| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 775,607 | C→A | 8.9% | D410E (GAC→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 775,607 | 0 | C | A | 8.9% | 221.6 / 5.1 | 67 | D410E (GAC→GAA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (31/30); new base A (3/3); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
AACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTT > minE/775538‑775677 | aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:1380692/1‑71 (MQ=255)aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:3461826/1‑71 (MQ=255) tgTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:3375972/69‑1 (MQ=255) tAACCTATGACTTCGAGAGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:2277514/1‑70 (MQ=255) aCCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca < 1:942420/70‑1 (MQ=255) ccTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa > 1:1216677/1‑66 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 1:179072/62‑1 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:3517652/70‑1 (MQ=255) tGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa < 1:2779013/69‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca < 1:2323281/63‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatc > 1:2573086/1‑62 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:1024636/1‑70 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:2762455/71‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:1865233/71‑1 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg > 1:648959/1‑53 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:3083934/1‑57 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg < 1:3236137/70‑1 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:2410303/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:2405560/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3347349/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3995278/1‑70 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg > 1:3953634/1‑70 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg > 1:964617/1‑70 (MQ=255) cGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATg > 1:2338659/1‑70 (MQ=255) gaacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTa < 1:3776972/68‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:1592153/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2778753/70‑1 (MQ=255) aacGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTa < 1:2672830/68‑1 (MQ=255) cGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcatc > 1:1855742/1‑57 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa > 1:1873766/1‑56 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCg > 1:1897193/1‑71 (MQ=255) cTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatcat > 1:2997723/1‑53 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat < 1:633284/49‑1 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat < 1:3160492/49‑1 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATg > 1:1065761/1‑67 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATg > 1:1770847/1‑67 (MQ=255) tGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCg > 1:810503/1‑70 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:309759/1‑63 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAg > 1:341330/1‑71 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAt < 1:3061178/56‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGttt > 1:136183/1‑71 (MQ=255) atggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:3669567/1‑59 (MQ=255) tggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGt < 1:1641257/71‑1 (MQ=255) tggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGt > 1:3609091/1‑71 (MQ=255) gcgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa < 1:1266873/71‑1 (MQ=255) cgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcatc > 1:2916913/1‑43 (MQ=255) cgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt < 1:1202046/69‑1 (MQ=255) gcTAAACTGCTGAAATGTTCAGAGTTTGGTGCCGCGATCATCGaaa > 1:4145209/1‑46 (MQ=255) gcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaaa < 1:648918/71‑1 (MQ=255) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:1873003/44‑1 (MQ=255) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAAt > 1:3191364/1‑54 (MQ=255) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGt < 1:331815/62‑1 (MQ=255) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGt < 1:1975351/62‑1 (MQ=255) aaaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:1282353/1‑51 (MQ=255) aaaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:1124964/1‑70 (MQ=255) aaaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:3135333/1‑70 (MQ=255) agctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGaa < 1:2597352/39‑1 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:2634726/1‑69 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:2562336/71‑1 (MQ=255) tgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:419521/66‑1 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:3545576/1‑45 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa > 1:4046172/1‑45 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:1884725/61‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:4035568/61‑1 (MQ=255) aTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAg > 1:4001833/1‑71 (MQ=255) aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGt < 1:3778614/38‑1 (MQ=255) ttCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGc < 1:3102705/69‑1 (MQ=255) ttCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGt < 1:2178723/71‑1 (MQ=255) gagTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:837131/52‑1 (MQ=255) aCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa < 1:1063899/38‑1 (MQ=255) aCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt < 1:3997334/41‑1 (MQ=255) cgcgATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgtt < 1:2387288/71‑1 (MQ=255) | AACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTT > minE/775538‑775677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |