| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 910,946 | G→C | 8.2% | L62F (TTG→TTC) | ycjV → | predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 910,946 | 0 | G | C | 8.2% | 216.0 / 8.0 | 73 | L62F (TTG→TTC) | ycjV | predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (32/35); new base C (4/2); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACG > minE/910877‑911016 | gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:4011109/70‑1 (MQ=255)gggCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3022653/70‑1 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATc > 1:1274434/1‑71 (MQ=255) gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATc > 1:1992317/1‑71 (MQ=255) tGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGa > 1:3724427/1‑71 (MQ=255) gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCggt > 1:3202758/1‑69 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACgg > 1:213273/1‑70 (MQ=255) gTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGc < 1:2877984/71‑1 (MQ=255) aGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3968957/59‑1 (MQ=255) tCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:1871673/71‑1 (MQ=255) ccaccCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatg < 1:3637183/71‑1 (MQ=255) tacTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACggatga < 1:3949279/67‑1 (MQ=255) cccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:2784703/71‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTg < 1:3178361/49‑1 (MQ=255) ccTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatgaat < 1:1328184/70‑1 (MQ=255) cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGatgaatga < 1:3337133/71‑1 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:3636390/1‑71 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:819276/1‑71 (MQ=255) tGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGAc > 1:1196501/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt > 1:1972096/1‑71 (MQ=255) cgcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGt < 1:746103/71‑1 (MQ=255) cgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGAcac > 1:232109/1‑69 (MQ=255) gcATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:3754410/71‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACgaatga < 1:2961061/61‑1 (MQ=255) aTGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTc > 1:1435543/1‑70 (MQ=255) gCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:1422315/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaa < 1:1330503/70‑1 (MQ=255) ccGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaa < 1:2407201/70‑1 (MQ=255) ggCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:3144211/46‑1 (MQ=255) cTTGATGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAg < 1:1031705/67‑1 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:982836/69‑1 (MQ=255) gaggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCa < 1:246438/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2769220/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2773716/70‑1 (MQ=255) aggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:2992173/70‑1 (MQ=255) ggagATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata < 1:1932140/71‑1 (MQ=255) gagaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAatat < 1:3074734/71‑1 (MQ=255) agaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:3769610/1‑69 (MQ=255) gaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:1534919/49‑1 (MQ=255) gaTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGtt < 1:1529971/49‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACg < 1:503137/35‑1 (MQ=255) aTCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc > 1:4091666/1‑71 (MQ=255) cAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGa > 1:2414120/1‑71 (MQ=255) cAGCGGAGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc > 1:160553/1‑69 (MQ=255) aGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCAc > 1:3388101/1‑58 (MQ=255) ggcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgt > 1:3100401/1‑71 (MQ=255) gcggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgt < 1:2941707/70‑1 (MQ=255) cggcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt > 1:2418846/1‑71 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3927625/71‑1 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3990331/71‑1 (MQ=255) gcgATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc < 1:3243805/71‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCaa < 1:3073330/53‑1 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgtac > 1:8839/1‑67 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc > 1:3521052/1‑69 (MQ=255) gATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTc > 1:1429221/1‑69 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCaaa > 1:1208294/1‑44 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:312744/50‑1 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGc < 1:301502/50‑1 (MQ=255) aTCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtgt < 1:3423740/66‑1 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCa > 1:293706/1‑69 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCa > 1:4084645/1‑69 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg > 1:575362/1‑70 (MQ=255) tCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAg > 1:1009892/1‑70 (MQ=255) cTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:3565876/1‑52 (MQ=255) cTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGaa > 1:3912644/1‑71 (MQ=255) cTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGaa > 1:73728/1‑71 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:2870513/1‑51 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTcc > 1:1232187/1‑66 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:3246020/1‑71 (MQ=255) tGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc > 1:1041211/1‑71 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGc < 1:1237288/53‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGgtg > 1:3780583/1‑60 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGa < 1:3316595/67‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAAc < 1:1021650/69‑1 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTa > 1:394562/1‑71 (MQ=255) ttGATCGACGGCAAACGAATGAATGACGTTCCAGCAAAAGCACGCAATATAGCGAt < 1:2737563/56‑1 (MQ=255) tGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAc > 1:373944/1‑71 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCa < 1:2623738/35‑1 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAatat > 1:2104256/1‑48 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:2296967/1‑47 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAata > 1:995300/1‑47 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg > 1:2921120/1‑71 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg > 1:701210/1‑71 (MQ=255) gATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTAcg > 1:2475327/1‑71 (MQ=255) gATAGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAat > 1:361137/1‑46 (MQ=255) | GGGCTGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGATCTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATGGTGTTCCAGAACTACG > minE/910877‑911016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |