| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 965,844 | A→T | 15.5% | V17D (GTT→GAT) | ydeM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 965,844 | 0 | A | T | 15.5% | 191.3 / 25.6 | 77 | V17D (GTT→GAT) | ydeM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (41/24); new base T (4/8); total (45/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGG > minE/965776‑965913 | tCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAaca > 1:3367171/1‑71 (MQ=255) cAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAtca < 1:2407403/70‑1 (MQ=255) cAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAaca > 1:425990/1‑70 (MQ=255) cTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTgag < 1:1215504/71‑1 (MQ=255) cTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTga < 1:2305268/70‑1 (MQ=255) cTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTga < 1:4082731/70‑1 (MQ=255) gTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATcaca < 1:765196/63‑1 (MQ=255) gTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACAttt < 1:622198/66‑1 (MQ=255) gTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTg > 1:1520676/1‑67 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTgaga > 1:1950539/1‑69 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGAtt < 1:2128243/71‑1 (MQ=255) tCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGAtt < 1:2133038/71‑1 (MQ=255) atccatccATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACAttt > 1:932266/1‑63 (MQ=255) atccatccATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTTAGATTAc < 1:3873208/71‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTGAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa > 1:321938/1‑70 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaa < 1:1600048/71‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:3968506/70‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa > 1:101026/1‑70 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:1865227/70‑1 (MQ=255) catTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:88770/70‑1 (MQ=255) atTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa < 1:412593/62‑1 (MQ=255) atTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTa < 1:1754669/62‑1 (MQ=255) atTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa < 1:3925720/71‑1 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGAt > 1:1751548/1‑59 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:3006077/68‑1 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAAc > 1:4084009/1‑71 (MQ=255) tttttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAAc < 1:1428919/71‑1 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:2757771/1‑68 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:2282056/1‑68 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:3840430/1‑68 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaa > 1:2286931/1‑67 (MQ=255) tttCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTg > 1:3457659/1‑71 (MQ=255) tCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTga < 1:1606441/52‑1 (MQ=255) aTGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaa < 1:1899852/63‑1 (MQ=255) gAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGc > 1:242664/1‑71 (MQ=255) aGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTaaa > 1:2854150/1‑38 (MQ=255) aGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTaaa > 1:69519/1‑38 (MQ=255) aGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAaca < 1:963551/40‑1 (MQ=255) aGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCt > 1:1453691/1‑71 (MQ=255) tAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTg < 1:897553/71‑1 (MQ=255) tAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTg > 1:3159773/1‑71 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGa > 1:780374/1‑62 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGa > 1:214944/1‑62 (MQ=255) aaCTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGc > 1:53371/1‑71 (MQ=255) cTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTg > 1:1767555/1‑71 (MQ=255) tGCGACTCTTTTTCAAGGTAAAAACAGTAAACACAtt > 1:1122556/1‑37 (MQ=255) tGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTg > 1:2617831/1‑70 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:1577470/1‑52 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTa < 1:2587788/70‑1 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTa < 1:879020/70‑1 (MQ=255) cGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTa > 1:147067/1‑70 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:1234498/1‑49 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:2212611/1‑49 (MQ=255) ctctTTTTCAAGGTAAAAACAGTAAACACATTTGAGA‑TACATTGAAAACTGGAGGGCTTGGCTGTAACGTg < 1:3053779/71‑1 (MQ=255) ctTTTTCAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTg > 1:1871060/1‑70 (MQ=255) ctTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACa > 1:2574246/1‑40 (MQ=255) tttttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaa < 1:3583908/44‑1 (MQ=255) ttttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGaaaa > 1:1586746/1‑45 (MQ=255) tttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATc > 1:3436399/1‑71 (MQ=255) ttCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCg > 1:3969067/1‑71 (MQ=255) cAAGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGc < 1:1293309/70‑1 (MQ=255) aaGGTAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCt < 1:1504517/70‑1 (MQ=255) ggTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTg > 1:1380069/1‑61 (MQ=255) ggTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtc > 1:478600/1‑71 (MQ=255) ggTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtc > 1:68863/1‑71 (MQ=255) ggTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtc > 1:2028062/1‑71 (MQ=255) gTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTg < 1:814012/60‑1 (MQ=255) gTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtct > 1:715040/1‑71 (MQ=255) tAAAAACAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAtc < 1:551551/69‑1 (MQ=255) tAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCtt < 1:1775155/47‑1 (MQ=255) aaaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATc < 1:2099121/62‑1 (MQ=255) aaaaCAGTAATCACATTTGAGATTACATTGAAAACTGGa > 1:28418/1‑39 (MQ=255) aaaaCAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGc > 1:995289/1‑71 (MQ=255) aaCAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCtt > 1:1477166/1‑43 (MQ=255) aaCAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCt > 1:2626487/1‑70 (MQ=255) aaCAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCt > 1:2115602/1‑70 (MQ=255) cAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCaa > 1:4014143/1‑71 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCtt < 1:3443342/38‑1 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCa > 1:2686511/1‑52 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAt > 1:1825941/1‑59 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAt > 1:1188108/1‑59 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt > 1:695369/1‑69 (MQ=255) tAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATaa < 1:511632/71‑1 (MQ=255) aaCACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAgg > 1:908820/1‑71 (MQ=255) acacATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTAt > 1:3874997/1‑56 (MQ=255) acacATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt < 1:142486/66‑1 (MQ=255) acacATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAAt < 1:1296279/66‑1 (MQ=255) | TCAGAGTGCTGTCATCCATCCATTTTTCATGAGTAAACTGCGACTCTTTTTCAAGGTAAAAACAGTAAACACATTTGAGATTACATTGAAAACTGGAGGGCTTGGCTGTAACGTGCATCGCTATCTCGCTCAATAAGG > minE/965776‑965913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |