| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 190,114 | T→C | 14.3% | F537F (TTT→TTC) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 190,114 | 0 | T | C | 14.3% | 195.5 / 22.9 | 77 | F537F (TTT→TTC) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (37/29); new base C (6/5); total (43/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCT > minE/190046‑190180 | gCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAg < 1:738377/71‑1 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:989448/1‑70 (MQ=255) ataccTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAt < 1:1156892/66‑1 (MQ=255) ccTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCa > 1:2442605/1‑71 (MQ=255) cTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCAt > 1:3512026/1‑71 (MQ=255) cTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCAt > 1:3564507/1‑71 (MQ=255) tCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:3450749/1‑71 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc < 1:1918597/69‑1 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:1369840/1‑71 (MQ=255) gaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAg < 1:178726/56‑1 (MQ=255) cGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaaa < 1:2821963/71‑2 (MQ=255) gTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGt < 1:2450675/53‑1 (MQ=255) gggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTa > 1:244781/1‑49 (MQ=255) gggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:3267638/1‑63 (MQ=255) ggcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa > 1:3703444/4‑70 (MQ=255) ggcaTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa > 1:1480316/4‑70 (MQ=255) ggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt > 1:3199325/1‑52 (MQ=255) ggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa > 1:1260951/1‑66 (MQ=255) tCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCa < 1:3279586/71‑1 (MQ=255) cGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:4054129/1‑71 (MQ=255) tagtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGt < 1:3837901/44‑1 (MQ=255) agtagAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:4042529/1‑55 (MQ=255) gagaAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:1978557/51‑1 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCttt < 1:2328990/35‑1 (MQ=255) aaaaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:1400862/48‑1 (MQ=255) aaaaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:123114/48‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGattatt > 1:3405240/1‑71 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGattatt > 1:2056157/1‑71 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGattat > 1:1062013/1‑70 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatta > 1:3646824/1‑69 (MQ=255) aaaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:793907/45‑1 (MQ=255) aaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTg > 1:3524962/1‑70 (MQ=255) cGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:1231794/71‑1 (MQ=255) ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa < 1:3204363/52‑1 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa > 1:1858961/1‑42 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaaa > 1:454097/1‑42 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat < 1:1104122/57‑1 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat < 1:528183/57‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaa < 1:601685/39‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa < 1:2296245/44‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa < 1:526092/44‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTg < 1:2585473/61‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTg < 1:413607/61‑1 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGATaa > 1:3303219/1‑38 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAAcc < 1:1308194/42‑1 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3672948/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:634149/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:494207/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3735814/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:1081613/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:1326514/1‑71 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:1787046/1‑71 (MQ=255) ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:2876014/1‑47 (MQ=255) ataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:465623/1‑47 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa < 1:1272730/41‑1 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTggg > 1:2022705/1‑64 (MQ=255) aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGattat > 1:2867743/1‑54 (MQ=255) aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTggg > 1:4085314/1‑62 (MQ=255) ccTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAAt < 1:1704434/71‑1 (MQ=255) ctgctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCGATGGGATTATTGCGTGGGTTGACGGAAtt > 1:2887998/1‑71 (MQ=255) gctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat < 1:2068007/47‑1 (MQ=255) gctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGa > 1:2560406/1‑66 (MQ=255) tgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat < 1:2279967/45‑1 (MQ=255) gTTTCTCTTTAGTATTGGCATCGATAAAACCATAGc < 1:1995760/36‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAATCAAAGc < 1:3519/35‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:631527/35‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGc < 1:418897/35‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa < 1:2391942/37‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATgg < 1:1260194/40‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatt < 1:1382404/44‑1 (MQ=255) tttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg < 1:2674286/60‑1 (MQ=255) ttCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:2877912/1‑36 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATg > 1:1369305/1‑37 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatta > 1:1219563/1‑43 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGa > 1:513882/1‑56 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:3164196/1‑70 (MQ=255) tctcTTTAGTATTGGCATCGAAAAAGCCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGct > 1:1624280/1‑71 (MQ=255) tctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGctct > 1:3296535/1‑71 (MQ=255) tttAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGctct < 1:1242821/69‑1 (MQ=255) tAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTg < 1:3907176/49‑1 (MQ=255) | GCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGCTCT > minE/190046‑190180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |