Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,674 A→G 9.9% Q1044Q (CAA→CAG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6740AG 9.9% 223.8 / 14.3 81Q1044Q (CAA→CAGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (31/42);  new base G (5/3);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

TCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACA  >  minE/834606‑834744
                                                                    |                                                                      
tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                                      >  1:1033494/1‑71 (MQ=255)
tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                                                                      >  1:1602219/1‑71 (MQ=255)
    aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                                    <  1:2808065/69‑1 (MQ=255)
    aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGt                                                                    <  1:3511710/69‑1 (MQ=255)
    aCAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                                   <  1:2108158/70‑1 (MQ=255)
       aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                               >  1:2179343/1‑71 (MQ=255)
       aCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAg                                                               >  1:430498/1‑71 (MQ=255)
       aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        >  1:172358/1‑62 (MQ=255)
       aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        >  1:3689754/1‑62 (MQ=255)
       aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg                                                               <  1:3209890/71‑1 (MQ=255)
           cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        <  1:1037343/58‑1 (MQ=255)
           cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc                                                           <  1:226872/71‑1 (MQ=255)
             gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                                         <  1:441215/71‑1 (MQ=255)
             gTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                                         <  1:454433/71‑1 (MQ=255)
              ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                                        <  1:2115458/71‑1 (MQ=255)
              ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATAAGGATCAcc                                                        <  1:2147240/71‑1 (MQ=255)
              atACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCatc                                                        >  1:2216851/2‑69 (MQ=255)
               tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCa                                                          >  1:631924/1‑68 (MQ=255)
               tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                                         >  1:4120813/1‑69 (MQ=255)
               tACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAc                                                         >  1:786533/1‑69 (MQ=255)
                acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGa                                                             <  1:3736207/64‑1 (MQ=255)
                acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                      >  1:272026/1‑71 (MQ=255)
                acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                      >  1:670615/1‑71 (MQ=255)
                 cacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg                                                              <  1:504026/62‑1 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGGAACTGTATCAGGATCACCAGTg                                                    <  1:1025442/71‑1 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:3505227/71‑1 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    >  1:3068521/1‑71 (MQ=255)
                  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:3576880/71‑1 (MQ=255)
                   ccAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:3910876/70‑1 (MQ=255)
                     aaCGTTGACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:538444/1‑70 (MQ=255)
                     aaCGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGAt                                                 >  1:1990523/1‑71 (MQ=255)
                         ttCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                                    <  1:1730817/64‑1 (MQ=255)
                         ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        >  1:314352/1‑44 (MQ=255)
                         ttCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                                                                        >  1:2833849/1‑44 (MQ=255)
                          tCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                                    <  1:3558390/63‑1 (MQ=255)
                          tCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTa                                                                   >  1:2609143/1‑48 (MQ=255)
                              gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                                       <  1:1271156/56‑1 (MQ=255)
                               aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTc                                       <  1:2607396/71‑1 (MQ=255)
                                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                  <  1:2740569/71‑1 (MQ=255)
                                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                  >  1:664217/1‑70 (MQ=255)
                                      cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             >  1:2933068/1‑58 (MQ=255)
                                      cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             >  1:1653097/1‑58 (MQ=255)
                                       ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                <  1:115876/70‑1 (MQ=255)
                                        cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                              >  1:3786913/1‑71 (MQ=255)
                                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:3582098/48‑1 (MQ=255)
                                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             <  1:760596/55‑1 (MQ=255)
                                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             <  1:2906819/55‑1 (MQ=255)
                                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                         >  1:621877/1‑59 (MQ=255)
                                            gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg                           <  1:3169164/70‑1 (MQ=255)
                                              gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                                      <  1:1333000/57‑1 (MQ=255)
                                               tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc                                                        <  1:3442447/38‑1 (MQ=255)
                                               tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                             <  1:624552/49‑1 (MQ=255)
                                               tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc                          <  1:296592/68‑1 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                     >  1:3038211/1‑71 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                     >  1:2927155/1‑71 (MQ=255)
                                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                     >  1:3431161/1‑71 (MQ=255)
                                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                     >  1:376794/1‑68 (MQ=255)
                                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                    <  1:2395589/71‑1 (MQ=255)
                                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                  >  1:2888102/1‑41 (MQ=255)
                                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt                    <  1:1204715/71‑1 (MQ=255)
                                                   ctctctGGTCGTCATCAACTGTATCAGGATCACCAGTGGTTGCGTGATTTCGGTGAAAGCCTGCTGGtt                     >  1:3081832/1‑69 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   >  1:3618344/1‑71 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   >  1:3258132/1‑71 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   >  1:1420501/1‑71 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   <  1:171778/71‑1 (MQ=255)
                                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTCGttt                    >  1:1739815/1‑70 (MQ=255)
                                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                    <  1:1971055/35‑1 (MQ=255)
                                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                               <  1:3556461/56‑1 (MQ=255)
                                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                               <  1:1841674/56‑1 (MQ=255)
                                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt                  <  1:1090916/69‑1 (MQ=255)
                                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt                  <  1:2968038/69‑1 (MQ=255)
                                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt               >  1:1377955/1‑71 (MQ=255)
                                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                 <  1:3732729/68‑1 (MQ=255)
                                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                <  1:1502069/69‑1 (MQ=255)
                                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc              >  1:492068/1‑71 (MQ=255)
                                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc          >  1:4025184/1‑70 (MQ=255)
                                                              tcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                   <  1:58550/60‑1 (MQ=255)
                                                              tcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa        <  1:2264337/71‑1 (MQ=255)
                                                              ccagcagCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                    <  1:3229760/58‑1 (MQ=255)
                                                               cAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc              >  1:841922/1‑64 (MQ=255)
                                                                agcagcTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATc      <  1:2108357/71‑1 (MQ=255)
                                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc      <  1:4009340/71‑1 (MQ=255)
                                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc      <  1:3856566/71‑1 (MQ=255)
                                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc      >  1:3508892/1‑71 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATGGTCCGCCGa        <  1:3037794/68‑1 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg     <  1:3482321/71‑1 (MQ=255)
                                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCAGCCGATc      >  1:2217536/1‑70 (MQ=255)
                                                                  cAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                 >  1:316846/1‑58 (MQ=255)
                                                                    aCTGTATCAGGTTCACCAGTGGATGCGTGATTTCg                                      <  1:3084257/35‑1 (MQ=255)
                                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                  >  1:312066/1‑39 (MQ=255)
                                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca  <  1:464190/71‑1 (MQ=255)
                                                                    |                                                                      
TCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACA  >  minE/834606‑834744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: