| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,357 | C→A | 15.9% | H605Q (CAC→CAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,357 | 0 | C | A | 15.9% | 153.0 / 10.4 | 50 | H605Q (CAC→CAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (20/22); new base A (6/2); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAG > minE/833290‑833426 | aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg < 1:2975949/71‑1 (MQ=255)aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg < 1:1671413/71‑1 (MQ=255) cGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACa < 1:2215143/70‑1 (MQ=255) cGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAct > 1:3167630/1‑71 (MQ=255) aaaCCGGCTGTCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct > 1:3302308/1‑71 (MQ=255) aaaCCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCAcctc > 1:2172934/1‑70 (MQ=255) aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct < 1:1320267/71‑1 (MQ=255) aCCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCAcctcgtt > 1:1776245/1‑68 (MQ=255) cGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTAtt < 1:152361/71‑1 (MQ=255) gCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTAttta > 1:2680760/1‑70 (MQ=255) gCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCtat < 1:1040045/70‑1 (MQ=255) ttgCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACaa < 1:2131717/69‑1 (MQ=255) cGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATaa > 1:2773946/1‑67 (MQ=255) gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACt > 1:775826/1‑71 (MQ=255) tGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTc < 1:3227087/70‑1 (MQ=255) gcctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccag > 1:229897/4‑71 (MQ=255) gcctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccag > 1:3170020/4‑71 (MQ=255) cGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagc < 1:254711/71‑1 (MQ=255) tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCtat < 1:613701/57‑1 (MQ=255) tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCtat < 1:2309997/57‑1 (MQ=255) tGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTAt > 1:597055/1‑49 (MQ=255) tGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTcca > 1:3372621/1‑65 (MQ=255) tGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGt > 1:1590333/1‑71 (MQ=255) cccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCa < 1:184800/58‑1 (MQ=255) ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCta > 1:188770/1‑51 (MQ=255) attGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTa > 1:999774/2‑44 (MQ=255) ttGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCtat < 1:832556/50‑1 (MQ=255) tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct > 1:2874469/1‑40 (MQ=255) tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct > 1:2879497/1‑40 (MQ=255) tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAc > 1:2821326/1‑36 (MQ=255) gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCtat > 1:22360/1‑48 (MQ=255) gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGtt < 1:1186222/70‑1 (MQ=255) gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTa > 1:3239879/1‑71 (MQ=255) gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa > 1:617901/1‑71 (MQ=255) gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa > 1:2589941/1‑71 (MQ=255) gCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGt < 1:1024171/56‑1 (MQ=255) cGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGt < 1:3222984/71‑1 (MQ=255) cGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGt < 1:1574199/71‑1 (MQ=255) gTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTc < 1:2144655/71‑1 (MQ=255) cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGt > 1:2523068/1‑51 (MQ=255) cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGt > 1:685868/1‑51 (MQ=255) gcgcGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggc < 1:3085662/71‑1 (MQ=255) cgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc < 1:529854/45‑1 (MQ=255) cgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTATGaaaaag > 1:2353032/1‑60 (MQ=255) cgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTATGaaaaag > 1:1769060/1‑60 (MQ=255) gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcgg < 1:1408708/71‑1 (MQ=255) tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa < 1:2789547/55‑1 (MQ=255) tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcgg > 1:4022660/1‑68 (MQ=255) tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcg < 1:593061/64‑1 (MQ=255) tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag < 1:3654739/71‑1 (MQ=255) cacaTGAACAGCACTTCTTATTTCTATAACCACTccagcc < 1:1225315/40‑1 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTcc > 1:4035666/1‑35 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTcc > 1:1666908/1‑35 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAagag < 1:3111514/71‑1 (MQ=255) | AACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAG > minE/833290‑833426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |