| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,909 | C→T | 5.1% | I209I (ATC→ATT) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,909 | 0 | C | T | 5.1% | 262.3 / 2.7 | 78 | I209I (ATC→ATT) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base C (35/39); new base T (2/2); total (37/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
TGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATC > minE/835841‑835978 | tGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGt > 1:3699780/1‑71 (MQ=255) ccTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:4021566/1‑71 (MQ=255) ccTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:911336/1‑71 (MQ=255) cTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:2695292/71‑1 (MQ=255) cTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:1308139/71‑1 (MQ=255) aaCCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt > 1:757825/1‑68 (MQ=255) aCCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:3624603/1‑71 (MQ=255) cccGGCATGTGTGGCGACCTGCGCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:4018316/1‑70 (MQ=255) cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATc > 1:3070801/1‑70 (MQ=255) cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:4018509/71‑1 (MQ=255) tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg < 1:2435676/60‑1 (MQ=255) tgtgtgGCGACATGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGa < 1:1152876/71‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:35998/58‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:1035948/58‑1 (MQ=255) gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt < 1:3808084/58‑1 (MQ=255) tgtgGCGACCTGCCCGAGCGGTTCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:3843982/71‑1 (MQ=255) tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt < 1:3039922/70‑1 (MQ=255) cgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa < 1:201995/68‑1 (MQ=255) ggCGCCCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcgt > 1:2612787/1‑52 (MQ=255) gCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAAt < 1:1605920/70‑1 (MQ=255) gACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:2757191/56‑1 (MQ=255) ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:1070637/1‑55 (MQ=255) ccTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc > 1:2224999/1‑56 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:1070854/1‑54 (MQ=255) cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg > 1:2985414/1‑71 (MQ=255) ccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg < 1:1017280/71‑1 (MQ=255) cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:853566/1‑71 (MQ=255) gAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg < 1:325348/43‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:1439225/48‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:2819306/48‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:697619/1‑57 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:2295813/1‑57 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctg > 1:727784/1‑68 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg > 1:1832239/1‑71 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3693529/71‑1 (MQ=255) aGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg > 1:1429452/1‑71 (MQ=255) cGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTa < 1:1031246/71‑1 (MQ=255) cGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGTGGCTGGCGtt < 1:3105730/71‑2 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:2986992/50‑1 (MQ=255) tGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3485686/66‑1 (MQ=255) gCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg > 1:3744958/1‑71 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt > 1:578630/1‑37 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGAt > 1:3754100/1‑37 (MQ=255) cGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTAtgtg < 1:3394863/70‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATGGACCAGGATAAATGCCGt < 1:4032851/53‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg < 1:2983292/54‑1 (MQ=255) tttACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATc < 1:1977388/71‑1 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGa > 1:4123587/1‑35 (MQ=255) tACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:1857657/1‑71 (MQ=255) tACAAGAGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCa > 1:539242/1‑70 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc > 1:1667466/1‑54 (MQ=255) tACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc > 1:2352836/1‑54 (MQ=255) aCAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACt > 1:2751332/1‑71 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2122629/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2085022/71‑1 (MQ=255) cAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1445361/71‑1 (MQ=255) cAAGCGCGAGGAGGACGGCATCGTCCTGATCGACCAGGACAAGTGCCGCGGCTGGCGGATGTGCa < 1:1434330/65‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATa < 1:1203018/40‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:3258100/71‑1 (MQ=255) aaGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg < 1:770933/71‑1 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:3891022/1‑40 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:3903465/1‑40 (MQ=255) gCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3126887/54‑1 (MQ=255) cGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:1464355/48‑1 (MQ=255) cGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCg < 1:3506649/53‑1 (MQ=255) tGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGt < 1:917889/52‑1 (MQ=255) aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGc < 1:1409670/56‑1 (MQ=255) aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGccc > 1:836903/1‑70 (MQ=255) aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg > 1:2578843/1‑71 (MQ=255) aagaagATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCg > 1:1113775/1‑71 (MQ=255) gaagaTGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:328541/1‑45 (MQ=255) gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTgg > 1:166444/1‑59 (MQ=255) gaTGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACa > 1:3400660/1‑70 (MQ=255) aTGGGATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:1931453/57‑1 (MQ=255) ggCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTg < 1:2478135/55‑1 (MQ=255) gCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCAc > 1:1487657/1‑52 (MQ=255) cATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACaaaaaaa > 1:3841752/1‑71 (MQ=255) tCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATc < 1:1790183/71‑1 (MQ=255) | TGCCTGAACCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGGCGTATGTGCATCACTGGATGCCCGTACAAAAAAATC > minE/835841‑835978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |