| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 836,443 | A→G | 6.4% | V387V (GTA→GTG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 836,443 | 0 | A | G | 6.4% | 177.8 / 3.7 | 62 | V387V (GTA→GTG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base A (28/30); new base G (2/2); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
TGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTG > minE/836374‑836512 | tGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTa > 1:534009/1‑70 (MQ=255)tGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTa > 1:2159994/1‑70 (MQ=255) cagcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTa < 1:993127/68‑1 (MQ=255) cagcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACa < 1:4062474/70‑1 (MQ=255) cagcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACa < 1:2608575/70‑1 (MQ=255) cagcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAg < 1:460520/71‑1 (MQ=255) cagcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAg > 1:3973951/1‑71 (MQ=255) agcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGt < 1:3938332/71‑1 (MQ=255) agcagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGt < 1:3523021/71‑1 (MQ=255) cagACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTa < 1:1149689/70‑1 (MQ=255) cgcgGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTgg > 1:2122209/1‑71 (MQ=255) gcgGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGc > 1:3698168/1‑71 (MQ=255) gggTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCg > 1:3883790/1‑70 (MQ=255) gggTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGa < 1:2927223/71‑1 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctgctg < 1:1047054/69‑1 (MQ=255) ggTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctgctg < 1:1042115/69‑1 (MQ=255) tAGCAACGGCATTCTGCCAGACGTAGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATc > 1:2414971/1‑62 (MQ=255) gCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctg < 1:3048575/62‑1 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAAt > 1:3933056/1‑57 (MQ=255) aaCGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAAt > 1:3515285/1‑57 (MQ=255) cgtcATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTg < 1:3695159/68‑1 (MQ=255) ggCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGa < 1:2825019/71‑1 (MQ=255) ggCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGa < 1:3049400/71‑1 (MQ=255) cATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATa > 1:324108/1‑71 (MQ=255) ttCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGt < 1:27138/39‑1 (MQ=255) cTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTg > 1:2542316/1‑64 (MQ=255) cTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCa > 1:1357481/1‑70 (MQ=255) tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAc > 1:1358649/1‑67 (MQ=255) tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa > 1:1501839/1‑71 (MQ=255) ccAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc < 1:763241/70‑1 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCt > 1:1184506/1‑36 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt > 1:2182173/1‑58 (MQ=255) aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:3690589/71‑1 (MQ=255) aCGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAAtcttct < 1:1505454/46‑1 (MQ=255) tCGAAAGCCTGTGTATTCCGGTGCAATATCTCGCCaa < 1:759919/37‑1 (MQ=16) tCGAAAGCCTGCGTATTCCGGTGCAATATCTCGCCaa < 1:468029/37‑1 (MQ=21) tCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGt < 1:900946/53‑1 (MQ=255) gAAAGCCTGCGTATTCCGGTGCAATATCTCGCAAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTgcg > 1:305060/1‑71 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGaa > 1:1727636/1‑35 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctgctg > 1:2621622/1‑42 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATctg < 1:573089/39‑1 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc < 1:599095/48‑1 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc < 1:594163/48‑1 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAc > 1:2178356/1‑56 (MQ=255) gAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcg > 1:1760449/1‑71 (MQ=255) aaGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGAccgcc > 1:3962629/1‑46 (MQ=255) aaGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:70665/1‑71 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcgc < 1:2966477/71‑1 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg > 1:3622679/1‑70 (MQ=255) aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGATGATACCAAACCGGTACTgcgcg > 1:3739535/1‑70 (MQ=255) ccTGCGTATTCCGGTACAGTATCTGGCGAATCTGGTGACCGCCGGTGATACCAAACCGGTACTGCGCGCAc < 1:60223/71‑1 (MQ=255) cTGCGTATTACGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt < 1:2159068/71‑1 (MQ=255) gCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTg > 1:1951945/1‑70 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa > 1:3933348/1‑51 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:1218914/55‑1 (MQ=255) cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg < 1:3096074/55‑1 (MQ=255) ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgc > 1:840668/1‑59 (MQ=255) ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg > 1:578007/1‑70 (MQ=255) ttCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACg > 1:1156982/1‑70 (MQ=255) ccGGTACAGTATCTGGCGCATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa > 1:1809069/1‑70 (MQ=255) ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATAc > 1:175459/1‑41 (MQ=255) ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc < 1:412767/71‑1 (MQ=255) ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc < 1:36380/71‑1 (MQ=255) ggTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGc < 1:172290/71‑1 (MQ=255) tACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTg < 1:632821/71‑1 (MQ=255) | TGCAGCAGACGCGGGTGAGCTGGGTAGCAACGGCATTCTGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTG > minE/836374‑836512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |