| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,578 | C→T | 40.0% | L317L (TTG→TTA) | chaA ← | calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,578 | 0 | C | T | 40.0% | 82.7 / 126.1 | 110 | L317L (TTG→TTA) | chaA | calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base C (33/33); new base T (32/12); total (65/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
AGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACT > minE/822511‑822647 | aGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa < 1:217452/70‑1 (MQ=255) ggAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAAtt > 1:3660356/1‑71 (MQ=255) agaTATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc < 1:2656231/70‑1 (MQ=255) gaTATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGt > 1:3400490/1‑71 (MQ=255) atGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTAc > 1:4096982/1‑71 (MQ=255) tGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa < 1:3365744/62‑1 (MQ=255) tGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa < 1:1185283/62‑1 (MQ=255) cTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAg < 1:478456/71‑1 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAAtt > 1:1419439/1‑51 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTa > 1:2095910/1‑56 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGc > 1:1387844/1‑70 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGc > 1:1139130/1‑70 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAt > 1:1926745/1‑50 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAt < 1:2572609/50‑1 (MQ=255) aaGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGc > 1:11860/1‑70 (MQ=255) gagGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc > 1:2716201/1‑50 (MQ=255) gCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTCTAATTc > 1:1600900/1‑47 (MQ=38) caccaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCg < 1:3996838/71‑1 (MQ=255) accaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGt > 1:2571496/1‑71 (MQ=255) accaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGt < 1:3124788/46‑1 (MQ=255) accaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt > 1:1352479/1‑71 (MQ=255) accaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt < 1:3456725/71‑1 (MQ=255) accaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt < 1:794530/71‑1 (MQ=255) ccaTCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGt < 1:1753216/45‑1 (MQ=255) caTCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTga > 1:888136/1‑71 (MQ=255) aTCACCTCCATTTCTGGCGCACCAAGTGCAAACTGCAAtt > 1:4207/1‑40 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGTaa > 1:1588086/1‑38 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac > 1:3020230/1‑71 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAAtt > 1:2123941/1‑40 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTa > 1:3898256/1‑45 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac > 1:2055959/1‑71 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac > 1:2071435/1‑71 (MQ=255) aTCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac < 1:2124416/71‑1 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa > 1:4118969/1‑37 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa > 1:730368/1‑37 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCaa > 1:1097790/1‑37 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTc < 1:780444/40‑1 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaaa > 1:2117095/1‑55 (MQ=255) tCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacg < 1:3852067/71‑1 (MQ=255) tCACCACCA‑TTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacga < 1:3953190/71‑1 (MQ=255) caccacCATTTCTGGCGCACCAAGTGCAAACTGCAATTCg > 1:4063262/1‑40 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTaa > 1:554854/1‑35 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTaa > 1:1627243/1‑35 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTaa > 1:2528419/1‑35 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTaa > 1:583760/1‑35 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTa > 1:811359/1‑42 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTa < 1:415458/42‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacga > 1:1583360/1‑70 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac < 1:767129/68‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac > 1:1300264/1‑68 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTAccc > 1:3206573/1‑45 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATaaa > 1:1497142/1‑53 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCa < 1:2375411/57‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCa < 1:1106140/57‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCTATTAGCGTgacgac < 1:4126869/71‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTa > 1:1230178/1‑61 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacgac < 1:2430579/71‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacga > 1:2199325/1‑70 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacga < 1:1389915/70‑1 (MQ=255) accaccATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTgacga > 1:1657882/1‑70 (MQ=255) ccaccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAg < 1:3948638/53‑1 (MQ=255) caccaTTTCTGGCGCACCAAGTGCAAACTGTAATTCGt < 1:2878893/38‑1 (MQ=255) caccaTTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTAccc > 1:2360276/1‑43 (MQ=255) caccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAt > 1:3016748/1‑57 (MQ=255) caccaTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAt > 1:3026414/1‑57 (MQ=255) accaTTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAgg > 1:1657388/1‑71 (MQ=255) accaTTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAgg > 1:3290911/1‑71 (MQ=255) caTTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTgac > 1:2548111/1‑63 (MQ=255) caTTTCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTa > 1:2228705/1‑71 (MQ=255) caTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGc > 1:2882238/1‑51 (MQ=255) caTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTa > 1:1088594/1‑71 (MQ=255) caTTTCTGGAGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGc < 1:624413/58‑1 (MQ=255) aTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAAt < 1:1468485/53‑1 (MQ=255) aTTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTAc > 1:1643093/1‑71 (MQ=255) tttCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATa < 1:2868198/44‑1 (MQ=255) ttCTGGCGCACCAAGTGCAAACTGTAATTCGTTAccc > 1:3299947/1‑37 (MQ=255) tCTGGCGCACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACgg > 1:245427/1‑70 (MQ=255) tCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACgg > 1:1460061/1‑70 (MQ=255) gcgcACCAAGTGCAAACTGTAATTCGTTACCCGTCATaa < 1:753085/39‑1 (MQ=255) gcgcACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTa < 1:281041/68‑1 (MQ=255) gcACCAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGa < 1:1224586/71‑1 (MQ=255) ccAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGc < 1:2118118/38‑1 (MQ=255) ccAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGt < 1:3660327/47‑1 (MQ=255) ccAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAAt < 1:114200/71‑1 (MQ=255) cAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg > 1:690990/1‑71 (MQ=255) cAAGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg > 1:3210037/1‑71 (MQ=255) cAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATg < 1:2085316/71‑1 (MQ=255) aGTGCAAACTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGt < 1:2434698/71‑1 (MQ=255) gTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGtt < 1:753423/71‑1 (MQ=255) gTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGtt < 1:758485/71‑1 (MQ=255) gTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGtt < 1:3568121/71‑1 (MQ=255) gCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaaa > 1:2537591/1‑64 (MQ=255) gCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGaaa > 1:1455665/1‑64 (MQ=255) aaCTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTaa < 1:2329145/71‑1 (MQ=255) cTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAaca > 1:2883905/1‑71 (MQ=255) cTGTAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAaca > 1:2106271/1‑71 (MQ=255) cTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGt < 1:1420981/51‑1 (MQ=255) tGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATggct > 1:167636/1‑61 (MQ=255) tGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAacac > 1:311992/1‑71 (MQ=255) gCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACt > 1:1328739/1‑71 (MQ=255) | AGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTCTGGCGCACCAAGTGCAAACTGCAATTCGTTACCCGTCATAAAGGCAATTAGCGTGACGACAGGTACGGTAAGCGAAATGGTTGCTAACACT > minE/822511‑822647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |