Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	854,882	A→G	11.5%	D36D (GAT→GAC)	yciG ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	854,882	0	A	G	11.5%	140.3 / 10.0	52	D36D (GAT→GAC)	yciG	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/27);  new base G (2/4);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

AGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCAC  >  minE/854812‑854946                                                                       |                                                                 aGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGa                                                                  <  1:2826311/71‑1 (MQ=255)            gATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                        >  1:3311015/1‑70 (MQ=255)             aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaaaa                                                      >  1:2608063/1‑71 (MQ=255)             aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaaaa                                                      <  1:1728286/71‑1 (MQ=255)             aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                        <  1:1100104/69‑1 (MQ=255)             aTTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                        <  1:3055749/69‑1 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGAt                                                                 >  1:2845877/1‑59 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGAt                                                                 >  1:1897317/1‑59 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     >  1:2845390/1‑71 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     >  1:2621739/1‑71 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     <  1:775819/71‑1 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     >  1:880963/1‑71 (MQ=255)              tttATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     <  1:1195799/71‑1 (MQ=255)               ttattaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     >  1:3186597/1‑70 (MQ=255)               ttattaCCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     >  1:2959527/1‑70 (MQ=255)                     ccaccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgcc                                              >  1:3602794/1‑71 (MQ=255)                       accGCTTTTTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACcgccgc                                            <  1:2905950/71‑1 (MQ=255)                        ccGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACcgccgc                                            >  1:1423511/1‑70 (MQ=255)                          gCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAtt                                                             >  1:1558315/1‑51 (MQ=255)                          gCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc                                                  <  1:1423181/62‑1 (MQ=255)                           cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccg                                                >  1:3628490/1‑63 (MQ=255)                           cTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccg                                                >  1:976829/1‑63 (MQ=255)                              tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCa                                                               >  1:2943820/1‑45 (MQ=255)                              tgttgACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaa                                                        <  1:3636562/52‑1 (MQ=255)                                 ttaccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAAt                                                     >  1:1305664/3‑52 (MQ=255)                                 ttaccaccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAAt                                                     >  1:3810581/3‑52 (MQ=255)                                      gCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                             <  1:1599748/71‑1 (MQ=255)                                       ccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttt                            <  1:3075387/71‑1 (MQ=255)                                       ccTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCttt                            <  1:4034793/71‑1 (MQ=255)                                        cTTTTTTACCTGCTTCAGATGCGCGCTGCGGGTCAtt                                                             <  1:930354/37‑1 (MQ=25)                                        cTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAtt                                                    <  1:3144155/46‑1 (MQ=255)                                         ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAt                                                     <  1:2300730/44‑1 (MQ=255)                                         ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTAt                                         <  1:335368/56‑1 (MQ=255)                                         ttttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTAt                                         <  1:2961839/56‑1 (MQ=255)                                           ttttACCTGCTTCAGATGCGCGCTGCGGGTCATTCTTGAAATTACCCCCGCTGTGCTGTCCACCtttttt                         <  1:1365589/70‑4 (MQ=255)                                           ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTAc                          <  1:2023585/69‑1 (MQ=255)                                           ttttACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACgg                        >  1:3378161/1‑71 (MQ=255)                                                    cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCg               <  1:64729/71‑1 (MQ=255)                                                    cTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCg               <  1:3970837/71‑1 (MQ=255)                                                      tCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGa             >  1:854799/1‑71 (MQ=255)                                                        aGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATg           <  1:1524835/71‑1 (MQ=255)                                                         gATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTATGGCCTGCGTCGGATGc          <  1:3409029/71‑1 (MQ=255)                                                          aTGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGcc         >  1:834722/1‑71 (MQ=255)                                                            gcgcGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCg                  <  1:2461211/60‑1 (MQ=255)                                                            gcgcGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCtt       >  1:3489900/1‑71 (MQ=255)                                                             cgcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCtt       <  1:3442763/70‑1 (MQ=255)                                                              gcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCtt       <  1:3753653/69‑1 (MQ=255)                                                              gcgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTtct     <  1:2298393/71‑1 (MQ=255)                                                               cgTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTtctc    <  1:3162706/71‑1 (MQ=255)                                                                 ttGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCAc  <  1:1642648/71‑1 (MQ=255)                                                                 ttGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCAc  <  1:1705479/71‑1 (MQ=255)                                                                     ggATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCtt                             <  1:27010/40‑1 (MQ=255)                                                                      gATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCg               <  1:319862/53‑1 (MQ=255)                                                                       |                                                                 AGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATGCTGACCGCCTTTACGGCCTGCGTCGGATGCCTTCTCAC  >  minE/854812‑854946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑