| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,532 | C→T | 15.9% | N385N (AAC→AAT) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,532 | 0 | C | T | 15.9% | 158.4 / 20.2 | 63 | N385N (AAC→AAT) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (3/7); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
GATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTT > minE/775463‑775601 | gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg > 1:1728706/1‑71 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaa < 1:78570/71‑1 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGC‑‑CTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa > 1:3215267/1‑71 (MQ=255) gcgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaatc > 1:2342608/1‑69 (MQ=255) cgcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa < 1:2309365/70‑1 (MQ=255) taTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa < 1:789745/62‑1 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct < 1:3221389/71‑2 (MQ=255) ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:3253117/1‑54 (MQ=255) ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAc > 1:1775570/1‑54 (MQ=255) gTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt < 1:4015745/71‑1 (MQ=255) gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:1501705/71‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa > 1:3745480/1‑59 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:3811425/68‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg < 1:176514/71‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg < 1:3761392/71‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACtt > 1:1902029/1‑71 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACt < 1:2724325/70‑1 (MQ=255) tgAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa > 1:2941997/2‑51 (MQ=37) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGa > 1:3941952/1‑64 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:1870311/1‑71 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:2557579/1‑71 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:2456686/69‑1 (MQ=255) aGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac < 1:2019518/71‑3 (MQ=255) gCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCg < 1:2171348/71‑1 (MQ=255) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:3259805/1‑71 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGGGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:159289/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGt < 1:3101455/50‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:1317846/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt < 1:467250/71‑1 (MQ=255) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:694260/1‑71 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaaaa > 1:654740/1‑39 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat > 1:2556005/1‑71 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:2693609/1‑71 (MQ=255) ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCg < 1:2296297/39‑1 (MQ=255) ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc < 1:3572206/44‑1 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt > 1:3875256/1‑45 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:56177/1‑65 (MQ=255) ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCt < 1:3293128/71‑1 (MQ=255) tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:2414801/1‑38 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa > 1:2585758/1‑70 (MQ=255) tGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg < 1:3745558/71‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:1659914/56‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:584306/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:689095/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa < 1:2241532/71‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:3964817/1‑70 (MQ=255) aaGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:306101/1‑71 (MQ=255) ggCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:2428758/1‑71 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTga > 1:4079303/1‑44 (MQ=255) gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:3816672/1‑70 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa < 1:603036/71‑1 (MQ=255) aTCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa > 1:438164/1‑69 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:51584/1‑53 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:2789576/51‑1 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:4079056/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:2496505/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt > 1:745115/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:1537653/1‑60 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:2553595/67‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:852117/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:892116/1‑71 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTg < 1:1798323/35‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg < 1:2277803/61‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:3611050/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:757741/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2036013/71‑1 (MQ=255) cgcgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:554113/1‑49 (MQ=255) | GATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTT > minE/775463‑775601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |