| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,218 | G→A | 45.6% | L280L (CTG→CTA) | kdsA → | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,218 | 0 | G | A | 45.6% | 11.6 / 59.6 | 46 | L280L (CTG→CTA) | kdsA | 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (6/19); new base A (9/12); total (15/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01 | |||||||||||
GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCA > minE/821149‑821286 | ggCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTg > 1:3535663/1‑70 (MQ=255) gCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGa < 1:2042952/71‑1 (MQ=255) cTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGAt < 1:247021/71‑1 (MQ=255) aaCTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATa > 1:2837884/1‑69 (MQ=255) gAACGGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGc < 1:2462716/69‑1 (MQ=255) gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGc < 1:3133148/69‑1 (MQ=255) gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGc < 1:1440703/69‑1 (MQ=255) gAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGc < 1:1126486/69‑1 (MQ=255) ttCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATc < 1:108779/71‑1 (MQ=255) ttCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTaa < 1:3849632/69‑1 (MQ=255) ttCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATc > 1:2396890/1‑71 (MQ=255) aaGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCtttt < 1:3886230/69‑1 (MQ=255) aaGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTg < 1:2032651/38‑1 (MQ=255) aTTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGc < 1:1133044/42‑1 (MQ=255) aTTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg < 1:2575250/71‑1 (MQ=255) aTTGATGATCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg < 1:3253185/71‑1 (MQ=255) ttGATGATCTGGTGAAAGGTTTCGAAGAACTGGAt < 1:3056723/35‑1 (MQ=255) ttGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAg < 1:2201916/70‑1 (MQ=255) tgatgaTCTGGTGAAAGGTTTCGAAGAACTAGATAc > 1:1258018/1‑36 (MQ=255) gatgatCTGGTTAAAGGTTTCGAAGAACTAGATACCAg < 1:1694600/38‑1 (MQ=37) atgatCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCtttt > 1:1154843/1‑50 (MQ=255) atgatCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAtt < 1:3381630/71‑1 (MQ=255) atgatCTGGTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTAt > 1:3106363/1‑70 (MQ=255) tgatCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTa < 1:1448882/71‑1 (MQ=255) ggTGAAAGGTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCg < 1:3266827/68‑1 (MQ=255) aaaGGTTTCGAAGAACTGGATACCAGCAAGTAATc < 1:2562324/35‑1 (MQ=255) aaaGGTTTCGAAGAACTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCg > 1:3213534/1‑71 (MQ=255) aGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa > 1:206447/1‑56 (MQ=255) aGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGt > 1:514790/1‑70 (MQ=255) gTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTaa < 1:1384130/71‑1 (MQ=255) tttCGAATAACTGGATACCAGCAAGTAATCTTTTTTGCTTg > 1:1720918/1‑41 (MQ=255) tttCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGtt > 1:2710250/1‑68 (MQ=255) tCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGAc > 1:3190807/1‑71 (MQ=255) gaagaaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaa < 1:1915414/45‑1 (MQ=255) gaagaaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtt < 1:145530/71‑1 (MQ=255) aagaaCTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa < 1:13090/43‑1 (MQ=255) agaaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGc > 1:3970874/1‑52 (MQ=255) agaaCTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttt < 1:2691959/71‑1 (MQ=255) gaaCTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt > 1:99741/1‑71 (MQ=255) gaaCTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACttttt > 1:1849774/1‑71 (MQ=255) aaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTa < 1:3661286/48‑1 (MQ=255) aaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttttt < 1:3438667/71‑1 (MQ=255) aaCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttttt < 1:1963310/71‑1 (MQ=255) aCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTa < 1:3187481/47‑1 (MQ=255) aCTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACtttttt < 1:1297129/70‑1 (MQ=255) aCTAGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTa > 1:2646525/1‑44 (MQ=255) cTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCa < 1:2602030/71‑1 (MQ=255) tGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATa < 1:2667518/37‑1 (MQ=255) ggATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaaa > 1:3894447/1‑38 (MQ=255) ggATACCAGCAAGTAATCTTTTTTGCTTGAAAAATaaa > 1:85489/1‑38 (MQ=255) | GGCTAAACTGGAACCGTTCCTCAAGCAGATGAAAGCGATTGATGATCTGGTGAAAGGTTTCGAAGAACTGGATACCAGCAAGTAATCTTTTTTGCTTGAAAAATAAAGTATTAGCGTTCTGCGTTAAGACTTTTTTCA > minE/821149‑821286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |