Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,928 G→C 7.0% G1129A (GGT→GCT) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9280GC 7.0% 175.2 / 3.2 57G1129A (GGT→GCT) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (20/33);  new base C (2/2);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

CTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGC  >  minE/834861‑834998
                                                                   |                                                                      
ctgatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGt                                                                       <  1:2698323/69‑1 (MQ=255)
  gatgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCg                                                                   >  1:3245974/1‑71 (MQ=255)
   atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                    <  1:2356704/69‑1 (MQ=255)
   atgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                    <  1:660302/69‑1 (MQ=255)
    tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:94788/1‑71 (MQ=255)
    tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 <  1:1935477/71‑1 (MQ=255)
    tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:3241079/1‑71 (MQ=255)
    tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:1517154/1‑71 (MQ=255)
    tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  >  1:3506100/1‑70 (MQ=255)
     gctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:3800049/1‑70 (MQ=255)
      ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  >  1:1744017/1‑68 (MQ=255)
      ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                               >  1:3017471/1‑71 (MQ=255)
      ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                               >  1:101767/1‑71 (MQ=255)
         aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:1084031/69‑1 (MQ=255)
         aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:2235862/69‑1 (MQ=255)
         aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:2692664/69‑1 (MQ=255)
         aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATaa                                                            <  1:2160717/71‑1 (MQ=255)
          cTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAAc                                                           <  1:792321/71‑1 (MQ=255)
                 tCGCGGTGGACCGATTGTCTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTggg                                                    >  1:4009463/1‑70 (MQ=255)
                    cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:3708212/71‑1 (MQ=255)
                    cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:1986199/71‑1 (MQ=255)
                      gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:1035404/56‑1 (MQ=255)
                      gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:719589/56‑1 (MQ=255)
                           ccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                               >  1:652050/1‑66 (MQ=255)
                                tgtcTGGATCAGGGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcg                                     >  1:1531574/2‑70 (MQ=255)
                                 gtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAg                                    <  1:2990809/71‑1 (MQ=255)
                                  tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                   <  1:1218425/71‑4 (MQ=255)
                                  tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                   <  1:1700512/71‑4 (MQ=255)
                                    tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATc                                                                    <  1:2242557/36‑1 (MQ=255)
                                    tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACg                                                          <  1:2513791/46‑1 (MQ=255)
                                    tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTgg                                                     <  1:240110/51‑1 (MQ=255)
                                    tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:3865997/55‑1 (MQ=255)
                                      gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  <  1:3940459/36‑1 (MQ=255)
                                      gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                               >  1:3610945/1‑55 (MQ=255)
                                      gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCATAACGACTGGATTGAAGTCTTCAACAGCAAc                                <  1:1318900/69‑1 (MQ=255)
                                       ttGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCg                                                                <  1:701745/37‑1 (MQ=255)
                                        tGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                             >  1:3302810/1‑71 (MQ=255)
                                         gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              >  1:3626790/1‑37 (MQ=255)
                                         gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                             <  1:993559/70‑1 (MQ=255)
                                           gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGc                           >  1:2477386/1‑70 (MQ=255)
                                            tGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCaa                                       <  1:594398/57‑1 (MQ=255)
                                              acgCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:3687096/68‑1 (MQ=255)
                                              aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGc                                   <  1:2599645/59‑1 (MQ=255)
                                              aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:2021943/70‑1 (MQ=255)
                                               aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGa                                                         <  1:2385670/36‑1 (MQ=255)
                                                gCCGATGCCAAAGATCTGGGTATCGCCGATAACGAc                                                        >  1:1357909/1‑36 (MQ=255)
                                                gCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                          <  1:1768966/66‑1 (MQ=255)
                                                    aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:3229268/64‑1 (MQ=255)
                                                    aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:4003014/64‑1 (MQ=255)
                                                    aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGcc                 <  1:414273/71‑1 (MQ=255)
                                                      gCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg               >  1:3341898/1‑71 (MQ=255)
                                                        cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTg             <  1:4058024/71‑1 (MQ=255)
                                                         aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGAc                     >  1:1402149/1‑62 (MQ=255)
                                                          aaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCg           <  1:1399246/71‑1 (MQ=255)
                                                           aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg          >  1:2740558/1‑71 (MQ=255)
                                                           aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg          >  1:1542023/1‑71 (MQ=255)
                                                               cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt      >  1:1915694/1‑71 (MQ=255)
                                                                  ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcag   >  1:3381045/1‑71 (MQ=255)
                                                                   gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc  <  1:1522694/71‑1 (MQ=255)
                                                                   |                                                                      
CTGATGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGC  >  minE/834861‑834998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: