| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,826 | A→T | 6.9% | intergenic (‑363/+91) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,826 | 0 | A | T | 6.9% | 163.9 / 4.2 | 58 | intergenic (‑363/+91) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (28/26); new base T (2/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGG > minE/821760‑821894 | gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:1542625/70‑1 (MQ=255) cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:3177636/70‑1 (MQ=255) cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:122937/70‑1 (MQ=255) tCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:827126/67‑1 (MQ=255) cGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGa > 1:3876598/1‑70 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:3553379/67‑1 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:3558366/67‑1 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:1534259/1‑70 (MQ=255) gTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACa < 1:3605144/71‑1 (MQ=255) tttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaa < 1:1550834/71‑1 (MQ=255) ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaa > 1:805594/1‑70 (MQ=255) ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaa > 1:2815770/1‑70 (MQ=255) ttCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaa > 1:1166661/1‑70 (MQ=255) tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa > 1:1257833/1‑62 (MQ=255) tCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa > 1:3073055/1‑70 (MQ=255) cATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaa < 1:2844917/70‑1 (MQ=255) tGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATg < 1:1786145/71‑1 (MQ=255) aGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc > 1:1938505/1‑71 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:1876050/50‑1 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAg < 1:1144989/53‑1 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGTCAAAAATGGCCGCAGGc > 1:1091721/1‑71 (MQ=255) tGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:3680061/58‑1 (MQ=255) tGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGcc > 1:2308534/1‑65 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:4142411/47‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:67543/47‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa > 1:3037820/1‑58 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa > 1:2747211/1‑58 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:67060/70‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:286879/70‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:311712/1‑71 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:612083/1‑71 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:306837/1‑71 (MQ=255) atgatgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGt < 1:940345/71‑1 (MQ=255) atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa < 1:2707590/52‑1 (MQ=255) atgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTAc < 1:2900802/71‑1 (MQ=255) cTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAAATAAAGACAAAAATGGCCGCAGGCTGTTAcccc < 1:1868178/71‑1 (MQ=255) tCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:2391684/1‑41 (MQ=255) ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc > 1:2042186/1‑71 (MQ=255) gCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa < 1:2643939/43‑1 (MQ=255) cGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCgg > 1:2433141/1‑71 (MQ=255) gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa > 1:1257041/1‑41 (MQ=255) gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc < 1:2663213/52‑1 (MQ=255) gggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCt > 1:2502319/1‑62 (MQ=255) gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGttt < 1:872903/71‑1 (MQ=255) gTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaa < 1:1555046/37‑1 (MQ=255) gCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCg > 1:3548500/1‑70 (MQ=255) cAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgc > 1:1494606/1‑71 (MQ=255) atatTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg > 1:195728/1‑70 (MQ=255) atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg > 1:688340/1‑70 (MQ=255) atatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGgcg > 1:1805901/1‑70 (MQ=255) tatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa < 1:2654123/71‑1 (MQ=255) atTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa > 1:1974344/1‑38 (MQ=255) atTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa > 1:490210/1‑38 (MQ=255) atTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa > 1:3641521/1‑70 (MQ=255) tttACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTa > 1:2595143/1‑45 (MQ=255) ttACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCggg > 1:990995/1‑64 (MQ=255) tACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCatat < 1:2571420/70‑1 (MQ=255) gtgGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCa < 1:487707/70‑1 (MQ=255) agaTAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACgg > 1:1245081/1‑69 (MQ=255) | GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGG > minE/821760‑821894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |