3558424/1‑69 (MQ=255) ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc > 1:3553429/1‑69 (MQ=255) tttGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGa > 1:1288545/1‑40 (MQ=255) aTCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCgg > 1:2490935/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAt > 1:3616668/1‑45 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:3863958/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:917545/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg > 1:2700048/1‑71 (MQ=255) tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGCTTCCGGTCg > 1:1910805/1‑71 (MQ=255) gCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg < 1:3878132/69‑1 (MQ=255) cTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGt < 1:3001650/71‑1 (MQ=255) | TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT > minE/706611‑706750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |