| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,222 | T→A | 11.7% | intergenic (‑198/+206) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,222 | 0 | T | A | 11.7% | 229.3 / 19.0 | 85 | intergenic (‑198/+206) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (41/34); new base A (4/6); total (45/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAG > minE/822153‑822288 | tGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTc > 1:2013062/1‑71 (MQ=255)tGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTc > 1:1787750/1‑71 (MQ=255) gggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:2202186/1‑71 (MQ=255) gggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:2846768/1‑71 (MQ=255) ggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa < 1:1191939/70‑1 (MQ=255) aTAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:974432/1‑68 (MQ=255) tAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATctt < 1:2146569/71‑1 (MQ=255) gcccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcac > 1:3273823/3‑71 (MQ=11) aGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcac < 1:1666970/71‑1 (MQ=255) gCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcact > 1:2919341/1‑71 (MQ=255) gCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATct < 1:3254999/66‑1 (MQ=255) cccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttcact > 1:2149067/2‑71 (MQ=255) ccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:2942580/54‑1 (MQ=255) ccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCa < 1:1734175/60‑1 (MQ=11) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATc > 1:3324744/1‑71 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATc > 1:1536436/1‑71 (MQ=255) aaCATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATccc > 1:2596323/1‑71 (MQ=255) cATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCt > 1:2083464/1‑70 (MQ=255) aTTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg > 1:3341871/1‑70 (MQ=255) aTTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg > 1:2404976/1‑70 (MQ=255) aTTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa < 1:2977040/71‑1 (MQ=255) ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa > 1:3083187/1‑70 (MQ=255) ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa > 1:2850771/1‑70 (MQ=255) ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa > 1:210782/1‑70 (MQ=255) tGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaaa > 1:1574881/1‑71 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt > 1:1760770/1‑71 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc < 1:2494901/63‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATcc < 1:3879605/63‑1 (MQ=255) gcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt > 1:3874831/1‑57 (MQ=255) gcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGt > 1:1570957/1‑71 (MQ=255) gcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGt < 1:2157772/71‑1 (MQ=255) gcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:724007/71‑1 (MQ=255) gcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:1820328/71‑1 (MQ=255) ccTCGCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:134454/1‑71 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:2779871/40‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATtttt < 1:3547786/70‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:546351/42‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttc < 1:1004604/48‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:818537/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:308203/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:3029493/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:1311797/70‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:3863499/70‑1 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcac < 1:3536497/48‑1 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:3817954/1‑35 (MQ=255) aTAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATct > 1:3427354/1‑41 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg > 1:338974/1‑68 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa < 1:2705853/70‑1 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg < 1:1584760/66‑1 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:1304268/69‑1 (MQ=255) ggCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag < 1:2909769/66‑1 (MQ=40) gCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTTAAATGTTTGc < 1:808797/56‑1 (MQ=255) cTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc > 1:2463967/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCCTCTTCACTTCATCCCTGAAATGTTTGCAATGaa > 1:3405652/1‑59 (MQ=255) ggTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACAtt > 1:121181/1‑67 (MQ=40) tgtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag < 1:664239/59‑1 (MQ=255) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGt < 1:122685/44‑1 (MQ=38) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGt < 1:2370879/44‑1 (MQ=38) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:3553029/1‑48 (MQ=38) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt > 1:741679/1‑70 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2948501/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:596503/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:677457/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag < 1:3282921/53‑1 (MQ=255) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:114633/51‑1 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3517161/1‑70 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa > 1:1324150/1‑48 (MQ=255) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:406724/67‑1 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:1844206/67‑1 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:496374/1‑69 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:1021518/1‑69 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3558902/1‑69 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3371847/1‑69 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAAt < 1:1978852/35‑1 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:3266395/41‑1 (MQ=38) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAaga > 1:4060533/1‑49 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTa < 1:1121352/52‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtt < 1:2674587/63‑1 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg > 1:362386/1‑71 (MQ=39) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag > 1:1898399/1‑46 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAg < 1:1557856/69‑1 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCCTTTc < 1:1594818/64‑1 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:1784858/1‑50 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:695950/1‑50 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:2047936/1‑50 (MQ=255) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:1576839/1‑64 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:2428215/1‑64 (MQ=40) | TGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAG > minE/822153‑822288 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |