| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,942 | T→C | 13.3% | T800T (ACT→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,942 | 0 | T | C | 13.3% | 193.5 / 20.3 | 75 | T800T (ACT→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (27/38); new base C (7/3); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
CGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACT > minE/833872‑834012 | cGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACt > 1:1708531/1‑71 (MQ=255) gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg < 1:1005759/71‑1 (MQ=255) cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg > 1:3671449/1‑70 (MQ=255) aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:3153370/71‑1 (MQ=255) ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg < 1:2045973/68‑1 (MQ=255) cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:231947/71‑1 (MQ=255) cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:3924447/71‑1 (MQ=255) gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATg > 1:2572356/1‑71 (MQ=255) ttctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATcc > 1:246435/4‑71 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:2866848/60‑1 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:3081313/60‑1 (MQ=255) tCCGACATCATTTTGCCGACGGCGACCTAGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt > 1:219958/1‑71 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg > 1:3315013/1‑56 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa > 1:2235529/1‑58 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:3207692/70‑1 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:2066672/70‑1 (MQ=255) tatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTc < 1:177040/70‑1 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata > 1:1993174/1‑55 (MQ=255) taatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc < 1:2815987/59‑1 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:491623/1‑71 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:939747/1‑71 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:2515037/1‑71 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc < 1:1369638/59‑1 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt > 1:183438/1‑61 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt > 1:1647330/1‑61 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc > 1:3112857/1‑69 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAc > 1:3445131/1‑69 (MQ=255) ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCg > 1:3592799/1‑71 (MQ=255) tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa > 1:219025/1‑48 (MQ=255) tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa > 1:1153484/1‑62 (MQ=255) ttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc > 1:1755971/1‑56 (MQ=255) ggCGACCTGGTACGAAAAAGACGACATGAATACTTCgg < 1:3427571/38‑1 (MQ=255) ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg < 1:1414830/49‑1 (MQ=255) gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGttt < 1:4143440/51‑1 (MQ=255) gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGttt < 1:2670576/51‑1 (MQ=255) gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGttt < 1:79757/51‑1 (MQ=255) aCCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGt < 1:1994593/71‑1 (MQ=255) ccTGGTACGAAAAACACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTc < 1:336103/71‑1 (MQ=255) cTGGTACGAAAAAGACGACATGAATACTTCGGata > 1:466743/1‑35 (MQ=255) tGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa > 1:2608466/1‑71 (MQ=255) tGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa < 1:1483065/46‑1 (MQ=255) ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGAt > 1:3901467/1‑71 (MQ=255) ggTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGAt > 1:1187164/1‑71 (MQ=255) ggTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTAtt > 1:2699583/1‑47 (MQ=255) gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGAt < 1:2583304/70‑1 (MQ=255) gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATc > 1:1557574/1‑71 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:1155464/69‑1 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:170601/69‑1 (MQ=255) tACTAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc < 1:4056115/46‑1 (MQ=255) tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc < 1:3990826/46‑1 (MQ=255) tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATcc > 1:3615375/1‑71 (MQ=255) cGAAAAAGACGACATGAATACTTCGGATATGCATCCGt < 1:2190695/38‑1 (MQ=255) cGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa < 1:3783259/41‑1 (MQ=255) aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt > 1:419502/1‑69 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGtt < 1:3207695/36‑1 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:2617856/63‑1 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGttt > 1:1886943/1‑36 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg > 1:3654128/1‑71 (MQ=255) aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg > 1:1927852/1‑71 (MQ=255) aaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTgg < 1:3488708/71‑1 (MQ=255) aGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTggg < 1:3506043/71‑1 (MQ=255) acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCg < 1:1667585/71‑1 (MQ=255) gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa > 1:831315/1‑64 (MQ=255) gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa > 1:3700578/1‑64 (MQ=255) aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGaaaa < 1:1804756/70‑1 (MQ=255) aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAg > 1:2528268/1‑71 (MQ=255) aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCg < 1:3236200/48‑1 (MQ=255) aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc > 1:3514075/1‑71 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg < 1:2408507/52‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTggg < 1:1392849/59‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:2320193/70‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:2382236/70‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc > 1:2682221/1‑70 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCg < 1:4019447/71‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCg < 1:3559300/71‑1 (MQ=255) cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGAc < 1:3628093/71‑1 (MQ=255) ttCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACt > 1:2570644/1‑71 (MQ=255) | CGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACT > minE/833872‑834012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |