Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,691 A→C 9.4% Q1050P (CAG→CCG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,6910AC 9.4% 205.4 / 17.7 85Q1050P (CAG→CCG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (37/40);  new base C (3/5);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAA  >  minE/834622‑834761
                                                                     |                                                                      
acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                                       >  1:670615/1‑71 (MQ=255)
acacCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAg                                                                       >  1:272026/1‑71 (MQ=255)
  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGGAACTGTATCAGGATCACCAGTg                                                                     <  1:1025442/71‑1 (MQ=255)
  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     <  1:3576880/71‑1 (MQ=255)
  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     <  1:3505227/71‑1 (MQ=255)
  acCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     >  1:3068521/1‑71 (MQ=255)
   ccAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     <  1:3910876/70‑1 (MQ=255)
     aaCGTTGACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                                   >  1:538444/1‑70 (MQ=255)
     aaCGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGAt                                                                  >  1:1990523/1‑71 (MQ=255)
         ttCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                                                     <  1:1730817/64‑1 (MQ=255)
          tCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTg                                                                     <  1:3558390/63‑1 (MQ=255)
              gAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCa                                                                        <  1:1271156/56‑1 (MQ=255)
               aGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTc                                                        <  1:2607396/71‑1 (MQ=255)
                    aTCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                                   <  1:2740569/71‑1 (MQ=255)
                     tCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                                   >  1:664217/1‑70 (MQ=255)
                      cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                              >  1:1653097/1‑58 (MQ=255)
                      cccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                              >  1:2933068/1‑58 (MQ=255)
                       ccATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                                 <  1:115876/70‑1 (MQ=255)
                        cATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGc                                               >  1:3786913/1‑71 (MQ=255)
                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     <  1:3582098/48‑1 (MQ=255)
                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                              <  1:760596/55‑1 (MQ=255)
                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                              <  1:2906819/55‑1 (MQ=255)
                         aTGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAtt                                                          >  1:621877/1‑59 (MQ=255)
                            gCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctg                                            <  1:3169164/70‑1 (MQ=255)
                              gTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCg                                                       <  1:1333000/57‑1 (MQ=255)
                               tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGt                                                              <  1:624552/49‑1 (MQ=255)
                               tACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCctgc                                           <  1:296592/68‑1 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                      >  1:3431161/1‑71 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                      >  1:2927155/1‑71 (MQ=255)
                                 cGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGtt                                      >  1:3038211/1‑71 (MQ=255)
                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCtggtggct                                      >  1:376794/1‑68 (MQ=255)
                                  gCTGTCGGGACGCCAGCAGCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                                     <  1:2395589/71‑1 (MQ=255)
                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGa                                                                   >  1:2888102/1‑41 (MQ=255)
                                  gCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGttt                                     <  1:1204715/71‑1 (MQ=255)
                                   ctctctGGTCGTCATCAACTGTATCAGGATCACCAGTGGTTGCGTGATTTCGGTGAAAGCCTGCTGGtt                                      >  1:3081832/1‑69 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                    >  1:3618344/1‑71 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                    >  1:3258132/1‑71 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                    >  1:1420501/1‑71 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                    <  1:171778/71‑1 (MQ=255)
                                   ctctctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTCGttt                                     >  1:1739815/1‑70 (MQ=255)
                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTg                                                                     <  1:1971055/35‑1 (MQ=255)
                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                                <  1:3556461/56‑1 (MQ=255)
                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAg                                                <  1:1841674/56‑1 (MQ=255)
                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt                                   <  1:2968038/69‑1 (MQ=255)
                                      tctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTAt                                   <  1:1090916/69‑1 (MQ=255)
                                       ctGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGt                                >  1:1377955/1‑71 (MQ=255)
                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                                  <  1:3732729/68‑1 (MQ=255)
                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCg                                 <  1:1502069/69‑1 (MQ=255)
                                        tGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                               >  1:492068/1‑71 (MQ=255)
                                             gtcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc                           >  1:4025184/1‑70 (MQ=255)
                                              tcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTa                                    <  1:58550/60‑1 (MQ=255)
                                              tcAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa                         <  1:2264337/71‑1 (MQ=255)
                                              ccagcagCTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGctt                                     <  1:3229760/58‑1 (MQ=255)
                                               cAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTc                               >  1:841922/1‑64 (MQ=255)
                                                agcagcTCTATCAGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATc                       <  1:2108357/71‑1 (MQ=255)
                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                       <  1:4009340/71‑1 (MQ=255)
                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                       >  1:3508892/1‑71 (MQ=255)
                                                aGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                       <  1:3856566/71‑1 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATGGTCCGCCGa                         <  1:3037794/68‑1 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCg                      <  1:3482321/71‑1 (MQ=255)
                                                 gCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCAGCCGATc                       >  1:2217536/1‑70 (MQ=255)
                                                  cAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATc                                  >  1:316846/1‑58 (MQ=255)
                                                    aCTGTATCAGGTTCACCAGTGGATGCGTGATTTCg                                                       <  1:3084257/35‑1 (MQ=255)
                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGa                                                   >  1:312066/1‑39 (MQ=255)
                                                    aCTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGaca                   <  1:464190/71‑1 (MQ=255)
                                                     cTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGacac                  >  1:273610/1‑71 (MQ=255)
                                                        tATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCg               >  1:652261/1‑71 (MQ=255)
                                                          tCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                                 >  1:1109321/1‑35 (MQ=255)
                                                          tCAGGATCACCAGTGGATGCGTGATTTCGGTGaaa                                                 >  1:3048256/1‑35 (MQ=255)
                                                            aGGATCATCCGTGGATGCGTGCTTTTGGTGAAAGCCTGGTGGCTTATCGCCCGCCTATCGACACCCGtagc           >  1:453748/1‑68 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGGCGATc                       >  1:1671089/1‑59 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCg           >  1:3456817/1‑71 (MQ=255)
                                                            aGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCg           >  1:2655381/1‑71 (MQ=255)
                                                              gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACAcc                 >  1:299418/1‑63 (MQ=255)
                                                              gATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTACGCCGATCGACACCCGTTCGGt         >  1:2383942/1‑71 (MQ=255)
                                                               aTCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGa                         <  1:3650481/54‑1 (MQ=255)
                                                               aTCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATc                       <  1:1905640/56‑1 (MQ=255)
                                                                tCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGa       <  1:2022762/71‑1 (MQ=255)
                                                                 cACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGa                     >  1:2732159/1‑56 (MQ=255)
                                                                  aCCAGTGGATGCGTGATTTCGGTGAAAGCCTTCTGGTTTa                                    <  1:2231752/40‑1 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    <  1:2152615/71‑1 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    <  1:2265906/71‑1 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    <  1:2330414/71‑1 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    <  1:1223672/71‑1 (MQ=255)
                                                                   ccAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    <  1:250474/71‑1 (MQ=255)
                                                                    cAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    >  1:213404/1‑70 (MQ=255)
                                                                     aGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaagaa  <  1:16699/71‑1 (MQ=255)
                                                                     aGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAaag    >  1:237556/1‑69 (MQ=255)
                                                                     |                                                                      
ACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAA  >  minE/834622‑834761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: