| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,993 | C→G | 9.7% | A817A (GCC→GCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,993 | 0 | C | G | 9.7% | 142.4 / 6.0 | 52 | A817A (GCC→GCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (20/26); new base G (3/2); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
AAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGC > minE/833925‑834056 | aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg > 1:3654128/1‑71 (MQ=255)aaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTg > 1:1927852/1‑71 (MQ=255) aaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTgg < 1:3488708/71‑1 (MQ=255) aGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTggg < 1:3506043/71‑1 (MQ=255) acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCg < 1:1667585/71‑1 (MQ=255) gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa > 1:3700578/1‑64 (MQ=255) gAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGaa > 1:831315/1‑64 (MQ=255) aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGaaaa < 1:1804756/70‑1 (MQ=255) aaTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAg > 1:2528268/1‑71 (MQ=255) aTACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc > 1:3514075/1‑71 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTggg < 1:1392849/59‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc > 1:2682221/1‑70 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:2320193/70‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:2382236/70‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCg < 1:4019447/71‑1 (MQ=255) tACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCg < 1:3559300/71‑1 (MQ=255) cTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGAc < 1:3628093/71‑1 (MQ=255) ttCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACt > 1:2570644/1‑71 (MQ=255) cGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTgg > 1:712848/1‑71 (MQ=255) ggATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTgg > 1:2041163/1‑52 (MQ=38) ggATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTgg > 1:2046069/1‑52 (MQ=38) atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGaa < 1:3058670/71‑1 (MQ=255) tGCATCCGTTTATTCACCCGCTTTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATc > 1:848939/1‑71 (MQ=255) tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:3124740/59‑1 (MQ=255) tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGc < 1:647183/59‑1 (MQ=255) gCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATc < 1:111319/70‑1 (MQ=255) cGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAAt > 1:909006/1‑64 (MQ=255) cGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGa > 1:3973903/1‑55 (MQ=255) tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGaa < 1:2501540/47‑1 (MQ=255) tttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGc < 1:1345412/71‑1 (MQ=255) ttATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGc > 1:271761/1‑70 (MQ=255) tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGcc < 1:1142873/70‑1 (MQ=255) aTTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCAt > 1:1229310/1‑71 (MQ=255) aCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAAt > 1:33336/1‑55 (MQ=255) ccGCTTTCTGCGGCGGTCGATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACaa < 1:2234632/59‑1 (MQ=255) gCTGTCTGCGGCGGTCGATCCTGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:3680703/71‑1 (MQ=255) gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:1948288/71‑1 (MQ=255) gCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:1511280/71‑1 (MQ=255) cTGGCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:128519/70‑1 (MQ=255) tGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGaaa > 1:3088100/1‑71 (MQ=255) gTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAAt > 1:2487114/1‑71 (MQ=255) tGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaa > 1:1397052/1‑63 (MQ=255) tGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct > 1:2154032/1‑71 (MQ=255) cggcggTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc > 1:3339693/1‑71 (MQ=255) gcggTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACa > 1:2138365/1‑46 (MQ=255) gcggTCGATCCGGCATGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa < 1:1461580/71‑1 (MQ=255) aTCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGc < 1:3592606/71‑1 (MQ=255) gCCTGGGAAGCGAAACGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:2191106/49‑1 (MQ=255) gCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:2545953/49‑1 (MQ=255) gCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCgaaga < 1:1681103/49‑1 (MQ=255) gCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCg < 1:2621834/58‑1 (MQ=255) gCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCg < 1:950822/58‑1 (MQ=255) | AAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGC > minE/833925‑834056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |