| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 962,083 | A→G | 14.9% | Y214H (TAT→CAT) | yddB ← | predicted porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 962,083 | 0 | A | G | 14.9% | 113.4 / 13.3 | 47 | Y214H (TAT→CAT) | yddB | predicted porin protein |
| Reads supporting (aligned to +/- strand): ref base A (20/20); new base G (3/4); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CCGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTC > minE/962013‑962152 | ccGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGtaata < 1:542812/71‑1 (MQ=255) aTCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTc > 1:247042/1‑71 (MQ=255) aTCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTc > 1:1873797/1‑71 (MQ=255) cAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAg < 1:102479/63‑1 (MQ=255) gAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTg > 1:781666/1‑68 (MQ=255) tCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCT‑GGGAGTAATAAGTACt < 1:3439859/58‑1 (MQ=255) aTTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTg < 1:3015785/69‑1 (MQ=255) aTTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTg < 1:232709/69‑1 (MQ=255) tAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGAtt > 1:2757902/1‑70 (MQ=255) aaaCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa < 1:1821132/70‑1 (MQ=255) aaaCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTa < 1:2651663/70‑1 (MQ=255) aaaCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:3874808/70‑1 (MQ=255) aaaCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:171206/70‑1 (MQ=255) aCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAAt > 1:3322525/1‑71 (MQ=255) aCGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAAt > 1:310723/1‑71 (MQ=255) aCAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGaa > 1:3016253/1‑53 (MQ=255) cAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATgcgc < 1:2895301/71‑1 (MQ=255) aaGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGa > 1:989676/1‑60 (MQ=255) tataAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:2344083/59‑1 (MQ=255) tataAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg > 1:428053/1‑71 (MQ=255) tataAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg > 1:2446321/1‑71 (MQ=255) ataAAAGTTCTTTTTTAAAATCTGGGGAGtaataa > 1:1419579/1‑35 (MQ=37) taAAAGTTC‑TTTTTAAAATCTGTGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt < 1:2267242/70‑1 (MQ=255) taAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCtt < 1:2081400/52‑1 (MQ=255) taAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCtt < 1:1431657/52‑1 (MQ=255) taAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGt < 1:2728543/70‑1 (MQ=255) aaaaGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa > 1:1655067/1‑71 (MQ=255) aaaGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTcc > 1:3553250/1‑39 (MQ=255) aaaGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg < 1:47239/67‑1 (MQ=255) aaaGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTg < 1:2075644/67‑1 (MQ=255) aaaGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt > 1:1026542/1‑71 (MQ=255) aaGTTC‑TTTTTAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGctc < 1:2092204/63‑1 (MQ=255) aaGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:297380/54‑1 (MQ=255) ttC‑TTTTTAAAATCTGTGGAGTAATAAGTACTTCCTGAAGAGCCt > 1:298437/1‑45 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:3825705/51‑1 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:1441772/51‑1 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTa < 1:1305307/51‑1 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTa > 1:1013502/1‑66 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtct > 1:2419448/1‑70 (MQ=255) ttC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtct > 1:3317894/1‑70 (MQ=255) c‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt > 1:545619/1‑65 (MQ=255) c‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATtctc > 1:2480418/1‑69 (MQ=255) ttttAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATc > 1:542814/1‑70 (MQ=255) tttAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGa > 1:2314621/1‑43 (MQ=255) ttAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGa > 1:3635648/1‑70 (MQ=255) tAAAATCTGGGGAGTAATGAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGata > 1:3794866/1‑71 (MQ=255) ggAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTa < 1:1550053/71‑1 (MQ=255) ggAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTa < 1:2227091/71‑1 (MQ=255) gTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAAc < 1:970114/70‑1 (MQ=255) taataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGatat < 1:3893663/58‑1 (MQ=255) taataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGa < 1:2273171/62‑1 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTAt > 1:1738452/1‑44 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATc < 1:1249327/51‑1 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGtt < 1:760143/64‑1 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt > 1:134550/1‑71 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt > 1:1233206/1‑71 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt > 1:1902873/1‑71 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt > 1:2277691/1‑71 (MQ=255) ataaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGt > 1:961091/1‑71 (MQ=255) taaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGtt < 1:3905293/41‑1 (MQ=255) taaGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTc > 1:2032144/1‑71 (MQ=255) | CCGAAGTTATCAGCGAGTTCCTGATTAAACGACAAGGTATAAAAGTTC‑TTTTTAAAATCTGGGGAGTAATAAGTACTTCCTGAAGAGCCTTGATTAAATGCGCTCTTGTTATTCTCATCGATATGCGATGTTAACCAGTC > minE/962013‑962152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |