Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,927 G→A 7.0% G1129S (GGT→AGT) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9270GA 7.0% 181.2 / 2.4 57G1129S (GGT→AGT) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (20/33);  new base A (2/2);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAG  >  minE/834857‑834997
                                                                      |                                                                      
ccTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTgg                                                                        >  1:939944/1‑71 (MQ=255)
    ctgatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGt                                                                      <  1:2698323/69‑1 (MQ=255)
      gatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCg                                                                  >  1:3245974/1‑71 (MQ=255)
       atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                   <  1:2356704/69‑1 (MQ=255)
       atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                   <  1:660302/69‑1 (MQ=255)
        tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                >  1:3241079/1‑71 (MQ=255)
        tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                <  1:1935477/71‑1 (MQ=255)
        tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                >  1:1517154/1‑71 (MQ=255)
        tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                >  1:94788/1‑71 (MQ=255)
        tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                 >  1:3506100/1‑70 (MQ=255)
         gctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                >  1:3800049/1‑70 (MQ=255)
          ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                 >  1:1744017/1‑68 (MQ=255)
          ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                              >  1:3017471/1‑71 (MQ=255)
          ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                              >  1:101767/1‑71 (MQ=255)
             aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             <  1:2235862/69‑1 (MQ=255)
             aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             <  1:2692664/69‑1 (MQ=255)
             aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             <  1:1084031/69‑1 (MQ=255)
             aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATaa                                                           <  1:2160717/71‑1 (MQ=255)
              cTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAAc                                                          <  1:792321/71‑1 (MQ=255)
                     tCGCGGTGGACCGATTGTCTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTggg                                                   >  1:4009463/1‑70 (MQ=255)
                        cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                <  1:3708212/71‑1 (MQ=255)
                        cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                <  1:1986199/71‑1 (MQ=255)
                          gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             <  1:1035404/56‑1 (MQ=255)
                          gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             <  1:719589/56‑1 (MQ=255)
                               ccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                              >  1:652050/1‑66 (MQ=255)
                                    tgtcTGGATCAGGGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcg                                    >  1:1531574/2‑70 (MQ=255)
                                     gtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAg                                   <  1:2990809/71‑1 (MQ=255)
                                      tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                  <  1:1700512/71‑4 (MQ=255)
                                      tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                  <  1:1218425/71‑4 (MQ=255)
                                        tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                   <  1:2242557/36‑1 (MQ=255)
                                        tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACg                                                         <  1:2513791/46‑1 (MQ=255)
                                        tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTgg                                                    <  1:240110/51‑1 (MQ=255)
                                        tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                <  1:3865997/55‑1 (MQ=255)
                                          gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                 <  1:3940459/36‑1 (MQ=255)
                                          gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                              >  1:3610945/1‑55 (MQ=255)
                                          gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCATAACGACTGGATTGAAGTCTTCAACAGCAAc                               <  1:1318900/69‑1 (MQ=255)
                                           ttGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCg                                                               <  1:701745/37‑1 (MQ=255)
                                            tGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                            >  1:3302810/1‑71 (MQ=255)
                                             gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                             >  1:3626790/1‑37 (MQ=255)
                                             gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                            <  1:993559/70‑1 (MQ=255)
                                               gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGc                          >  1:2477386/1‑70 (MQ=255)
                                                tGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCaa                                      <  1:594398/57‑1 (MQ=255)
                                                  acgCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                       <  1:3687096/68‑1 (MQ=255)
                                                  aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGc                                  <  1:2599645/59‑1 (MQ=255)
                                                  aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                       <  1:2021943/70‑1 (MQ=255)
                                                   aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGa                                                        <  1:2385670/36‑1 (MQ=255)
                                                    gCCGATGCCAAAGATCTGGGTATCGCCGATAACGAc                                                       >  1:1357909/1‑36 (MQ=255)
                                                    gCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                         <  1:1768966/66‑1 (MQ=255)
                                                        aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                       <  1:4003014/64‑1 (MQ=255)
                                                        aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                       <  1:3229268/64‑1 (MQ=255)
                                                        aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGcc                <  1:414273/71‑1 (MQ=255)
                                                          gCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg              >  1:3341898/1‑71 (MQ=255)
                                                            cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTg            <  1:4058024/71‑1 (MQ=255)
                                                             aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGAc                    >  1:1402149/1‑62 (MQ=255)
                                                              aaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCg          <  1:1399246/71‑1 (MQ=255)
                                                               aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg         >  1:2740558/1‑71 (MQ=255)
                                                               aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg         >  1:1542023/1‑71 (MQ=255)
                                                                   cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt     >  1:1915694/1‑71 (MQ=255)
                                                                      ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcag  >  1:3381045/1‑71 (MQ=255)
                                                                      |                                                                      
CCTGCTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAG  >  minE/834857‑834997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: