| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,209 | G→A | 5.7% | V1223I (GTT→ATT) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,209 | 0 | G | A | 5.7% | 291.7 / 5.0 | 88 | V1223I (GTT→ATT) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (44/39); new base A (3/2); total (47/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG > minE/835142‑835278 | cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGTTTCTAACCGCGATGAGTttgttg < 1:245151/71‑1 (MQ=255)cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:2732991/71‑1 (MQ=255)cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGGttgttg < 1:6775/71‑1 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:3279475/1‑70 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:1233875/1‑68 (MQ=255) ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3688752/71‑1 (MQ=255) tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:1902457/1‑71 (MQ=255) tATGCCCACCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:3935296/1‑71 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAAGCGCGATGAGTTTGTTGTAGTg > 1:71732/1‑71 (MQ=255) aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg > 1:3152808/1‑71 (MQ=255) ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTa < 1:230824/71‑1 (MQ=255) aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:1865410/1‑68 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:2799776/56‑1 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg < 1:1606182/56‑1 (MQ=255) tCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCg > 1:3939148/1‑66 (MQ=255) cTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGa > 1:1912363/1‑70 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1387017/57‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2129653/58‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:1560120/1‑59 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:2168586/1‑59 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTaa < 1:1844840/67‑1 (MQ=255) tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt > 1:192605/1‑70 (MQ=255) ggCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3085191/56‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt < 1:2851865/68‑1 (MQ=255) gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa < 1:604381/71‑1 (MQ=255) cATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag < 1:4072356/71‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1714691/52‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:2334095/52‑1 (MQ=255) tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:1260439/52‑1 (MQ=255) gggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAc < 1:2679682/69‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:601140/1‑69 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3651660/1‑69 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:2453992/70‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:3309420/70‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:2788969/1‑71 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:1816317/71‑1 (MQ=255) cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa > 1:1743056/1‑71 (MQ=255) ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa < 1:232103/68‑1 (MQ=255) gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttg > 1:1984950/1‑45 (MQ=255) gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:674326/71‑1 (MQ=255) gCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtt < 1:1072415/71‑1 (MQ=255) cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTg < 1:1492233/71‑1 (MQ=255) tttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2800791/47‑1 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:157987/1‑41 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:3011530/1‑41 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTa > 1:584510/1‑45 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:1524225/1‑71 (MQ=255) aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg > 1:3964302/1‑71 (MQ=255) ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:1107993/42‑1 (MQ=255) actaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg > 1:761130/1‑59 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:3940512/1‑36 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:436350/1‑68 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:3308921/1‑68 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3464078/70‑1 (MQ=255) ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1400548/70‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATga < 1:3062234/50‑1 (MQ=255) tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc > 1:3293592/1‑55 (MQ=255) cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa > 1:1825414/2‑54 (MQ=255) gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3418545/1‑52 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:834316/71‑1 (MQ=255) cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:3299478/71‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:70176/51‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg < 1:2883082/66‑1 (MQ=255) ccGTAGGTTCTAACCGCGATGAG‑TTGTTGTAGTGCGTAAGATGAAGAACa < 1:3281758/50‑1 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTATTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg > 1:4022729/1‑70 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:2933549/1‑54 (MQ=255) tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg > 1:233002/1‑70 (MQ=255) gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:2801162/64‑1 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa > 1:3131654/1‑71 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa > 1:2495360/1‑71 (MQ=255) tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa > 1:2742322/1‑71 (MQ=255) cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa < 1:4099664/70‑1 (MQ=255) cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:1286771/1‑71 (MQ=255) cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:3929115/1‑71 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:2065101/43‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt < 1:2998402/47‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc < 1:1360076/64‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc < 1:1604771/64‑1 (MQ=255) tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATg < 1:3862420/68‑1 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:3994764/1‑69 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:1926929/1‑69 (MQ=255) aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc > 1:1319084/1‑69 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:2785046/70‑1 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa > 1:716929/1‑70 (MQ=255) aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa < 1:2917359/70‑1 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAgg < 1:2236259/71‑1 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAgg < 1:756013/71‑1 (MQ=255) ccGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAgg < 1:1514824/71‑1 (MQ=255) cgcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:3237832/55‑1 (MQ=255) gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:1387209/50‑1 (MQ=255) gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa > 1:2899431/1‑56 (MQ=255) gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa > 1:3286444/1‑67 (MQ=255) cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa > 1:3874902/1‑70 (MQ=255) aTGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAgg < 1:792997/66‑1 (MQ=255) gAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt > 1:4036455/1‑57 (MQ=255) aGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga < 1:265130/70‑1 (MQ=255) tttGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:3866701/39‑1 (MQ=255) tttGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAg < 1:529778/40‑1 (MQ=255) tttGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGagag < 1:1979568/71‑1 (MQ=255) tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg > 1:888426/1‑71 (MQ=255) | CGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG > minE/835142‑835278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |