Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 834,934 C→T 7.4% A1131V (GCC→GTC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE834,9340CT 7.4% 169.2 / 3.4 54A1131V (GCC→GTC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (16/34);  new base T (2/2);  total (18/36)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGC  >  minE/834865‑834998
                                                                     |                                                                
tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 <  1:1935477/71‑1 (MQ=255)
tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:3241079/1‑71 (MQ=255)
tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:1517154/1‑71 (MQ=255)
tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:94788/1‑71 (MQ=255)
tgctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  >  1:3506100/1‑70 (MQ=255)
 gctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGcc                                                                 >  1:3800049/1‑70 (MQ=255)
  ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  >  1:1744017/1‑68 (MQ=255)
  ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                               >  1:101767/1‑71 (MQ=255)
  ctgaCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGa                                                               >  1:3017471/1‑71 (MQ=255)
     aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:2692664/69‑1 (MQ=255)
     aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:1084031/69‑1 (MQ=255)
     aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:2235862/69‑1 (MQ=255)
     aCTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATaa                                                            <  1:2160717/71‑1 (MQ=255)
      cTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAAc                                                           <  1:792321/71‑1 (MQ=255)
             tCGCGGTGGACCGATTGTCTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTggg                                                    >  1:4009463/1‑70 (MQ=255)
                cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:3708212/71‑1 (MQ=255)
                cggtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:1986199/71‑1 (MQ=255)
                  gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:1035404/56‑1 (MQ=255)
                  gtggtccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              <  1:719589/56‑1 (MQ=255)
                       ccggtggtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                               >  1:652050/1‑66 (MQ=255)
                            tgtcTGGATCAGGGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcg                                     >  1:1531574/2‑70 (MQ=255)
                             gtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAg                                    <  1:2990809/71‑1 (MQ=255)
                              tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                   <  1:1700512/71‑4 (MQ=255)
                              tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgcc                                   <  1:1218425/71‑4 (MQ=255)
                                tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACg                                                          <  1:2513791/46‑1 (MQ=255)
                                tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTgg                                                     <  1:240110/51‑1 (MQ=255)
                                tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTg                                                 <  1:3865997/55‑1 (MQ=255)
                                  gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGc                                                                  <  1:3940459/36‑1 (MQ=255)
                                  gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGaa                                               >  1:3610945/1‑55 (MQ=255)
                                  gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCATAACGACTGGATTGAAGTCTTCAACAGCAAc                                <  1:1318900/69‑1 (MQ=255)
                                   ttGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCg                                                                <  1:701745/37‑1 (MQ=255)
                                    tGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                             >  1:3302810/1‑71 (MQ=255)
                                     gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGAt                                                              >  1:3626790/1‑37 (MQ=255)
                                     gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGt                             <  1:993559/70‑1 (MQ=255)
                                       gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGc                           >  1:2477386/1‑70 (MQ=255)
                                        tGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCaa                                       <  1:594398/57‑1 (MQ=255)
                                          acgCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:3687096/68‑1 (MQ=255)
                                          aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGc                                   <  1:2599645/59‑1 (MQ=255)
                                          aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:2021943/70‑1 (MQ=255)
                                           aGCCGATGCCAAAGATCTGGGTATCGCCGATAACGa                                                         <  1:2385670/36‑1 (MQ=255)
                                            gCCGATGCCAAAGATCTGGGTATCGCCGATAACGAc                                                        >  1:1357909/1‑36 (MQ=255)
                                            gCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                          <  1:1768966/66‑1 (MQ=255)
                                                aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:3229268/64‑1 (MQ=255)
                                                aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:4003014/64‑1 (MQ=255)
                                                aTGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGcc                 <  1:414273/71‑1 (MQ=255)
                                                  gCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCg               >  1:3341898/1‑71 (MQ=255)
                                                    cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTg             <  1:4058024/71‑1 (MQ=255)
                                                     aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGAc                     >  1:1402149/1‑62 (MQ=255)
                                                      aaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCg           <  1:1399246/71‑1 (MQ=255)
                                                       aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg          >  1:2740558/1‑71 (MQ=255)
                                                       aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg          >  1:1542023/1‑71 (MQ=255)
                                                           cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt      >  1:1915694/1‑71 (MQ=255)
                                                              ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcag   >  1:3381045/1‑71 (MQ=255)
                                                               gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc  <  1:1522694/71‑1 (MQ=255)
                                                                tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTg             <  1:570995/59‑1 (MQ=255)
                                                                tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc  <  1:1667017/70‑1 (MQ=255)
                                                                 aTCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc  >  1:3090797/1‑69 (MQ=255)
                                                                  tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:1584379/46‑1 (MQ=255)
                                                                  tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:2454352/46‑1 (MQ=255)
                                                                  tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                        <  1:3158/46‑1 (MQ=255)
                                                                   cGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTg                       <  1:1534478/46‑1 (MQ=255)
                                                                    gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACt                    >  1:369652/1‑48 (MQ=255)
                                                                     |                                                                
TGCTGACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGC  >  minE/834865‑834998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: