Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,198 T→C 12.5% D552D (GAT→GAC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1980TC12.5% 169.1 / 15.2 64D552D (GAT→GACnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (28/28);  new base C (3/5);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

CTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGG  >  minE/833131‑833268
                                                                   |                                                                      
cTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATg                                                                     <  1:3213866/71‑1 (MQ=255)
    tAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                                                  >  1:30764/1‑70 (MQ=255)
     aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                                                                >  1:4029251/1‑71 (MQ=255)
      aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGaa                                                                   >  1:418991/1‑67 (MQ=255)
      aaaCGCACGGTCGTTCGATGATTATCGTCGGTGCCGGGCTGAACCACTGGTATCACCTCGata                                                                       >  1:711676/1‑63 (MQ=255)
       aaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATc                                                              <  1:1195329/71‑1 (MQ=255)
        aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                             >  1:2684304/1‑71 (MQ=255)
           cACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                          <  1:1031779/71‑1 (MQ=255)
             cGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        >  1:3584905/1‑71 (MQ=255)
               gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                      <  1:1479669/71‑1 (MQ=255)
                 cGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                    >  1:3966255/1‑71 (MQ=255)
                  gctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATc                                                   <  1:1547010/69‑1 (MQ=255)
                   ctCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCa                                                  <  1:3507793/70‑1 (MQ=255)
                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 >  1:1957518/1‑70 (MQ=255)
                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 >  1:743291/1‑70 (MQ=255)
                     cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 <  1:838513/70‑1 (MQ=255)
                     cGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                                                >  1:1993408/1‑55 (MQ=255)
                      gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                                                 <  1:3581984/69‑1 (MQ=255)
                         gatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGc                                            >  1:957155/1‑71 (MQ=255)
                           ttATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGaa                                                                   >  1:1225912/1‑46 (MQ=255)
                           ttATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                                                     >  1:4110667/1‑60 (MQ=255)
                            tATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATc                                                              >  1:3151537/1‑50 (MQ=255)
                             aTCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        >  1:3046272/1‑55 (MQ=255)
                             aTCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAt                                        >  1:2868683/1‑71 (MQ=255)
                                gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                          <  1:2633458/50‑1 (MQ=255)
                                gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:3842512/51‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                                                           <  1:1881647/48‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                                                           <  1:2505111/48‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTg                                                           <  1:1120413/48‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:382336/50‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:3373450/50‑1 (MQ=255)
                                 tcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    >  1:3716220/1‑71 (MQ=255)
                                  cgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        >  1:598088/1‑50 (MQ=255)
                                  cgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                       >  1:2940499/1‑51 (MQ=255)
                                   ggTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         >  1:2095725/1‑48 (MQ=255)
                                   ggTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                       >  1:103783/1‑50 (MQ=255)
                                      gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 <  1:2675433/53‑1 (MQ=255)
                                      gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg                                >  1:3018486/1‑70 (MQ=255)
                                       cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                      <  1:1163247/47‑1 (MQ=255)
                                       cGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                      <  1:4102981/47‑1 (MQ=255)
                                           gCTGAACCACTGGTATCACCTCGATATGAACTATCGTggg                                                         <  1:3460443/40‑2 (MQ=255)
                                           gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:539666/40‑1 (MQ=255)
                                             ttaaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTg                                  <  1:2124134/59‑1 (MQ=255)
                                              taaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATc                                                   <  1:750418/42‑1 (MQ=255)
                                               aaCCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:4112180/36‑1 (MQ=255)
                                               aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                       >  1:3871801/1‑38 (MQ=255)
                                               aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCt                             <  1:2514496/64‑1 (MQ=255)
                                               aaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaata                                               >  1:2231859/1‑44 (MQ=12)
                                                aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                     <  1:860394/71‑1 (MQ=255)
                                                    cTGGTATCACCTCGATTTGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTc                    <  1:1862111/68‑1 (MQ=255)
                                                    cTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCaga                 <  1:945602/71‑1 (MQ=255)
                                                     tGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                  <  1:648578/37‑1 (MQ=255)
                                                     tGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                  <  1:977748/37‑1 (MQ=255)
                                                     tGGTATCACCTAGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag                >  1:4014660/1‑71 (MQ=255)
                                                       gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                     >  1:2034287/1‑48 (MQ=255)
                                                       gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag                <  1:3434237/69‑1 (MQ=255)
                                                       gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCg              <  1:509547/71‑1 (MQ=255)
                                                       gTATCACCTCGATATGAACTATCGTGGTATGATCAACATGCTGAtttt                                     >  1:2822667/1‑48 (MQ=255)
                                                        tATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 <  1:3568320/35‑1 (MQ=255)
                                                            aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTgtgt                         >  1:1628008/1‑55 (MQ=255)
                                                            aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggc         <  1:315065/71‑1 (MQ=255)
                                                            aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggc         >  1:684835/1‑71 (MQ=255)
                                                              cTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    >  1:1870835/1‑42 (MQ=255)
                                                              cTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGggcgg       >  1:4045942/1‑71 (MQ=255)
                                                                cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt     >  1:3053443/1‑71 (MQ=255)
                                                                   tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCg              <  1:348373/59‑1 (MQ=255)
                                                                   tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTggg  >  1:3839502/1‑71 (MQ=255)
                                                                   |                                                                      
CTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGG  >  minE/833131‑833268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: