| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 837,066 | A→G | 9.5% | E83E (GAA→GAG) | narJ → | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 837,066 | 0 | A | G | 9.5% | 112.6 / 4.4 | 42 | E83E (GAA→GAG) | narJ | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Reads supporting (aligned to +/- strand): ref base A (20/18); new base G (2/2); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
TACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACA > minE/837001‑837136 | tACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATccc > 1:3704445/1‑70 (MQ=255)tACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcg > 1:1823996/1‑71 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:1710408/70‑1 (MQ=255) cAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc > 1:1744669/1‑70 (MQ=255) cGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgc < 1:3057701/67‑1 (MQ=255) cGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAcc > 1:160268/1‑71 (MQ=255) cGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAc > 1:2800016/1‑70 (MQ=255) cGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAc > 1:2736397/1‑70 (MQ=255) aCTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGAc > 1:1340696/1‑67 (MQ=255) aCTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATCTACACGGCGAATCCCGCGAc > 1:528105/1‑67 (MQ=255) cGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCcgcg < 1:404767/55‑1 (MQ=255) ccGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg < 1:3914957/71‑1 (MQ=255) cgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGa < 1:23359/71‑1 (MQ=255) gcCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACct < 1:2252290/71‑1 (MQ=255) accACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACct > 1:857270/2‑71 (MQ=255) aCGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc > 1:46547/1‑70 (MQ=255) cGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGCACctgctg < 1:3545037/71‑1 (MQ=255) gTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTgg < 1:3787014/71‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg < 1:595993/70‑1 (MQ=255) aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg < 1:3956713/70‑1 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg > 1:3776751/1‑70 (MQ=255) gTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg > 1:1805778/1‑70 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtgg < 1:2545857/52‑1 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtgg < 1:242214/52‑1 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGAcc < 1:1417426/58‑1 (MQ=255) ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTa > 1:3485711/1‑71 (MQ=255) tttGAACATGTGCACGGCGAATCCCGCGACCGCGGc < 1:362568/36‑1 (MQ=255) ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGa > 1:3951436/1‑42 (MQ=255) tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc > 1:2153105/1‑39 (MQ=255) tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtg > 1:1407091/1‑46 (MQ=255) tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag < 1:923048/70‑1 (MQ=255) aCATGTGCACGGCGAATCCCGCGACCGCGTCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagca > 1:3913568/1‑66 (MQ=255) aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtgg > 1:3273629/1‑41 (MQ=255) aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtgg > 1:4076199/1‑41 (MQ=255) cATGTGCACGGCGAATCCCGCGACCGCTGCCAGGc < 1:1895560/35‑1 (MQ=255) aTGTGCACGGCGAATCCCGCGACCTCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGc < 1:3654186/71‑1 (MQ=255) tgtgCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCt > 1:3275405/1‑71 (MQ=255) gtgCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCtt > 1:3032360/1‑71 (MQ=255) gCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTa < 1:2712548/71‑1 (MQ=255) cGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg < 1:4042621/50‑1 (MQ=255) cGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAg > 1:1611202/1‑67 (MQ=255) cGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTaa < 1:2757249/71‑1 (MQ=255) aTCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACa < 1:1775296/71‑1 (MQ=255) | TACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACA > minE/837001‑837136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |