| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 784,369 | A→G | 30.9% | N324D (AAC→GAC) | umuC → | DNA polymerase V, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 784,369 | 0 | A | G | 30.9% | 69.1 / 45.8 | 55 | N324D (AAC→GAC) | umuC | DNA polymerase V, subunit C |
| Reads supporting (aligned to +/- strand): ref base A (14/24); new base G (9/8); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGTGATGCT > minE/784301‑784439 | acccTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTaa < 1:3089012/68‑1 (MQ=255)aaCCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTaa < 1:2193072/70‑1 (MQ=255)aaCCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAAc > 1:217783/1‑71 (MQ=255) aCCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACg > 1:1700637/1‑71 (MQ=255) attaCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTaa > 1:3619366/1‑64 (MQ=255) attaCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTaa > 1:1863639/1‑64 (MQ=255) attaCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTa > 1:158054/1‑63 (MQ=255) attaCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCAATCAGGACAGCAGGGATATCATTAACgctgc < 1:3823423/70‑1 (MQ=255) aCGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTAcg > 1:1392333/1‑71 (MQ=255) aGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGATctct > 1:2674684/1‑71 (MQ=255) aGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACgctg > 1:977035/1‑58 (MQ=255) gcgcGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTg < 1:3847232/71‑1 (MQ=255) gcgcGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTg < 1:2806832/71‑1 (MQ=255) tCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACgc < 1:2338756/50‑1 (MQ=255) cGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGc > 1:947773/1‑70 (MQ=255) ggTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGcc < 1:1095354/70‑1 (MQ=255) gTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGAtct < 1:4012251/60‑1 (MQ=255) gTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGAtct < 1:1910488/60‑1 (MQ=255) gTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTAcgcg < 1:3790573/56‑1 (MQ=255) gTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTTGATGCCAt > 1:614398/1‑71 (MQ=255) tAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGATCTCTGGATg < 1:579361/66‑1 (MQ=255) aCTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTgg > 1:1474310/1‑70 (MQ=255) aCTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGc < 1:2001866/71‑1 (MQ=255) ctgACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCaa < 1:1040695/69‑1 (MQ=255) gACGCCCACTCAGGACAGCAGGGATATCATTGACGCTGCTAcgcg < 1:525397/45‑1 (MQ=255) gACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTgc < 1:1193261/53‑2 (MQ=255) cACTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATc < 1:1891998/71‑1 (MQ=255) cACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATc < 1:3225316/71‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGAtct < 1:486163/41‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTGACGCTGCTACGCGATCTCTGGATGcc < 1:2116423/50‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGAtct < 1:1938430/41‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATc < 1:1374184/69‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGt < 1:3805903/71‑1 (MQ=255) cTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGCCCATc < 1:3852367/69‑1 (MQ=255) tCAGGACAGCAGGGATATCATTAACGCTGCTACGCGAtc < 1:489993/39‑1 (MQ=255) tCAGGACAGCAGGGATATCATTAACGCTGCTACGCGAtc < 1:3614866/39‑1 (MQ=255) tCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGc < 1:2228827/48‑1 (MQ=255) ccAGGACAGCAGGGATATCATTGACGCTGCTACGCGATctct < 1:1921843/41‑1 (MQ=39) cAGGACAGCAGTGATATCATTGACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTa > 1:1844986/1‑71 (MQ=255) cAGGACAGCAGGGATATCATTGACGCTGCTACGCGAtctc > 1:3202580/1‑40 (MQ=255) cAGGACAGCAGGGATATCATTGACGCTGCTACGCGATCTCTgg > 1:1645701/1‑43 (MQ=255) cAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGAt > 1:1498681/1‑45 (MQ=255) cAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTa > 1:3110643/1‑71 (MQ=255) aGGACAGCAGGGATATCATTGACGCTGCTACGCGa > 1:3327851/1‑35 (MQ=255) aGGACAGCAGGGATATCATTGACGCTGCTACGCGATCTCTGGATGCCATCTg > 1:2233435/1‑52 (MQ=255) gACAGCAGGGATATCATTAACGCTGCTACGCGAtctc > 1:3973876/1‑37 (MQ=255) cagcagGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCaaa < 1:2822688/71‑1 (MQ=255) cagcagGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCaaa < 1:914403/71‑1 (MQ=255) tatCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCaa < 1:4066424/61‑1 (MQ=255) tatCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCaa < 1:1108923/61‑1 (MQ=255) tCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGt < 1:2680789/70‑1 (MQ=255) tCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGt < 1:3511416/70‑1 (MQ=255) tCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGt < 1:1647649/70‑1 (MQ=255) cATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCggg > 1:2134888/1‑45 (MQ=255) cATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATc > 1:3195365/1‑50 (MQ=255) aTTGACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCAt > 1:955876/1‑48 (MQ=255) aTTGACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGc > 1:1798153/1‑62 (MQ=255) aTTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCg > 1:1647227/1‑50 (MQ=255) aTTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGTGAt > 1:2075804/1‑71 (MQ=255) tAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGc > 1:3048376/1‑60 (MQ=255) aaCGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGTGATGc < 1:697689/70‑1 (MQ=255) aaCGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGTGATGCt > 1:1088046/1‑71 (MQ=255) | AACCTTATTACGGCAATAGCGCGTCGGTAAAACTGCTGACGCCCACTCAGGACAGCAGGGATATCATTAACGCTGCTACGCGATCTCTGGATGCCATCTGGCAAGCGGGCCATCGTTACCAAAAAGCGGGCGTGATGCT > minE/784301‑784439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |