| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,940 | T→C | 12.1% | D60D (GAT→GAC) | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,940 | 0 | T | C | 12.1% | 170.7 / 15.2 | 66 | D60D (GAT→GAC) | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (20/38); new base C (5/3); total (25/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAG > minE/910871‑911009 | accGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATc < 1:1267345/70‑1 (MQ=255) ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCt < 1:1181329/71‑1 (MQ=2) tctggCTGCGGTAAATCGCCTTTAATGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGCCCTGtt > 1:3183041/4‑71 (MQ=2) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3022653/70‑1 (MQ=255) gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:4011109/70‑1 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATc > 1:1992317/1‑71 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATc > 1:1274434/1‑71 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:3724427/1‑71 (MQ=255) gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:3202758/1‑69 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTg < 1:2326693/59‑1 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACgg > 1:213273/1‑70 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGc < 1:2877984/71‑1 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATc > 1:1641979/1‑54 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTg < 1:2261862/56‑1 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3968957/59‑1 (MQ=255) tCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:1871673/71‑1 (MQ=255) ccaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:3637183/71‑1 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatga < 1:3949279/67‑1 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:2784703/71‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3178361/49‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:1328184/70‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga < 1:3337133/71‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:819276/1‑71 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:1196501/1‑71 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:3636390/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt > 1:1972096/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt < 1:746103/71‑1 (MQ=255) cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGAcac > 1:232109/1‑69 (MQ=255) gcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:3754410/71‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatga < 1:2961061/61‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc > 1:1435543/1‑70 (MQ=255) ttGCCGGGCTTGAGGAGATCAGCGTCGGCGATCTg > 1:3106202/1‑35 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:1422315/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaa < 1:2407201/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaa < 1:1330503/70‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:3144211/46‑1 (MQ=255) cTTGATGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg < 1:1031705/67‑1 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:982836/69‑1 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa < 1:246438/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2992173/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2773716/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2769220/70‑1 (MQ=255) ggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata < 1:1932140/71‑1 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAatat < 1:3074734/71‑1 (MQ=255) agaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:3769610/1‑69 (MQ=255) gaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:1529971/49‑1 (MQ=255) gaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:1534919/49‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:503137/35‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc > 1:4091666/1‑71 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa > 1:2414120/1‑71 (MQ=255) cAGCGGAGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc > 1:160553/1‑69 (MQ=255) aGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCAc > 1:3388101/1‑58 (MQ=255) ggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgt > 1:3100401/1‑71 (MQ=255) gcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgt < 1:2941707/70‑1 (MQ=255) cggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt > 1:2418846/1‑71 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3243805/71‑1 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3927625/71‑1 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3990331/71‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:3073330/53‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgtac > 1:8839/1‑67 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc > 1:3521052/1‑69 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc > 1:1429221/1‑69 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa > 1:1208294/1‑44 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:301502/50‑1 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:312744/50‑1 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt < 1:3423740/66‑1 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCa > 1:4084645/1‑69 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCa > 1:293706/1‑69 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg > 1:575362/1‑70 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg > 1:1009892/1‑70 (MQ=255) | CCCGTCGGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAG > minE/910871‑911009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |