Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,168 T→C 12.3% G542G (GGT→GGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1680TC12.3% 149.6 / 11.2 57G542G (GGT→GGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (30/20);  new base C (3/4);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTC  >  minE/833099‑833234
                                                                     |                                                                  
tattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTg                                                                   >  1:1451057/1‑71 (MQ=255)
  ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGc                                                                  >  1:1362712/1‑70 (MQ=255)
  ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGc                                                                  <  1:232745/70‑1 (MQ=255)
     ccATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggt                                                              <  1:2457639/70‑2 (MQ=255)
          gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                                         >  1:4020747/1‑71 (MQ=255)
          gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGaa                                                         >  1:4025038/1‑71 (MQ=255)
                 aaTTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTgg                                                  >  1:2400404/1‑71 (MQ=255)
                 aaTTTGCCGATAACGCTGATAAAACGCACTGTCGTTCGATGATTATCGTCGGTGCTGGGCTGAACCACTgg                                                  <  1:2529984/71‑1 (MQ=255)
                   tttGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGt                                                 >  1:533650/1‑70 (MQ=255)
                   tttGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTa                                                <  1:4128758/71‑1 (MQ=255)
                     tGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg                                                                 >  1:1586546/1‑52 (MQ=255)
                       ccGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCAc                                            >  1:3109312/1‑71 (MQ=255)
                        cGATAACGCTGATATAACGCACGGTCGTTCGATGATTATCGTCGGt                                                                    <  1:1647403/46‑1 (MQ=255)
                        cGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGt                                                                    <  1:1147417/46‑1 (MQ=255)
                          aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGc                                                                  >  1:2471808/1‑46 (MQ=255)
                          aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                         <  1:325252/71‑1 (MQ=255)
                                cTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                                   <  1:3213866/71‑1 (MQ=255)
                                    tAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                >  1:30764/1‑70 (MQ=255)
                                     aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                              >  1:4029251/1‑71 (MQ=255)
                                      aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGaa                                 >  1:418991/1‑67 (MQ=255)
                                      aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCCGGGCTGAACCACTGGTATCACCTCGata                                     >  1:711676/1‑63 (MQ=255)
                                       aaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATc                            <  1:1195329/71‑1 (MQ=255)
                                        aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCa                                                      >  1:4017881/1‑44 (MQ=255)
                                        aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACt                                                    >  1:2229296/1‑46 (MQ=255)
                                        aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                           >  1:2684304/1‑71 (MQ=255)
                                          gCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                         <  1:2365217/55‑1 (MQ=255)
                                           cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                        <  1:1031779/71‑1 (MQ=255)
                                             cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                      >  1:3584905/1‑71 (MQ=255)
                                              ggTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                       <  1:2747546/53‑1 (MQ=255)
                                               gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                       <  1:3022604/52‑1 (MQ=255)
                                               gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                    <  1:1479669/71‑1 (MQ=255)
                                                 cGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                  >  1:3966255/1‑71 (MQ=255)
                                                  gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATc                 <  1:1547010/69‑1 (MQ=255)
                                                  gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGTTATCACCTCGa                                       <  1:2060640/49‑1 (MQ=255)
                                                   ctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCa                <  1:3507793/70‑1 (MQ=255)
                                                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa               >  1:743291/1‑70 (MQ=255)
                                                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa               <  1:838513/70‑1 (MQ=255)
                                                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa               >  1:1957518/1‑70 (MQ=255)
                                                     cGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                              >  1:1993408/1‑55 (MQ=255)
                                                     agatgatTATCGTCGGTGCGGGGCTGAACCACTGGTa                                                >  1:2614957/2‑37 (MQ=255)
                                                      gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa               <  1:3581984/69‑1 (MQ=255)
                                                         gatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGc          >  1:957155/1‑71 (MQ=255)
                                                           ttATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGaa                                 >  1:1225912/1‑46 (MQ=255)
                                                           ttATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                   >  1:4110667/1‑60 (MQ=255)
                                                            tATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATc                            >  1:3151537/1‑50 (MQ=255)
                                                             aTCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                      >  1:3046272/1‑55 (MQ=255)
                                                             aTCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAt      >  1:2868683/1‑71 (MQ=255)
                                                                gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                        <  1:2633458/50‑1 (MQ=255)
                                                                gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                       <  1:3842512/51‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                         <  1:2505111/48‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                         <  1:1881647/48‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                         <  1:1120413/48‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                       <  1:382336/50‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                       <  1:3373450/50‑1 (MQ=255)
                                                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc  >  1:3716220/1‑71 (MQ=255)
                                                                  cgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                      >  1:598088/1‑50 (MQ=255)
                                                                  cgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                     >  1:2940499/1‑51 (MQ=255)
                                                                   ggTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                       >  1:2095725/1‑48 (MQ=255)
                                                                   ggTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                     >  1:103783/1‑50 (MQ=255)
                                                                     |                                                                  
TATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTC  >  minE/833099‑833234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: