| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,547 | C→T | 10.6% | T390T (ACC→ACT) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,547 | 0 | C | T | 10.6% | 193.6 / 11.2 | 66 | T390T (ACC→ACT) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (32/27); new base T (2/5); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
GTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCA > minE/775480‑775614 | gTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt < 1:4015745/71‑1 (MQ=255)gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:1501705/71‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:3811425/68‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg < 1:3761392/71‑1 (MQ=255) ttGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATg < 1:176514/71‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACtt > 1:1902029/1‑71 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACt < 1:2724325/70‑1 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGa > 1:3941952/1‑64 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:2557579/1‑71 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:1870311/1‑71 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:2456686/69‑1 (MQ=255) aGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac < 1:2019518/71‑3 (MQ=255) gCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCg < 1:2171348/71‑1 (MQ=255) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:3259805/1‑71 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGGGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:159289/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:1317846/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt < 1:467250/71‑1 (MQ=255) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:694260/1‑71 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat > 1:2556005/1‑71 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:2693609/1‑71 (MQ=255) ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc < 1:3572206/44‑1 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt > 1:3875256/1‑45 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:56177/1‑65 (MQ=255) ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCt < 1:3293128/71‑1 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa > 1:2585758/1‑70 (MQ=255) tGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg < 1:3745558/71‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:1659914/56‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:689095/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:584306/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa < 1:2241532/71‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:3964817/1‑70 (MQ=255) aaGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:306101/1‑71 (MQ=255) ggCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:2428758/1‑71 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTga > 1:4079303/1‑44 (MQ=255) gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:3816672/1‑70 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa < 1:603036/71‑1 (MQ=255) aTCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa > 1:438164/1‑69 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:51584/1‑53 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:2789576/51‑1 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:2496505/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:4079056/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt > 1:745115/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:1537653/1‑60 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:2553595/67‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:852117/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:892116/1‑71 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTg < 1:1798323/35‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg < 1:2277803/61‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:3611050/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:757741/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2036013/71‑1 (MQ=255) cgcgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:554113/1‑49 (MQ=255) gcgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTCCTGAAATGTTCAGAGTTTgg > 1:2691290/1‑71 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTgatgg > 1:1664229/1‑36 (MQ=255) gAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:443054/70‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt < 1:1861229/65‑1 (MQ=255) aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:589595/1‑70 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:1380692/1‑71 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:3461826/1‑71 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAct < 1:2900791/43‑1 (MQ=255) tgTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:3375972/69‑1 (MQ=255) gTAACCTATGAGTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa < 1:1763227/48‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg < 1:1760055/44‑1 (MQ=255) tAACCTATGACTTCGAGAGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:2277514/1‑70 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGGGCTATACTGCTGata < 1:2172875/45‑3 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:3860236/1‑36 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca < 1:942420/70‑1 (MQ=255) ccTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa > 1:1216677/1‑66 (MQ=255) | GTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCA > minE/775480‑775614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |