Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 53,515 G→A 14.1% P425S (CCT→TCT)  imp ← exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE53,5150GA14.1% 207.2 / 22.6 78P425S (CCT→TCT) impexported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand):  ref base G (37/30);  new base A (4/7);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

TTGATGCTGCCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGACCAACAT  >  minE/53445‑53583
                                                                      |                                                                    
ttGATGCTGCCCCACTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAgg                                                                      <  1:494116/71‑1 (MQ=255)
  gATGCTGCCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCa                                                                    >  1:3655751/1‑71 (MQ=255)
  gATGCTGCCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCa                                                                    >  1:1777816/1‑71 (MQ=255)
  gATGCTGCCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACa                                                                    >  1:714772/1‑71 (MQ=255)
    tgctgcCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATg                                                                  >  1:3426263/1‑71 (MQ=255)
     gctgcCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCa                                                                    >  1:947537/1‑68 (MQ=255)
       tgcCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATgtc                                                                <  1:99404/70‑1 (MQ=255)
        gcCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATgtcgt                                                              >  1:855738/1‑71 (MQ=255)
           ccAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGc                                                                     >  1:72654/1‑61 (MQ=255)
              gTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTg                                                         >  1:3396313/1‑70 (MQ=255)
              gTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTgg                                                        >  1:3172069/1‑71 (MQ=255)
                tattaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGgtg                                                      >  1:286557/1‑71 (MQ=255)
                tattaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGgtg                                                      >  1:2432499/1‑71 (MQ=255)
                 attaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   >  1:3261323/1‑57 (MQ=255)
                 attaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   >  1:2645327/1‑57 (MQ=255)
                 attaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   >  1:3121894/1‑57 (MQ=255)
                 attaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   >  1:3084994/1‑57 (MQ=255)
                 attaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGgtgt                                                     >  1:625163/1‑71 (MQ=255)
                  ttaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAgg                                                                      <  1:3250178/53‑1 (MQ=255)
                  ttaGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGgtgt                                                     >  1:1991566/1‑70 (MQ=255)
                   taGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACAAGGGTTGCTTCAGGCATGTCGTCTCTGGTGtt                                                    <  1:2341335/70‑1 (MQ=255)
                    agagagCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAgg                                                                      <  1:1521729/51‑1 (MQ=255)
                     gagagCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTATGGTGTTaa                                                  >  1:3326479/1‑70 (MQ=255)
                      agagCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACa                                                <  1:281175/71‑1 (MQ=255)
                           ggCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTg                                           <  1:2262127/71‑1 (MQ=255)
                              aaaTTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCAc                                        >  1:3398919/1‑71 (MQ=255)
                               aaTTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACt                                       <  1:347846/71‑1 (MQ=255)
                               aaTTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACa                                                <  1:3663895/62‑1 (MQ=255)
                               aaTTGATGGTCGGTTCCAGGTGAACACGGGGTGCTTCAGGCATGTCGTCTCTGGTGTTAAc                                                 <  1:1570910/61‑1 (MQ=255)
                                aTTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   <  1:1002258/42‑1 (MQ=255)
                                aTTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   <  1:3412005/42‑1 (MQ=255)
                                 ttGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCa                                                                    >  1:395176/1‑40 (MQ=255)
                                 ttGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCAt                                                                   >  1:2530872/1‑41 (MQ=255)
                                 ttGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGc                                     >  1:2703610/1‑71 (MQ=255)
                                 ttGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGc                                     >  1:1370351/1‑71 (MQ=255)
                                 ttGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACATgtcg                                                               <  1:1054841/45‑1 (MQ=255)
                                  tGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCa                                                                    >  1:1152409/1‑39 (MQ=255)
                                   gATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATgt                                                                 >  1:1443150/1‑41 (MQ=255)
                                   gATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCt                                   >  1:3234593/1‑71 (MQ=255)
                                     tGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTgg                                 >  1:2221387/1‑71 (MQ=255)
                                      ggTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATgtcgtc                                                             <  1:2375522/42‑1 (MQ=255)
                                      ggTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATgtcgtc                                                             <  1:2856683/42‑1 (MQ=255)
                                        tCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCg                              >  1:2025529/1‑71 (MQ=255)
                                          ggTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGc                                >  1:3020442/1‑67 (MQ=255)
                                          ggTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGt                             >  1:2946302/1‑70 (MQ=255)
                                          ggTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTa                            <  1:2682732/71‑1 (MQ=255)
                                           gTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACa                                                <  1:1027190/50‑1 (MQ=255)
                                            ttCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTaaa                          <  1:4085967/71‑1 (MQ=255)
                                               cAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATa                        <  1:1076992/70‑1 (MQ=255)
                                               cAGGTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATa                        <  1:2344450/70‑1 (MQ=255)
                                                aGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACaa                                               <  1:2050457/46‑1 (MQ=255)
                                                aGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACaa                                               <  1:1546078/46‑1 (MQ=255)
                                                aGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATAcg                      <  1:3931441/71‑1 (MQ=255)
                                                aGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATAcg                      <  1:750409/71‑1 (MQ=255)
                                                  gTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAAc                                                 <  1:3202896/42‑1 (MQ=255)
                                                  gTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACa                                                <  1:1750192/43‑1 (MQ=255)
                                                  gTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATa                        >  1:1034356/1‑67 (MQ=255)
                                                  gTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATAcgcg                    <  1:1673466/71‑1 (MQ=255)
                                                  gTGAACACGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATAcgcg                    <  1:2520563/71‑1 (MQ=255)
                                                   tGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCa                                         >  1:1627182/1‑49 (MQ=255)
                                                   tGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCg                              >  1:2309830/1‑60 (MQ=255)
                                                    gAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGt                                            >  1:4027952/1‑45 (MQ=255)
                                                    gAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTa                  >  1:3457132/1‑71 (MQ=255)
                                                    gAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTa                  >  1:3860420/1‑71 (MQ=255)
                                                     aaCACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGt                   <  1:3893572/69‑1 (MQ=255)
                                                     aaCACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGt                   <  1:1735804/69‑1 (MQ=255)
                                                       cacGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGt                             <  1:686763/57‑1 (MQ=255)
                                                       cacGGGTTGCTTCAGACATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCa               <  1:839032/71‑1 (MQ=255)
                                                        acGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTg                                           <  1:3336692/42‑1 (MQ=255)
                                                         cGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAg                                             >  1:626031/1‑39 (MQ=255)
                                                          gggTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAAc            <  1:2836641/71‑1 (MQ=255)
                                                             ttGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTaaa                          <  1:3252662/54‑1 (MQ=255)
                                                             ttGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGt                   <  1:2700549/61‑1 (MQ=255)
                                                                cTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGAcc       <  1:3078924/70‑1 (MQ=255)
                                                                cTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGAcc       <  1:2102830/70‑1 (MQ=255)
                                                                cTTCAGACATGTCGTCTCTGGTGTTTACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGAcc       <  1:1795556/70‑1 (MQ=255)
                                                                   cAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCt                                   >  1:1563564/1‑39 (MQ=255)
                                                                   cAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCaaa             >  1:3983619/1‑61 (MQ=255)
                                                                    aGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGa         >  1:961322/1‑64 (MQ=255)
                                                                    aGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGACCAAcat  >  1:1181744/1‑71 (MQ=255)
                                                                    aGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGACCAAc    <  1:3024239/69‑1 (MQ=255)
                                                                      |                                                                    
TTGATGCTGCCCCAGTTATTAGAGAGCGGCAAATTGATGGTCGGTTCCAGGTGAACACGGGTTGCTTCAGGCATGTCGTCTCTGGTGTTAACAAAGTGCACTGCCTGGCCGTAAATACGCGTATCAAACGGACCAACAT  >  minE/53445‑53583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: