| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 718,576 | G→A | 46.5% | intergenic (+19/‑91) | mviM → / → mviN | predicted oxidoreductase, NAD(P)‑binding Rossmann‑fold domain/predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 718,576 | 0 | G | A | 46.5% | 8.5 / 56.3 | 43 | intergenic (+19/‑91) | mviM/mviN | predicted oxidoreductase, NAD(P)‑binding Rossmann‑fold domain/predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (6/17); new base A (11/9); total (17/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGC > minE/718512‑718642 | gcAAGGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCaa < 1:3883113/71‑1 (MQ=255)gcAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCaa < 1:2487287/71‑1 (MQ=255) cAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAAt < 1:3948810/71‑1 (MQ=255) aCGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAAtt > 1:1329124/1‑70 (MQ=255) tCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCa > 1:929282/1‑70 (MQ=255) gTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATa < 1:1603089/70‑1 (MQ=255) gTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGATa > 1:629404/1‑57 (MQ=255) gACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATc < 1:4144111/70‑1 (MQ=255) aaGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAgg < 1:953578/71‑1 (MQ=255) aGATCTGGCGCGATGCGATGAGTGAAT‑AACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTa < 1:781902/71‑1 (MQ=255) gATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTa < 1:2509335/71‑1 (MQ=255) gATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTa < 1:2509874/71‑1 (MQ=255) gcgcGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAg < 1:3473962/66‑1 (MQ=255) gcgcGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTa < 1:2831576/70‑1 (MQ=255) atgcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTa < 1:378997/60‑1 (MQ=255) atgAGTGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCttt < 1:3692378/71‑1 (MQ=255) tgAGTGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCttt > 1:3687380/1‑70 (MQ=255) aGTGAATATACCCTGTAACATCTGGCGATAGCAATTCAcc < 1:365375/40‑1 (MQ=38) aGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATc < 1:169073/46‑1 (MQ=255) tGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTa < 1:3496936/55‑1 (MQ=255) tGAATAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAgcg < 1:1484121/71‑1 (MQ=255) aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACt > 1:3153307/1‑52 (MQ=255) aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCa > 1:3283597/1‑66 (MQ=255) aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCgcc > 1:3637639/1‑71 (MQ=255) aaTAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCgcc > 1:2145563/1‑71 (MQ=255) tAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAg < 1:1671724/47‑1 (MQ=255) tAAACCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCgcctg > 1:1501642/1‑71 (MQ=255) aaCCCTGTAACATCTGGCGATAGCAATTCACCATAATCCa > 1:4142805/1‑40 (MQ=255) aaCCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCgcctgcc > 1:2759564/1‑71 (MQ=255) aCCCTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTAtt > 1:1196539/1‑50 (MQ=255) ccTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTg < 1:3901170/71‑1 (MQ=255) cTGTAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGc < 1:2278125/71‑1 (MQ=255) tAACATCTGGCGGTTGCAATTCACCATAATCCAGGTAGACTATTc < 1:223754/45‑1 (MQ=255) tAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCctc > 1:3907160/1‑50 (MQ=255) tAACATCTGGCGATAGCAATTCACCATAATCCAGGTAGa > 1:2562107/1‑39 (MQ=255) cATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGt < 1:343176/71‑1 (MQ=255) cATCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTg < 1:1496364/64‑1 (MQ=255) aTCTGGCGGTAGCAATTCACCATAATCCAGGTAGAc < 1:1717195/36‑1 (MQ=255) aTCTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGtt < 1:3642004/71‑1 (MQ=255) cTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTc > 1:1209423/1‑48 (MQ=255) cTGGCGATAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGttt > 1:2830832/1‑70 (MQ=255) tGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTg > 1:2799918/1‑71 (MQ=255) gCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGc < 1:3493422/70‑1 (MQ=255) | GCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGC > minE/718512‑718642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |