| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,891 | G→A | 7.6% | K203K (AAG→AAA) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,891 | 0 | G | A | 7.6% | 292.7 / 10.1 | 92 | K203K (AAG→AAA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (41/44); new base A (3/4); total (44/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGG > minE/835821‑835959 | tGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAg > 1:1829469/1‑71 (MQ=255)tGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAg > 1:4045314/1‑71 (MQ=255) gcgcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACgcg < 1:2385307/71‑3 (MQ=255) gcgcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACgcg < 1:373081/71‑3 (MQ=255) cgcCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg > 1:1196786/1‑70 (MQ=255) ccTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag < 1:2951842/71‑1 (MQ=255) tgtgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:1926426/1‑69 (MQ=255) tgtgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:3680443/1‑69 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:669598/69‑1 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:2590296/69‑1 (MQ=255) tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:2782525/70‑1 (MQ=255) cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATg > 1:1010523/1‑71 (MQ=255) cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATg > 1:3143601/1‑71 (MQ=255) aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:1651483/1‑70 (MQ=255) aaCACTGCCTCAA‑TCCCAGATGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACgg > 1:2350301/1‑70 (MQ=255) acacTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa > 1:2775066/1‑64 (MQ=255) acacTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaa > 1:3369353/1‑61 (MQ=255) acacTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCa < 1:71421/71‑1 (MQ=255) acTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag < 1:3717996/60‑1 (MQ=255) tGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:294023/60‑1 (MQ=255) tGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGt > 1:3699780/1‑71 (MQ=255) tGCCTGAA‑‑CCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:39978/59‑1 (MQ=255) ccTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:4021566/1‑71 (MQ=255) ccTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:911336/1‑71 (MQ=255) cTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:1308139/71‑1 (MQ=255) cTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:2695292/71‑1 (MQ=255) aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt > 1:757825/1‑68 (MQ=255) a‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:1386404/1‑55 (MQ=255) a‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:3624603/1‑71 (MQ=255) a‑CCCGGCATGTGTGGCGACATGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:3363334/1‑55 (MQ=255) cccGGCATGTGTGGCGACCTGCGCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:4018316/1‑70 (MQ=255) cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:3070801/1‑70 (MQ=255) ccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:3014250/51‑1 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:4018509/71‑1 (MQ=255) ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:3533152/1‑51 (MQ=255) ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:1090911/1‑51 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:3252888/45‑1 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:1350903/45‑1 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGc < 1:1662162/51‑1 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg < 1:2435676/60‑1 (MQ=255) tgtgtgGCGACATGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGa < 1:1152876/71‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:35998/58‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:1035948/58‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:3808084/58‑1 (MQ=255) tgtgGCGACCTGCCCGAGCGGTTCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:3843982/71‑1 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa > 1:1343375/1‑43 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt < 1:3039922/70‑1 (MQ=255) cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa < 1:201995/68‑1 (MQ=255) ggCGCCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcgt > 1:2612787/1‑52 (MQ=255) gCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt < 1:1605920/70‑1 (MQ=255) gACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:2066572/1‑39 (MQ=255) gACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:2757191/56‑1 (MQ=255) aCCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:2988911/36‑1 (MQ=255) ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:1070637/1‑55 (MQ=255) ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc > 1:2224999/1‑56 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:110676/1‑36 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:2541091/1‑36 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:1070854/1‑54 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg > 1:2985414/1‑71 (MQ=255) ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg < 1:1017280/71‑1 (MQ=255) cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:853566/1‑71 (MQ=255) gAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg < 1:325348/43‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:1439225/48‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:2819306/48‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:2295813/1‑57 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:697619/1‑57 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg > 1:727784/1‑68 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg > 1:1429452/1‑71 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3693529/71‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg > 1:1832239/1‑71 (MQ=255) cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1031246/71‑1 (MQ=255) cGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt < 1:3105730/71‑2 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:2986992/50‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3485686/66‑1 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3744958/1‑71 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt > 1:3754100/1‑37 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt > 1:578630/1‑37 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg < 1:3394863/70‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATGGACCAGGATAAATGCCGt < 1:4032851/53‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg < 1:2983292/54‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:1977388/71‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:4123587/1‑35 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:1857657/1‑71 (MQ=255) tACAAGAGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:539242/1‑70 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc > 1:2352836/1‑54 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc > 1:1667466/1‑54 (MQ=255) aCAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2751332/1‑71 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1445361/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2122629/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2085022/71‑1 (MQ=255) cAAGCGCGAGGAGGACGGCATCGTCCTGATCGACCAGGACAAGTGCCGCGGCTGGCGGATGTGCa < 1:1434330/65‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:1203018/40‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:770933/71‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:3258100/71‑1 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:3903465/1‑40 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:3891022/1‑40 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3126887/54‑1 (MQ=255) | TGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGG > minE/835821‑835959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |