| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,620 | T→A | 6.5% | G1026G (GGT→GGA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,620 | 0 | T | A | 6.5% | 166.6 / 2.7 | 61 | G1026G (GGT→GGA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/35); new base A (2/2); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACC > minE/834550‑834690 | aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt < 1:2348408/71‑1 (MQ=255) gCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt > 1:2913607/1‑70 (MQ=255) ccGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATa < 1:272517/71‑1 (MQ=255) ccGCGCAAAATTATCTCCAGCCCGACATGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa < 1:2163643/71‑1 (MQ=255) aTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGt > 1:1232942/1‑71 (MQ=255) aTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGt < 1:2594773/71‑1 (MQ=255) ttATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt > 1:731391/1‑71 (MQ=255) tcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa < 1:2579058/56‑1 (MQ=255) gCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCa < 1:593070/57‑1 (MQ=255) ccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa > 1:2796898/1‑50 (MQ=255) ccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGAt < 1:1347082/71‑1 (MQ=255) ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCTGTTa > 1:3585625/1‑46 (MQ=255) ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTa > 1:2234482/1‑46 (MQ=255) ctggtctggtCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCAt > 1:307503/1‑71 (MQ=255) tggtctggtcTGGAAGATGAACACGTTTCTTACAACGCCGGTTa < 1:1593722/44‑1 (MQ=255) ggtctggtctGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTc < 1:1974179/54‑1 (MQ=255) gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa > 1:2797452/1‑67 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCAt > 1:3385649/1‑67 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:4145513/1‑71 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:2938135/1‑71 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:2624022/1‑71 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg < 1:2396592/71‑1 (MQ=255) ggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:2760870/61‑1 (MQ=255) ggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc > 1:2496529/1‑61 (MQ=255) tctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt < 1:1017930/46‑1 (MQ=255) tctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt < 1:186904/46‑1 (MQ=255) tggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCa > 1:189373/1‑46 (MQ=255) gAAGATTAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:2752234/1‑71 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:2804917/55‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct < 1:1371975/70‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct < 1:233608/68‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg < 1:2570987/58‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg < 1:735766/58‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg < 1:981661/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATccc < 1:3406873/53‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:2564465/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:147719/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:2816892/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:3062528/71‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg < 1:1138669/54‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGATACACCAACGTTCACGAGCTGATCCCATg < 1:3252305/54‑1 (MQ=255) tGAACACGTTTCTTACAACCCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc > 1:3910468/1‑71 (MQ=255) gAACACGTTTCTTACAACGCCGGTTACACCCACGTTCACGAGCTGATCCCAt > 1:3620640/1‑52 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt > 1:1312727/1‑71 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt < 1:3338650/71‑1 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1033494/1‑71 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1602219/1‑71 (MQ=255) tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct < 1:1552652/50‑1 (MQ=255) tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:37391/56‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:3837979/1‑52 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt < 1:3511710/69‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt < 1:2808065/69‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa < 1:2108158/70‑1 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg > 1:430498/1‑71 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg > 1:2179343/1‑71 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:3689754/1‑62 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:172358/1‑62 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg < 1:3209890/71‑1 (MQ=255) cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa < 1:1037343/58‑1 (MQ=255) cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc < 1:226872/71‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACg < 1:833036/38‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc < 1:441215/71‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc < 1:454433/71‑1 (MQ=255) ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc < 1:2115458/71‑1 (MQ=255) ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATAAGGATCAcc < 1:2147240/71‑1 (MQ=255) | AGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACC > minE/834550‑834690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |