Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,918 A→C 6.6% K1126Q (AAA→CAA) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9180AC 6.6% 156.7 / 6.6 61K1126Q (AAA→CAA) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (19/38);  new base C (2/2);  total (21/40)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

CAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTG  >  minE/834848‑834987
                                                                      |                                                                     
cAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCa                                                                       <  1:2774346/71‑1 (MQ=255)
 aGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCaa                                                                      <  1:3696262/71‑1 (MQ=255)
   cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:4030723/71‑1 (MQ=255)
   cGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:2487281/71‑1 (MQ=255)
    gACAACCTGCTGATGCTGACTTTAGGTCGCGGGGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:604000/70‑1 (MQ=255)
       aaCCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCt                                                                >  1:55885/1‑71 (MQ=255)
         ccTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTgg                                                              >  1:939944/1‑71 (MQ=255)
             ctgatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGt                                                            <  1:2698323/69‑1 (MQ=255)
               gatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCg                                                        >  1:3245974/1‑71 (MQ=255)
                atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                         <  1:2356704/69‑1 (MQ=255)
                atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                         <  1:660302/69‑1 (MQ=255)
                 tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                      >  1:1517154/1‑71 (MQ=255)
                 tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                      <  1:1935477/71‑1 (MQ=255)
                 tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                      >  1:94788/1‑71 (MQ=255)
                 tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                      >  1:3241079/1‑71 (MQ=255)
                 tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                       >  1:3506100/1‑70 (MQ=255)
                  gctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                      >  1:3800049/1‑70 (MQ=255)
                   ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                       >  1:1744017/1‑68 (MQ=255)
                   ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                    >  1:3017471/1‑71 (MQ=255)
                   ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                    >  1:101767/1‑71 (MQ=255)
                    tgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:532098/54‑1 (MQ=255)
                    tgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:332618/54‑1 (MQ=255)
                      aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   <  1:2235862/69‑1 (MQ=255)
                      aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   <  1:2692664/69‑1 (MQ=255)
                      aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   <  1:1084031/69‑1 (MQ=255)
                      aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATaa                                                 <  1:2160717/71‑1 (MQ=255)
                       cTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAAc                                                <  1:792321/71‑1 (MQ=255)
                              tCGCGGTGGACCGATTGTCTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTggg                                         >  1:4009463/1‑70 (MQ=255)
                               cgcgGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTg                                                               >  1:1646543/1‑48 (MQ=255)
                                 cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                      <  1:3708212/71‑1 (MQ=255)
                                 cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                      <  1:1986199/71‑1 (MQ=255)
                                   gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAg                                                                    <  1:2596766/39‑1 (MQ=255)
                                   gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   <  1:719589/56‑1 (MQ=255)
                                   gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   <  1:1035404/56‑1 (MQ=255)
                                    tggtccggtggtcTGGTTGAGTGAAGCCGATGCCa                                                                       <  1:2245918/35‑1 (MQ=255)
                                        ccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                    >  1:652050/1‑66 (MQ=255)
                                             tgtcTGGATCAGGGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcg                          >  1:1531574/2‑70 (MQ=255)
                                              gtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAg                         <  1:2990809/71‑1 (MQ=255)
                                               tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                        <  1:1218425/71‑4 (MQ=255)
                                               tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                        <  1:1700512/71‑4 (MQ=255)
                                                 tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                         <  1:2242557/36‑1 (MQ=255)
                                                 tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACg                                               <  1:2513791/46‑1 (MQ=255)
                                                 tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTgg                                          <  1:240110/51‑1 (MQ=255)
                                                 tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                      <  1:3865997/55‑1 (MQ=255)
                                                   gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                       <  1:3940459/36‑1 (MQ=255)
                                                   gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                    >  1:3610945/1‑55 (MQ=255)
                                                   gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCATAACGACTGGATTGAAGTCTTCAACAGCAAc                     <  1:1318900/69‑1 (MQ=255)
                                                    ttGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCg                                                     <  1:701745/37‑1 (MQ=255)
                                                     tGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                  >  1:3302810/1‑71 (MQ=255)
                                                      gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                   >  1:3626790/1‑37 (MQ=255)
                                                      gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                  <  1:993559/70‑1 (MQ=255)
                                                        gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGc                >  1:2477386/1‑70 (MQ=255)
                                                         tGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCaa                            <  1:594398/57‑1 (MQ=255)
                                                           acgCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct             <  1:3687096/68‑1 (MQ=255)
                                                           aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGc                        <  1:2599645/59‑1 (MQ=255)
                                                           aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct             <  1:2021943/70‑1 (MQ=255)
                                                            aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGa                                              <  1:2385670/36‑1 (MQ=255)
                                                             gCCGATGCCAAAGATCTGGGTATCGCCGATAACGAc                                             >  1:1357909/1‑36 (MQ=255)
                                                             gCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct               <  1:1768966/66‑1 (MQ=255)
                                                                 aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct             <  1:4003014/64‑1 (MQ=255)
                                                                 aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct             <  1:3229268/64‑1 (MQ=255)
                                                                 aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGcc      <  1:414273/71‑1 (MQ=255)
                                                                   gCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg    >  1:3341898/1‑71 (MQ=255)
                                                                     cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTg  <  1:4058024/71‑1 (MQ=255)
                                                                      aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGAc          >  1:1402149/1‑62 (MQ=255)
                                                                      |                                                                     
CAGCGACAACCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTG  >  minE/834848‑834987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: