| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,190 | T→A | 7.1% | G1216G (GGT→GGA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,190 | 0 | T | A | 7.1% | 278.7 / 11.5 | 99 | G1216G (GGT→GGA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (46/46); new base A (4/3); total (50/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
AACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGG > minE/835123‑835255 | aaCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:1006126/71‑1 (MQ=255) ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa < 1:3191659/71‑1 (MQ=255) cGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAc < 1:2074633/71‑1 (MQ=255) cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc < 1:357503/71‑1 (MQ=255) cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc < 1:199442/71‑1 (MQ=255) gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg < 1:355622/71‑1 (MQ=255) tatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACcgc < 1:2481663/67‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:1229878/61‑1 (MQ=255) tatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACGACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3786174/1‑70 (MQ=255) tGATTGGAGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAg > 1:640141/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGtt > 1:436609/1‑57 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa > 1:3481785/1‑61 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg < 1:3793958/71‑1 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:3108269/1‑71 (MQ=255) tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg > 1:1762871/1‑71 (MQ=255) aTCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGt < 1:691408/70‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGTTTCTAACCGCGATGAGTttgttg < 1:245151/71‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:2732991/71‑1 (MQ=255) cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGGttgttg < 1:6775/71‑1 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:3279475/1‑70 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:671076/1‑70 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:1233875/1‑68 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3688752/71‑1 (MQ=255) tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:1902457/1‑71 (MQ=255) tATGCCCACCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:3935296/1‑71 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAGCGCGATGAGTTTGTTGTAGTg > 1:71732/1‑71 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg > 1:3152808/1‑71 (MQ=255) tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt < 1:2876800/46‑1 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTa < 1:230824/71‑1 (MQ=255) cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAg < 1:1089940/54‑1 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAc > 1:3170432/1‑44 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAc > 1:2290763/1‑44 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:1865410/1‑68 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:1606182/56‑1 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:2799776/56‑1 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCg > 1:3939148/1‑66 (MQ=255) cTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGa > 1:1912363/1‑70 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1387017/57‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2129653/58‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:1560120/1‑59 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:2168586/1‑59 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTaa < 1:1844840/67‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt > 1:192605/1‑70 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGAAGGTTCTAACCGCGATGa > 1:3531384/1‑49 (MQ=255) ggCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3085191/56‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGttt > 1:2605526/1‑51 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt < 1:2851865/68‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa < 1:604381/71‑1 (MQ=255) cATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag < 1:4072356/71‑1 (MQ=255) aTACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaaga < 1:182280/71‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGa > 1:3629713/1‑41 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:2334095/52‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1714691/52‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1260439/52‑1 (MQ=255) gggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAc < 1:2679682/69‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg > 1:3656587/1‑41 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGa > 1:558235/1‑42 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:601140/1‑69 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3651660/1‑69 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:2453992/70‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:3309420/70‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:2788969/1‑71 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:1816317/71‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:1743056/1‑71 (MQ=255) ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa < 1:232103/68‑1 (MQ=255) gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg > 1:1984950/1‑45 (MQ=255) gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:674326/71‑1 (MQ=255) gCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtt < 1:1072415/71‑1 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:2875070/1‑68 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:1492233/71‑1 (MQ=255) tttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2800791/47‑1 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:157987/1‑41 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:3011530/1‑41 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:584510/1‑45 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:1524225/1‑71 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:1446072/1‑71 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:3964302/1‑71 (MQ=255) actactATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTgg > 1:3977686/1‑71 (MQ=255) ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:1107993/42‑1 (MQ=255) actaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:2601759/1‑69 (MQ=255) actaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg > 1:2101860/1‑59 (MQ=255) actaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg > 1:761130/1‑59 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:3940512/1‑36 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:3308921/1‑68 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:436350/1‑68 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3464078/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1400548/70‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATga < 1:3062234/50‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc > 1:3293592/1‑55 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa > 1:1825414/2‑54 (MQ=255) gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3418545/1‑52 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:3299478/71‑1 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:834316/71‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:70176/51‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg < 1:2883082/66‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAG‑TTGTTGTAGTGCGTAAGATGAAGAACa < 1:3281758/50‑1 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTATTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg > 1:4022729/1‑70 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:2933549/1‑54 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg > 1:233002/1‑70 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:2801162/64‑1 (MQ=255) | AACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGG > minE/835123‑835255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |