Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,662 T→G 11.0% S1040S (TCT→TCG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6620TG11.0% 162.7 / 20.0 73S1040S (TCT→TCGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (27/38);  new base G (5/3);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

AGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834593‑834732
                                                                     |                                                                      
aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                                                                       <  1:981661/71‑1 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:3062528/71‑1 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:2816892/71‑1 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:2564465/71‑1 (MQ=255)
 gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:147719/71‑1 (MQ=255)
   tGAACACGTTTCTTACAACCCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtc                                                                    >  1:3910468/1‑71 (MQ=255)
     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  <  1:3338650/71‑1 (MQ=255)
     aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                                                                  >  1:1312727/1‑71 (MQ=255)
             tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:1033494/1‑71 (MQ=255)
             tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                          >  1:1602219/1‑71 (MQ=255)
                tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTgg                                                                      <  1:37391/56‑1 (MQ=255)
                 aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                        <  1:2808065/69‑1 (MQ=255)
                 aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                        <  1:3511710/69‑1 (MQ=255)
                 aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                       <  1:2108158/70‑1 (MQ=255)
                    aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                   >  1:430498/1‑71 (MQ=255)
                    aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                   >  1:2179343/1‑71 (MQ=255)
                    aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            >  1:172358/1‑62 (MQ=255)
                    aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            >  1:3689754/1‑62 (MQ=255)
                    aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg                                                   <  1:3209890/71‑1 (MQ=255)
                        cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            <  1:1037343/58‑1 (MQ=255)
                        cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc                                               <  1:226872/71‑1 (MQ=255)
                          gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             <  1:454433/71‑1 (MQ=255)
                          gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             <  1:441215/71‑1 (MQ=255)
                           ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:2115458/71‑1 (MQ=255)
                           ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATAAGGATCAcc                                            <  1:2147240/71‑1 (MQ=255)
                           atACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatc                                            >  1:2216851/2‑69 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:2353341/1‑50 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCa                                                                >  1:27537/1‑50 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCa                                              >  1:631924/1‑68 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             >  1:4120813/1‑69 (MQ=255)
                            tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                             >  1:786533/1‑69 (MQ=255)
                             acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGa                                                 <  1:3736207/64‑1 (MQ=255)
                             acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                          >  1:670615/1‑71 (MQ=255)
                             acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                          >  1:272026/1‑71 (MQ=255)
                              cacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                  <  1:504026/62‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGGAACTGTATCAGGATCACCAGTg                                        <  1:1025442/71‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCa                                                             <  1:3904627/50‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3576880/71‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3505227/71‑1 (MQ=255)
                               acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        >  1:3068521/1‑71 (MQ=255)
                                ccAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3910876/70‑1 (MQ=255)
                                  aaCGTTGACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:538444/1‑70 (MQ=255)
                                  aaCGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGAt                                     >  1:1990523/1‑71 (MQ=255)
                                    cGTTCACGAGCTGATCCCATGGGGTACGCTCTCTGGTCGGCAGc                                                              <  1:3624101/44‑1 (MQ=255)
                                      ttCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                        <  1:1730817/64‑1 (MQ=255)
                                      ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            >  1:314352/1‑44 (MQ=255)
                                      ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                            >  1:2833849/1‑44 (MQ=255)
                                       tCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                        <  1:3558390/63‑1 (MQ=255)
                                       tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                       >  1:2609143/1‑48 (MQ=255)
                                           gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                           <  1:1271156/56‑1 (MQ=255)
                                            aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTc                           <  1:2607396/71‑1 (MQ=255)
                                                 aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      <  1:2740569/71‑1 (MQ=255)
                                                  tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                      >  1:664217/1‑70 (MQ=255)
                                                   cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:1653097/1‑58 (MQ=255)
                                                   cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 >  1:2933068/1‑58 (MQ=255)
                                                    ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                    <  1:115876/70‑1 (MQ=255)
                                                     cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                  >  1:3786913/1‑71 (MQ=255)
                                                      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:3582098/48‑1 (MQ=255)
                                                      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 <  1:2906819/55‑1 (MQ=255)
                                                      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 <  1:760596/55‑1 (MQ=255)
                                                      aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                             >  1:621877/1‑59 (MQ=255)
                                                         gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg               <  1:3169164/70‑1 (MQ=255)
                                                           gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                          <  1:1333000/57‑1 (MQ=255)
                                                            tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                            <  1:3442447/38‑1 (MQ=255)
                                                            tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                 <  1:624552/49‑1 (MQ=255)
                                                            tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc              <  1:296592/68‑1 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:3431161/1‑71 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:2927155/1‑71 (MQ=255)
                                                              cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:3038211/1‑71 (MQ=255)
                                                               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct         >  1:376794/1‑68 (MQ=255)
                                                               gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt        <  1:2395589/71‑1 (MQ=255)
                                                               gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                      >  1:2888102/1‑41 (MQ=255)
                                                               gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt        <  1:1204715/71‑1 (MQ=255)
                                                                ctctctGGTCGTCATCAACTGTATCAGGATCACCAGTGGTTGCGTGATTTCGGTGAAAGCCTGCTGGtt         >  1:3081832/1‑69 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       >  1:3618344/1‑71 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       <  1:171778/71‑1 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       >  1:3258132/1‑71 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa       >  1:1420501/1‑71 (MQ=255)
                                                                ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTCGttt        >  1:1739815/1‑70 (MQ=255)
                                                                   tctGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                        <  1:1971055/35‑1 (MQ=255)
                                                                   tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                   <  1:1841674/56‑1 (MQ=255)
                                                                   tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                   <  1:3556461/56‑1 (MQ=255)
                                                                   tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt      <  1:1090916/69‑1 (MQ=255)
                                                                   tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt      <  1:2968038/69‑1 (MQ=255)
                                                                    ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt   >  1:1377955/1‑71 (MQ=255)
                                                                     tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc     <  1:3732729/68‑1 (MQ=255)
                                                                     tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg    <  1:1502069/69‑1 (MQ=255)
                                                                     tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc  >  1:492068/1‑71 (MQ=255)
                                                                     |                                                                      
AGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTC  >  minE/834593‑834732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: