| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,559 | G→A | 9.9% | E394E (GAG→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,559 | 0 | G | A | 9.9% | 242.6 / 12.7 | 81 | E394E (GAG→GAA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base G (38/35); new base A (4/4); total (42/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGT > minE/775489‑775626 | aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:1870311/1‑71 (MQ=255)aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:2557579/1‑71 (MQ=255) gCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCg < 1:2171348/71‑1 (MQ=255) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:3259805/1‑71 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGGGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:159289/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:1317846/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt < 1:467250/71‑1 (MQ=255) tGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:694260/1‑71 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat > 1:2556005/1‑71 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:2693609/1‑71 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:56177/1‑65 (MQ=255) ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCt < 1:3293128/71‑1 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa > 1:2585758/1‑70 (MQ=255) tGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg < 1:3745558/71‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:1659914/56‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:584306/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:689095/1‑42 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa < 1:2241532/71‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:3964817/1‑70 (MQ=255) aaGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:306101/1‑71 (MQ=255) ggCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg > 1:2428758/1‑71 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTga > 1:4079303/1‑44 (MQ=255) gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:3816672/1‑70 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa < 1:603036/71‑1 (MQ=255) aTCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa > 1:438164/1‑69 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:51584/1‑53 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:2789576/51‑1 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:4079056/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgc > 1:2496505/1‑56 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt > 1:745115/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:1537653/1‑60 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:2553595/67‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:892116/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt > 1:852117/1‑71 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTg < 1:1798323/35‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg < 1:2277803/61‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:757741/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:3611050/71‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2036013/71‑1 (MQ=255) cgcgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:554113/1‑49 (MQ=255) gcgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTCCTGAAATGTTCAGAGTTTgg > 1:2691290/1‑71 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTgatgg > 1:1664229/1‑36 (MQ=255) gAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:443054/70‑1 (MQ=255) aaaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt < 1:1861229/65‑1 (MQ=255) aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:589595/1‑70 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:3461826/1‑71 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:1380692/1‑71 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAct < 1:2900791/43‑1 (MQ=255) tgTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:3375972/69‑1 (MQ=255) gTAACCTATGAGTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa < 1:1763227/48‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg < 1:1760055/44‑1 (MQ=255) tAACCTATGACTTCGAGAGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:2277514/1‑70 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGGGCTATACTGCTGata < 1:2172875/45‑3 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:3860236/1‑36 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca < 1:942420/70‑1 (MQ=255) ccTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa > 1:1216677/1‑66 (MQ=255) tATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:1651079/49‑1 (MQ=255) aTGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2249452/53‑1 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 1:179072/62‑1 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:3517652/70‑1 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa < 1:2779013/69‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca < 1:2323281/63‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatc > 1:2573086/1‑62 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:1024636/1‑70 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:1865233/71‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:2762455/71‑1 (MQ=255) cTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga < 1:1650604/45‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTc < 1:1373448/41‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTg > 1:3320992/1‑49 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt > 1:2879708/1‑51 (MQ=255) ttCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt < 1:595213/37‑1 (MQ=25) ttCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAAt < 1:2674273/37‑1 (MQ=25) tCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAAt > 1:3915664/1‑36 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:648959/1‑53 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:3083934/1‑57 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:2410303/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:2405560/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg < 1:3236137/70‑1 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3347349/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3995278/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3953634/1‑70 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg > 1:2338659/1‑70 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg > 1:964617/1‑70 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2778753/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:1592153/70‑1 (MQ=255) | AAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGT > minE/775489‑775626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |