| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,250 | T→C | 7.1% | T236T (ACT→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,250 | 0 | T | C | 7.1% | 200.0 / 6.2 | 70 | T236T (ACT→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/36); new base C (2/3); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
TGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA > minE/832182‑832318 | tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGa > 1:3774770/1‑71 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg < 1:3651997/69‑1 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGa < 1:703097/70‑1 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGa < 1:1584377/70‑1 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGAc > 1:4146358/1‑71 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGAc > 1:2519174/1‑71 (MQ=255) gCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGAc > 1:2070949/1‑71 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg < 1:3013797/71‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa > 1:3256040/1‑71 (MQ=255) tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt > 1:4072358/1‑65 (MQ=255) tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt > 1:3910294/1‑65 (MQ=255) aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTAc > 1:505476/1‑71 (MQ=255) aGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTAcc < 1:1728745/71‑1 (MQ=255) tCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3987790/70‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:2528593/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3622156/69‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg > 1:1656659/1‑59 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:2087521/71‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:3218110/71‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:2378867/71‑1 (MQ=255) gTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGAc < 1:714021/70‑1 (MQ=255) gTATTGCGACCTGCCGCCCGCCTCGCCCATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGAc < 1:1639320/70‑1 (MQ=255) gTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc < 1:3989238/70‑1 (MQ=255) tGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGa < 1:1191767/47‑1 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:2831502/1‑57 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTg > 1:1475862/1‑44 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgct < 1:3382371/61‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:892855/70‑1 (MQ=255) gACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:2306942/71‑1 (MQ=255) gACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:1342047/71‑1 (MQ=255) tGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc < 1:3017732/59‑1 (MQ=255) tGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:1647249/1‑70 (MQ=255) gCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:140720/1‑69 (MQ=255) ctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctg > 1:3121036/1‑51 (MQ=255) ctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctg > 1:404510/1‑51 (MQ=255) ctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGc > 1:1382133/1‑71 (MQ=255) tGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAca > 1:3500645/1‑71 (MQ=255) gCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1109659/1‑43 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAg > 1:2639592/1‑64 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatc < 1:1309794/71‑1 (MQ=255) tctcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc < 1:2407774/59‑1 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1001760/1‑38 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1706253/1‑38 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGa > 1:2961882/1‑45 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:2716643/52‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatcat > 1:2371338/1‑67 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcatcat > 1:2258489/1‑67 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg < 1:3768986/69‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg < 1:993401/69‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc > 1:360151/1‑71 (MQ=255) cAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTg < 1:3718717/71‑1 (MQ=255) atgaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg > 1:3698067/4‑71 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:650133/36‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:1644265/36‑1 (MQ=255) aaaCCTGGGGCGAGCAAACTGACGTACCGGAATctg < 1:1122517/36‑1 (MQ=255) aaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg < 1:1072517/65‑1 (MQ=255) aaCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg < 1:1831548/65‑1 (MQ=255) aCCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg < 1:428872/71‑1 (MQ=255) cTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTaaa > 1:3352082/1‑42 (MQ=255) tGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGt < 1:2706459/70‑1 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa < 1:3266878/70‑1 (MQ=255) cGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg < 1:2623916/71‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATAGcc < 1:4011224/57‑1 (MQ=38) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaaa < 1:2199315/65‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTg < 1:135780/69‑1 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGcc > 1:1066559/1‑71 (MQ=255) cAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCACCTACATCATCGCCTgggg > 1:1687978/1‑59 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:4071052/1‑51 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:1672224/1‑51 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc < 1:575698/71‑1 (MQ=255) cTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2777381/1‑68 (MQ=255) cTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2705013/1‑68 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:3235057/1‑48 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:3609393/69‑1 (MQ=255) | TGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA > minE/832182‑832318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |