| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 51,837 | G→A | 50.0% | P182S (CCG→TCG) | surA ← | peptidyl‑prolyl cis‑trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 51,837 | 0 | G | A | 50.0% | ‑4.7 / 54.4 | 38 | P182S (CCG→TCG) | surA | peptidyl‑prolyl cis‑trans isomerase |
| Reads supporting (aligned to +/- strand): ref base G (9/10); new base A (12/7); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01 | |||||||||||
AATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGC > minE/51775‑51900 | aaTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGa < 1:377081/71‑1 (MQ=255) tGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGAt > 1:3536380/1‑64 (MQ=255) tGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGa < 1:3884953/69‑1 (MQ=255) ggCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGAtg > 1:191272/1‑70 (MQ=255) ggCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGAGATCAGGAtgt > 1:302153/1‑71 (MQ=255) tGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGtt > 1:1868770/1‑71 (MQ=255) ggCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGtt > 1:2621379/1‑70 (MQ=255) gCTTTCCGCTTCGTTCACCTGAGCAGAGGTCGGGTTTTCCGGCAGCGAGATCAGGa < 1:3639870/56‑1 (MQ=255) tCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCt < 1:4046938/70‑1 (MQ=255) gCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTc > 1:4083605/1‑68 (MQ=255) gCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAg > 1:3325109/1‑70 (MQ=255) gCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGAGATCAg < 1:100089/47‑1 (MQ=255) cGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTg > 1:2714662/1‑71 (MQ=255) gTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTc < 1:211713/52‑1 (MQ=255) cACCTGATCAGAGGTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgt > 1:2792661/1‑71 (MQ=255) tGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtt > 1:2732729/1‑71 (MQ=255) cAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTgg > 1:3664184/1‑71 (MQ=255) agagGTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCa < 1:1317068/47‑1 (MQ=255) agagGTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGt > 1:530868/1‑71 (MQ=255) agGTCGGGTTTTACGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGc > 1:2500684/1‑47 (MQ=255) ggTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTAc < 1:182681/71‑1 (MQ=255) ggTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAg > 1:2337330/1‑39 (MQ=255) gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt < 1:1769662/64‑1 (MQ=255) gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt < 1:3784662/64‑1 (MQ=255) gTCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGcgtcg < 1:2826156/60‑1 (MQ=255) tCGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCa < 1:1885946/42‑1 (MQ=255) cGGGTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCa > 1:3629740/1‑71 (MQ=255) gggTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc > 1:1619224/1‑71 (MQ=255) gggTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCAc > 1:2528306/1‑71 (MQ=255) gTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCt < 1:2047272/41‑1 (MQ=255) gTTTTCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACct < 1:1327769/71‑1 (MQ=255) ttttCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt > 1:2757001/1‑58 (MQ=255) ttttCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGttt > 1:2997923/1‑57 (MQ=255) ttCCGGCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACctgct > 1:447974/1‑71 (MQ=255) ggCATCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcgc < 1:2513238/71‑1 (MQ=255) gCAGCGAGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTgcgc > 1:2515398/1‑70 (MQ=255) gCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt < 1:323768/48‑1 (MQ=255) gCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGtttt < 1:1228423/48‑1 (MQ=255) | AATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGC > minE/51775‑51900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |