Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,659 C→G 10.9% L1039L (CTC→CTG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6590CG10.9% 198.7 / 13.5 73L1039L (CTC→CTGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (27/38);  new base G (5/3);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

GAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTA  >  minE/834591‑834727
                                                                    |                                                                    
gAAGATTAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                                                                    >  1:2752234/1‑71 (MQ=255)
gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctct                                                                     <  1:1371975/70‑1 (MQ=255)
  aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                  <  1:981661/71‑1 (MQ=255)
   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                 <  1:3062528/71‑1 (MQ=255)
   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                 <  1:2816892/71‑1 (MQ=255)
   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                 <  1:2564465/71‑1 (MQ=255)
   gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                 <  1:147719/71‑1 (MQ=255)
     tGAACACGTTTCTTACAACCCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc                                                               >  1:3910468/1‑71 (MQ=255)
       aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                             <  1:3338650/71‑1 (MQ=255)
       aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                             >  1:1312727/1‑71 (MQ=255)
               tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                     >  1:1033494/1‑71 (MQ=255)
               tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                     >  1:1602219/1‑71 (MQ=255)
                  tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                 <  1:37391/56‑1 (MQ=255)
                   aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                                                                    >  1:3837979/1‑52 (MQ=255)
                   aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                   <  1:2808065/69‑1 (MQ=255)
                   aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                   <  1:3511710/69‑1 (MQ=255)
                   aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                  <  1:2108158/70‑1 (MQ=255)
                      aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                              >  1:2179343/1‑71 (MQ=255)
                      aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                              >  1:430498/1‑71 (MQ=255)
                      aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                       >  1:172358/1‑62 (MQ=255)
                      aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                       >  1:3689754/1‑62 (MQ=255)
                      aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg                                              <  1:3209890/71‑1 (MQ=255)
                          cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                       <  1:1037343/58‑1 (MQ=255)
                          cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc                                          <  1:226872/71‑1 (MQ=255)
                            gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                        <  1:441215/71‑1 (MQ=255)
                            gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                        <  1:454433/71‑1 (MQ=255)
                             ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                       <  1:2115458/71‑1 (MQ=255)
                             ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATAAGGATCAcc                                       <  1:2147240/71‑1 (MQ=255)
                             atACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatc                                       >  1:2216851/2‑69 (MQ=255)
                              tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                           >  1:2353341/1‑50 (MQ=255)
                              tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                           >  1:27537/1‑50 (MQ=255)
                              tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCa                                         >  1:631924/1‑68 (MQ=255)
                              tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                        >  1:4120813/1‑69 (MQ=255)
                              tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                        >  1:786533/1‑69 (MQ=255)
                               acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGa                                            <  1:3736207/64‑1 (MQ=255)
                               acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                     >  1:272026/1‑71 (MQ=255)
                               acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                     >  1:670615/1‑71 (MQ=255)
                                cacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                             <  1:504026/62‑1 (MQ=255)
                                 acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGGAACTGTATCAGGATCACCAGTg                                   <  1:1025442/71‑1 (MQ=255)
                                 acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa                                                        <  1:3904627/50‑1 (MQ=255)
                                 acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                   <  1:3505227/71‑1 (MQ=255)
                                 acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                   >  1:3068521/1‑71 (MQ=255)
                                 acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                   <  1:3576880/71‑1 (MQ=255)
                                  ccAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                   <  1:3910876/70‑1 (MQ=255)
                                    aaCGTTGACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                 >  1:538444/1‑70 (MQ=255)
                                    aaCGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGAt                                >  1:1990523/1‑71 (MQ=255)
                                      cGTTCACGAGCTGATCCCATGGGGTACGCTCTCTGGTCGGCAGc                                                         <  1:3624101/44‑1 (MQ=255)
                                        ttCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                   <  1:1730817/64‑1 (MQ=255)
                                        ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                       >  1:314352/1‑44 (MQ=255)
                                        ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                       >  1:2833849/1‑44 (MQ=255)
                                         tCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                   <  1:3558390/63‑1 (MQ=255)
                                         tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                  >  1:2609143/1‑48 (MQ=255)
                                             gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                      <  1:1271156/56‑1 (MQ=255)
                                              aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTc                      <  1:2607396/71‑1 (MQ=255)
                                                   aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                 <  1:2740569/71‑1 (MQ=255)
                                                    tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                 >  1:664217/1‑70 (MQ=255)
                                                     cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                            >  1:1653097/1‑58 (MQ=255)
                                                     cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                            >  1:2933068/1‑58 (MQ=255)
                                                      ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa               <  1:115876/70‑1 (MQ=255)
                                                       cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc             >  1:3786913/1‑71 (MQ=255)
                                                        aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                   <  1:3582098/48‑1 (MQ=255)
                                                        aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                            <  1:760596/55‑1 (MQ=255)
                                                        aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                            <  1:2906819/55‑1 (MQ=255)
                                                        aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                        >  1:621877/1‑59 (MQ=255)
                                                           gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg          <  1:3169164/70‑1 (MQ=255)
                                                             gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                     <  1:1333000/57‑1 (MQ=255)
                                                              tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                       <  1:3442447/38‑1 (MQ=255)
                                                              tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                            <  1:624552/49‑1 (MQ=255)
                                                              tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc         <  1:296592/68‑1 (MQ=255)
                                                                cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt    >  1:3038211/1‑71 (MQ=255)
                                                                cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt    >  1:2927155/1‑71 (MQ=255)
                                                                cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt    >  1:3431161/1‑71 (MQ=255)
                                                                 gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct    >  1:376794/1‑68 (MQ=255)
                                                                 gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt   <  1:2395589/71‑1 (MQ=255)
                                                                 gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                 >  1:2888102/1‑41 (MQ=255)
                                                                 gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt   <  1:1204715/71‑1 (MQ=255)
                                                                  ctctctGGTCGTCATCAACTGTATCAGGATCACCAGTGGTTGCGTGATTTCGGTGAAAGCCTGCTGGtt    >  1:3081832/1‑69 (MQ=255)
                                                                  ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa  >  1:3618344/1‑71 (MQ=255)
                                                                  ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa  >  1:3258132/1‑71 (MQ=255)
                                                                  ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa  >  1:1420501/1‑71 (MQ=255)
                                                                  ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa  <  1:171778/71‑1 (MQ=255)
                                                                  ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTCGttt   >  1:1739815/1‑70 (MQ=255)
                                                                    |                                                                    
GAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTA  >  minE/834591‑834727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: