| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 873,015 | C→T | 30.3% | I69I (ATC→ATT) | cysB → | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 873,015 | 0 | C | T | 30.3% | 79.3 / 43.7 | 56 | I69I (ATC→ATT) | cysB | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (17/22); new base T (7/10); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAA > minE/872946‑873083 | tCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATTATTCGTAtcg > 1:2105704/1‑71 (MQ=255)tCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcg > 1:1709125/1‑71 (MQ=255) cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAttgc > 1:1667958/1‑71 (MQ=255) cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgc > 1:794516/1‑71 (MQ=255) cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcg > 1:1514030/1‑70 (MQ=255) cAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtc > 1:1363998/1‑69 (MQ=255) aaaTTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAtcgc < 1:466711/70‑1 (MQ=255) ttttCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTcgcg < 1:3943066/70‑1 (MQ=255) ttttCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTcgcg < 1:24293/70‑1 (MQ=255) ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTc < 1:884551/70‑1 (MQ=255) ccGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTc < 1:879726/70‑1 (MQ=255) cGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGa < 1:947184/71‑1 (MQ=255) cGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGa < 1:111638/71‑1 (MQ=255) aGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTAttgct < 1:1648449/50‑1 (MQ=255) cTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCCCTCGCGAAGTCCTGTCGAAAGTc < 1:2825312/66‑1 (MQ=255) aCGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa > 1:3260612/1‑70 (MQ=255) aCGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCa > 1:2967452/1‑70 (MQ=255) ggTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc < 1:2098486/58‑1 (MQ=255) gTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGa < 1:944225/59‑1 (MQ=255) tAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCg < 1:1674500/71‑1 (MQ=255) aaCGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGACATAAAATCgg > 1:3835958/1‑71 (MQ=255) aCGCCAGCAGGGCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGaaa > 1:1594703/1‑51 (MQ=255) aCGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCgg < 1:2754123/70‑1 (MQ=255) aCGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCgg < 1:3809027/70‑1 (MQ=255) aCGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGt > 1:1868819/1‑71 (MQ=255) gCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc > 1:72425/1‑52 (MQ=255) gCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGtt > 1:2038126/1‑70 (MQ=255) ccAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc < 1:2119143/51‑1 (MQ=255) ccAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGc < 1:3045436/71‑1 (MQ=255) gcagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCgg < 1:430252/71‑1 (MQ=255) cagGGCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTc > 1:4038286/1‑47 (MQ=255) cagGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGga < 1:596526/71‑1 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc > 1:1238861/1‑46 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc > 1:3303602/1‑46 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg > 1:1983628/1‑68 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg > 1:3022360/1‑68 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg > 1:2713215/1‑68 (MQ=255) agGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg > 1:2282388/1‑68 (MQ=255) ggCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCg < 1:172852/59‑1 (MQ=255) ggCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGagag < 1:2005824/71‑1 (MQ=255) ggCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCg < 1:2786212/59‑1 (MQ=255) gCAAGAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCg < 1:2601727/58‑1 (MQ=255) gCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCgg < 1:1808797/59‑1 (MQ=255) cAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCAt < 1:3055406/50‑1 (MQ=255) cAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca > 1:108173/1‑71 (MQ=255) cAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca > 1:3681028/1‑71 (MQ=255) cAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca > 1:2512159/1‑71 (MQ=255) cAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAAT‑GGTTGCCGGAGAGcac < 1:3684888/71‑1 (MQ=255) aGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCg < 1:3184731/62‑1 (MQ=255) gAAATAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTg < 1:894703/58‑1 (MQ=255) aaaTAATTCTTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGtt > 1:695057/1‑56 (MQ=255) aaaTAATTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGcaca > 1:2916832/1‑69 (MQ=255) aataatTCGTATCGCTCGCGAAGTCCTGTCGAAAGTc > 1:3793319/1‑37 (MQ=255) ataatTCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTg < 1:620697/71‑1 (MQ=255) ataatTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTg > 1:3494211/1‑71 (MQ=255) ataatTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTg > 1:1835544/1‑71 (MQ=255) ttCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc < 1:1985964/46‑1 (MQ=255) ttCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc < 1:3850128/46‑1 (MQ=255) ttCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATc < 1:2189846/46‑1 (MQ=255) ttCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTTCCGGAGAGCACACCTGGCCg < 1:1022593/71‑1 (MQ=255) tCGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCg > 1:3148573/1‑70 (MQ=255) cGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCgg > 1:3757235/1‑46 (MQ=255) cGTATTGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGgag < 1:263576/55‑1 (MQ=255) cGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGca < 1:3736748/59‑1 (MQ=255) tcgctcgcGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATaa < 1:2475068/70‑1 (MQ=255) | TCAAATTTTTTCCCGAAGCGGCAAGCACCTGACGCAGGTAACGCCAGCAGGGCAAGAAATAATTCGTATCGCTCGCGAAGTCCTGTCGAAAGTCGATGCCATAAAATCGGTTGCCGGAGAGCACACCTGGCCGGATAA > minE/872946‑873083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |