| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 909,663 | A→G | 46.6% | I614V (ATC→GTC) | ycjT → | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 909,663 | 0 | A | G | 46.6% | 5.6 / 80.2 | 58 | I614V (ATC→GTC) | ycjT | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (18/13); new base G (14/13); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAGGGGAC > minE/909596‑909730 | aTCGTGTCTTGCCAATCTGCATTTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTc < 1:593456/70‑1 (MQ=255) tCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCa < 1:1494266/71‑1 (MQ=255) gtgtCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACg > 1:3081615/1‑71 (MQ=255) tgtCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACgg < 1:3918250/71‑1 (MQ=255) ttGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAtt > 1:379199/1‑71 (MQ=255) ttGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAt > 1:1087167/1‑70 (MQ=255) gCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAttgt > 1:1859680/1‑71 (MQ=255) gCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAt < 1:1016130/68‑1 (MQ=255) tGCAATTTTATGAACCGCTCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACg > 1:2591768/1‑57 (MQ=255) tGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACg > 1:3118027/1‑57 (MQ=255) tGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACg > 1:3556163/1‑57 (MQ=255) aaTTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCg < 1:348424/66‑1 (MQ=255) ttatATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCtg > 1:1941286/4‑71 (MQ=255) tttATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCtgt > 1:3713370/1‑71 (MQ=255) ttATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCtgtg > 1:476332/1‑71 (MQ=255) ttATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCtgtg > 1:755221/1‑71 (MQ=255) tATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCtgtg < 1:3564915/70‑1 (MQ=255) aCCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAttgt > 1:2340308/1‑51 (MQ=255) aCCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCAttgt > 1:1645990/1‑51 (MQ=255) aCCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCctgc > 1:1714678/1‑71 (MQ=255) aCCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctg < 1:3376218/70‑1 (MQ=255) cgcgCACTATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCctgct < 1:3891500/70‑1 (MQ=255) cACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCct < 1:626519/63‑1 (MQ=255) aCTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCAtt < 1:1400316/42‑1 (MQ=255) aCTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctg < 1:1239426/63‑1 (MQ=255) tATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCtg < 1:1496020/54‑1 (MQ=255) tATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCt < 1:4086859/53‑1 (MQ=255) tATTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCt < 1:3699642/53‑1 (MQ=255) tATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCAttgtt < 1:23556/43‑1 (MQ=255) aTTCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGc < 1:1375207/51‑1 (MQ=255) aTTCACGACTCGTCATTAAGTAAAGCAATCCACGGCAttgtt < 1:2592789/42‑1 (MQ=255) aTTCACGACTCGTCATTAAGTAAAGCAATCCACGGCAttgtt < 1:1814806/42‑1 (MQ=255) aTTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAg > 1:771028/1‑71 (MQ=255) aTTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAg > 1:849353/1‑71 (MQ=255) ttCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCtgt > 1:3415257/1‑53 (MQ=255) ttCACGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGCCCCAAAGt > 1:3879099/1‑71 (MQ=255) ttCACGACTCGTCATTAAGTAAAGCAATCCACGGCAt > 1:3185560/1‑37 (MQ=255) tCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGa < 1:1367784/62‑1 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctgct < 1:624701/59‑1 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctgc > 1:3280730/1‑58 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctgc > 1:3050673/1‑58 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCctgc > 1:462422/1‑58 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTa > 1:713545/1‑71 (MQ=255) cACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTa > 1:2802119/1‑71 (MQ=255) aCGACTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTAt > 1:2525375/1‑71 (MQ=255) aCGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTAt > 1:3082669/1‑71 (MQ=255) cGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCg < 1:3582292/41‑1 (MQ=255) gACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTAt > 1:3927270/1‑69 (MQ=255) aCTCGTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAg > 1:1402486/1‑71 (MQ=255) gTCATTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCt > 1:2800658/1‑71 (MQ=255) gTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCt > 1:3662017/1‑71 (MQ=255) aTTAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTgg < 1:2836073/70‑1 (MQ=255) tAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCt < 1:599457/36‑1 (MQ=255) tAAGTAAAGCAGTCCACGGCATTGTTGCCGCACGCt < 1:1663665/36‑1 (MQ=255) gTAAAGCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAg < 1:1793540/71‑1 (MQ=255) gTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCCAg > 1:1863911/1‑71 (MQ=255) aaaGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTg > 1:3870361/1‑62 (MQ=255) gCAGTCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAGGGGAc < 1:2281977/71‑1 (MQ=255) | ATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACGACTCGTCATTAAGTAAAGCAATCCACGGCATTGTTGCCGCACGCTGTGGCCTGCTGACCCAAAGTTATCAGTTCTGGCGCGAGGGGAC > minE/909596‑909730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |