| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,641 | G→A | 8.1% | L1033L (CTG→CTA) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,641 | 0 | G | A | 8.1% | 232.5 / 8.1 | 74 | L1033L (CTG→CTA) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (28/40); new base A (4/2); total (32/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
CCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTC > minE/834573‑834707 | ccGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGAt < 1:1347082/71‑1 (MQ=255) ctggtctggtCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCAt > 1:307503/1‑71 (MQ=255) gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa > 1:2797452/1‑67 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCAt > 1:3385649/1‑67 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:2624022/1‑71 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:2938135/1‑71 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg < 1:2396592/71‑1 (MQ=255) tctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:4145513/1‑71 (MQ=255) ggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc > 1:2496529/1‑61 (MQ=255) ggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:2760870/61‑1 (MQ=255) gAAGATTAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:2752234/1‑71 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:2804917/55‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct < 1:1371975/70‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct < 1:233608/68‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg < 1:2570987/58‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg < 1:735766/58‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg < 1:981661/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATccc < 1:3406873/53‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:3062528/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:2816892/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:2564465/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:147719/71‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg < 1:1138669/54‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGATACACCAACGTTCACGAGCTGATCCCATg < 1:3252305/54‑1 (MQ=255) tGAACACGTTTCTTACAACCCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc > 1:3910468/1‑71 (MQ=255) gAACACGTTTCTTACAACGCCGGTTACACCCACGTTCACGAGCTGATCCCAt > 1:3620640/1‑52 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt < 1:3338650/71‑1 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt > 1:1312727/1‑71 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1033494/1‑71 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1602219/1‑71 (MQ=255) tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct < 1:1552652/50‑1 (MQ=255) tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg < 1:37391/56‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:3837979/1‑52 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt < 1:3511710/69‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt < 1:2808065/69‑1 (MQ=255) aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa < 1:2108158/70‑1 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg > 1:430498/1‑71 (MQ=255) aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg > 1:2179343/1‑71 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:172358/1‑62 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:3689754/1‑62 (MQ=255) aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg < 1:3209890/71‑1 (MQ=255) cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa < 1:1037343/58‑1 (MQ=255) cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc < 1:226872/71‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACg < 1:833036/38‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc < 1:454433/71‑1 (MQ=255) gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc < 1:441215/71‑1 (MQ=255) ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc < 1:2115458/71‑1 (MQ=255) ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATAAGGATCAcc < 1:2147240/71‑1 (MQ=255) atACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatc > 1:2216851/2‑69 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa > 1:2353341/1‑50 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa > 1:27537/1‑50 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCa > 1:631924/1‑68 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc > 1:4120813/1‑69 (MQ=255) tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc > 1:786533/1‑69 (MQ=255) acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGa < 1:3736207/64‑1 (MQ=255) acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg > 1:272026/1‑71 (MQ=255) acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg > 1:670615/1‑71 (MQ=255) cacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg < 1:504026/62‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGGAACTGTATCAGGATCACCAGTg < 1:1025442/71‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa < 1:3904627/50‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg < 1:3576880/71‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg < 1:3505227/71‑1 (MQ=255) acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg > 1:3068521/1‑71 (MQ=255) ccAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg < 1:3910876/70‑1 (MQ=255) aaCGTTGACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa > 1:538444/1‑70 (MQ=255) aaCGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGAt > 1:1990523/1‑71 (MQ=255) cGTTCACGAGCTGATCCCATGGGGTACGCTCTCTGGTCGGCAGc < 1:3624101/44‑1 (MQ=255) ttCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg < 1:1730817/64‑1 (MQ=255) ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:314352/1‑44 (MQ=255) ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:2833849/1‑44 (MQ=255) tCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg < 1:3558390/63‑1 (MQ=255) tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa > 1:2609143/1‑48 (MQ=255) gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa < 1:1271156/56‑1 (MQ=255) aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTc < 1:2607396/71‑1 (MQ=255) | CCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTC > minE/834573‑834707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |